Facile preparation of silver based radiosensitizers via biomineralization method for enhanced in vivo breast cancer radiotherapy
Abstract To solve the traditional radiotherapy obstacles, and also to enhance the radiation therapy efficacy various radiosensitizers have been developed. Radiosensitizers are promising agents that under X-ray irradiation enhance injury to tumor tissue by accelerating DNA damage. In this report, sil...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-40763-9 |
| _version_ | 1797452820853555200 |
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| author | Mohammadreza Ghaffarlou Ali Mohammadi Navid Mousazadeh Marziyeh Salehiabar Yahya Kalantari Jalil Charmi Murat Barsbay Yavuz Nuri Ertas Hossein Danafar Hamed Rezaeejam Hamed Nosrati Siamak Javani |
| author_facet | Mohammadreza Ghaffarlou Ali Mohammadi Navid Mousazadeh Marziyeh Salehiabar Yahya Kalantari Jalil Charmi Murat Barsbay Yavuz Nuri Ertas Hossein Danafar Hamed Rezaeejam Hamed Nosrati Siamak Javani |
| author_sort | Mohammadreza Ghaffarlou |
| collection | DOAJ |
| description | Abstract To solve the traditional radiotherapy obstacles, and also to enhance the radiation therapy efficacy various radiosensitizers have been developed. Radiosensitizers are promising agents that under X-ray irradiation enhance injury to tumor tissue by accelerating DNA damage. In this report, silver-silver sulfide nanoparticles (Ag-Ag2S NPs) were synthesized via a facile, one-pot and environmentally friendly biomineralization method. Ag-Ag2S was coated with bovine serum albumin (BSA) in situ and applied as an X-ray sensitizer to enhance the efficiency of radiotherapy. Also, folic acid (FA) was conjugated to Ag-Ag2S@BSA to impart active targeting capability to the final formulation (Ag-Ag2S@BSA-FA). Prepared NPs were characterized by transmission electron microscopes (TEM), scanning electron microscope (SEM), dynamic light scattering (DLS), ultraviolet–visible spectroscopy (UV–Vis), X-ray diffraction analysis (XRD), and X-ray photoelectron spectroscopy (XPS) techniques. Results show that most of the NPs have well-defined uniform Janus structures. The biocompatibility of the NPs was then evaluated both in vitro and in vivo. A series of in vitro assays were performed on 4T1 cancer cells to evaluate the therapeutic efficacy of the designed NPs. In addition, the radio-enhancing ability of the NPs was tested on the 4T1 breast cancer murine model. MTT, live and dead cell staining, apoptosis, ROS generation, and clonogenic in vitro assays demonstrated the efficacy of NPs as radiosensitizers in radiotherapy. In vivo results as well as H&E staining tumor tissues confirmed tumor destruction in the group that received Ag-Ag2S@BSA-FA NPs and exposed to X-ray. The results showed that prepared tumor-targeted Ag-Ag2S@BSA-FA NPs could be potential candidates as radiosensitizers for enhanced radiotherapy. |
| first_indexed | 2024-03-09T15:14:12Z |
| format | Article |
| id | doaj.art-ea45d98782904f9da4e8f5b2d8294fbe |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:14:12Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ea45d98782904f9da4e8f5b2d8294fbe2023-11-26T13:12:00ZengNature PortfolioScientific Reports2045-23222023-09-0113111210.1038/s41598-023-40763-9Facile preparation of silver based radiosensitizers via biomineralization method for enhanced in vivo breast cancer radiotherapyMohammadreza Ghaffarlou0Ali Mohammadi1Navid Mousazadeh2Marziyeh Salehiabar3Yahya Kalantari4Jalil Charmi5Murat Barsbay6Yavuz Nuri Ertas7Hossein Danafar8Hamed Rezaeejam9Hamed Nosrati10Siamak Javani11Department of Chemistry, Hacettepe UniversityZanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical SciencesZanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical SciencesZanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical SciencesZanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical SciencesZanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical SciencesDepartment of Chemistry, Hacettepe UniversityERNAM-Nanotechnology Research and Application Center, Erciyes UniversityZanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical SciencesDepartment of Radiology Technology, School of Allied Medical Sciences, Zanjan University of Medical SciencesZanjan Pharmaceutical Biotechnology Research Center, Zanjan University of Medical SciencesMedical Cellular and Molecular Research Center, Golestan University of Medical SciencesAbstract To solve the traditional radiotherapy obstacles, and also to enhance the radiation therapy efficacy various radiosensitizers have been developed. Radiosensitizers are promising agents that under X-ray irradiation enhance injury to tumor tissue by accelerating DNA damage. In this report, silver-silver sulfide nanoparticles (Ag-Ag2S NPs) were synthesized via a facile, one-pot and environmentally friendly biomineralization method. Ag-Ag2S was coated with bovine serum albumin (BSA) in situ and applied as an X-ray sensitizer to enhance the efficiency of radiotherapy. Also, folic acid (FA) was conjugated to Ag-Ag2S@BSA to impart active targeting capability to the final formulation (Ag-Ag2S@BSA-FA). Prepared NPs were characterized by transmission electron microscopes (TEM), scanning electron microscope (SEM), dynamic light scattering (DLS), ultraviolet–visible spectroscopy (UV–Vis), X-ray diffraction analysis (XRD), and X-ray photoelectron spectroscopy (XPS) techniques. Results show that most of the NPs have well-defined uniform Janus structures. The biocompatibility of the NPs was then evaluated both in vitro and in vivo. A series of in vitro assays were performed on 4T1 cancer cells to evaluate the therapeutic efficacy of the designed NPs. In addition, the radio-enhancing ability of the NPs was tested on the 4T1 breast cancer murine model. MTT, live and dead cell staining, apoptosis, ROS generation, and clonogenic in vitro assays demonstrated the efficacy of NPs as radiosensitizers in radiotherapy. In vivo results as well as H&E staining tumor tissues confirmed tumor destruction in the group that received Ag-Ag2S@BSA-FA NPs and exposed to X-ray. The results showed that prepared tumor-targeted Ag-Ag2S@BSA-FA NPs could be potential candidates as radiosensitizers