Dengue type 4 in Rio de Janeiro, Brazil: case characterization following its introduction in an endemic region
Abstract Background Due to the populations’ susceptibility, DENV-4 introduction in 2010 led to the occurrence of explosive epidemics in the following years in Brazil. In 2011, DENV-4 was identified in Rio de Janeiro (RJ) and it was prevalent in 2012 and 2013. Here, we aimed to characterize clinical,...
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| Format: | Article |
| Language: | English |
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BMC
2017-06-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | http://link.springer.com/article/10.1186/s12879-017-2488-4 |
| _version_ | 1818600405179826176 |
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| author | Manoela Heringer Thiara Manuele A Souza Monique da Rocha Q Lima Priscila Conrado G Nunes Nieli Rodrigues da C Faria Fernanda de Bruycker-Nogueira Thaís Chouin-Carneiro Rita Maria R Nogueira Flavia Barreto dos Santos |
| author_facet | Manoela Heringer Thiara Manuele A Souza Monique da Rocha Q Lima Priscila Conrado G Nunes Nieli Rodrigues da C Faria Fernanda de Bruycker-Nogueira Thaís Chouin-Carneiro Rita Maria R Nogueira Flavia Barreto dos Santos |
| author_sort | Manoela Heringer |
| collection | DOAJ |
| description | Abstract Background Due to the populations’ susceptibility, DENV-4 introduction in 2010 led to the occurrence of explosive epidemics in the following years in Brazil. In 2011, DENV-4 was identified in Rio de Janeiro (RJ) and it was prevalent in 2012 and 2013. Here, we aimed to characterize clinical, epidemiological and laboratorial aspects of DENV-4 cases after this serotype introduction in an endemic scenario. Methods Dengue suspected cases (n = 3727) were received and analyzed from January 2011 to December 2013, during outbreaks occurred in RJ, Brazil. Samples were submitted to virological, serological and molecular methods for case confirmation. DENV-4 cases (n = 705) were characterized according to the type of infection, disease severity and, viremia levels and NS1 antigenemia were accessed. Representative strains were partial sequenced for genotyping. Results DENV-4 was identified in 44.2% (705/1593) of dengue positive cases, virus isolated in 48.7% of the cases. Anti-DENV IgM was detected in 39.4% of the cases, however an increased detection was observed in cases with ≥4 days of symptoms (57.0%). NS1 antigen was identified in 41.5% of DENV-4 cases however, after immune complexes dissociation, the detection significantly increased (87.6%). Females were more affected than males, so did children aged 11–15 years old. Primary cases were more frequently observed than secondary ones and most of them were classified as dengue. No differences on NS1 antigenemia and viraemia within the groups were observed. Despite the higher frequency of severe disease on individuals >65 years old, no differences were observed among the groups and type of infection. However, DENV-4 fatal cases were more frequent on secondary infections (57.1%). DENV-4 Genotype II was identified with a probable origin from Venezuela and Colombia. Conclusions It has been shown that laboratorial diagnosis is still a reliable tool for the disease surveillance, detecting and confirming emerging epidemics. Despite the occurrence of secondary infections, most DENV-4 cases presented a mild disease. As RJ is endemic for dengue, high rates of secondary infections would be expected. Despite the existence of two genotypes, only Genotype II was identified in our study. |
| first_indexed | 2024-12-16T12:34:58Z |
| format | Article |
| id | doaj.art-ea401401c081472baa7570e628cfea52 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-16T12:34:58Z |
| publishDate | 2017-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-ea401401c081472baa7570e628cfea522022-12-21T22:31:36ZengBMCBMC Infectious Diseases1471-23342017-06-011711910.1186/s12879-017-2488-4Dengue type 4 in Rio de Janeiro, Brazil: case characterization following its introduction in an endemic regionManoela Heringer0Thiara Manuele A Souza1Monique da Rocha Q Lima2Priscila Conrado G Nunes3Nieli Rodrigues da C Faria4Fernanda de Bruycker-Nogueira5Thaís Chouin-Carneiro6Rita Maria R Nogueira7Flavia Barreto dos Santos8Viral Immunology Laboratory, Oswaldo Cruz Institute/FIOCRUZViral Immunology Laboratory, Oswaldo Cruz Institute/FIOCRUZViral Immunology Laboratory, Oswaldo Cruz Institute/FIOCRUZViral Immunology Laboratory, Oswaldo Cruz Institute/FIOCRUZViral Immunology Laboratory, Oswaldo Cruz Institute/FIOCRUZViral Immunology Laboratory, Oswaldo Cruz Institute/FIOCRUZViral Immunology Laboratory, Oswaldo Cruz Institute/FIOCRUZFlavivirus Laboratory, Oswaldo Cruz InstituteViral Immunology Laboratory, Oswaldo Cruz Institute/FIOCRUZAbstract Background Due to the populations’ susceptibility, DENV-4 introduction in 2010 led to the occurrence of explosive epidemics in the following years in Brazil. In 2011, DENV-4 was identified in Rio de Janeiro (RJ) and it was prevalent in 2012 and 2013. Here, we aimed to characterize clinical, epidemiological and laboratorial aspects of DENV-4 cases after this serotype introduction in an endemic scenario. Methods Dengue suspected cases (n = 3727) were received and analyzed from January 2011 to December 2013, during outbreaks occurred in RJ, Brazil. Samples were submitted to virological, serological and molecular methods for case confirmation. DENV-4 cases (n = 705) were characterized according to the type of infection, disease severity and, viremia levels and NS1 antigenemia were accessed. Representative strains were partial sequenced for genotyping. Results DENV-4 was identified in 44.2% (705/1593) of dengue positive cases, virus isolated in 48.7% of the cases. Anti-DENV IgM was detected in 39.4% of the cases, however an increased detection was observed in cases with ≥4 days of symptoms (57.0%). NS1 antigen was identified in 41.5% of DENV-4 cases however, after immune complexes dissociation, the detection significantly increased (87.6%). Females were more affected than males, so did children aged 11–15 years old. Primary cases were more frequently observed than secondary ones and most of them were classified as dengue. No differences on NS1 antigenemia and viraemia within the groups were observed. Despite the higher frequency of severe disease on individuals >65 years old, no differences were observed among the groups and type of infection. However, DENV-4 fatal cases were more frequent on secondary infections (57.1%). DENV-4 Genotype II was identified with a probable origin from Venezuela and Colombia. Conclusions It has been shown that laboratorial diagnosis is still a reliable tool for the disease surveillance, detecting and confirming emerging epidemics. Despite the occurrence of secondary infections, most DENV-4 cases presented a mild disease. As RJ is endemic for dengue, high rates of secondary infections would be expected. Despite the existence of two genotypes, only Genotype II was identified in our study.http://link.springer.com/article/10.1186/s12879-017-2488-4Dengue virus type 4Laboratorial diagnosisPhylogenyEndemicRio de JaneiroBrazil |
| spellingShingle | Manoela Heringer Thiara Manuele A Souza Monique da Rocha Q Lima Priscila Conrado G Nunes Nieli Rodrigues da C Faria Fernanda de Bruycker-Nogueira Thaís Chouin-Carneiro Rita Maria R Nogueira Flavia Barreto dos Santos Dengue type 4 in Rio de Janeiro, Brazil: case characterization following its introduction in an endemic region BMC Infectious Diseases Dengue virus type 4 Laboratorial diagnosis Phylogeny Endemic Rio de Janeiro Brazil |
| title | Dengue type 4 in Rio de Janeiro, Brazil: case characterization following its introduction in an endemic region |
| title_full | Dengue type 4 in Rio de Janeiro, Brazil: case characterization following its introduction in an endemic region |
| title_fullStr | Dengue type 4 in Rio de Janeiro, Brazil: case characterization following its introduction in an endemic region |
| title_full_unstemmed | Dengue type 4 in Rio de Janeiro, Brazil: case characterization following its introduction in an endemic region |
| title_short | Dengue type 4 in Rio de Janeiro, Brazil: case characterization following its introduction in an endemic region |
| title_sort | dengue type 4 in rio de janeiro brazil case characterization following its introduction in an endemic region |
| topic | Dengue virus type 4 Laboratorial diagnosis Phylogeny Endemic Rio de Janeiro Brazil |
| url | http://link.springer.com/article/10.1186/s12879-017-2488-4 |
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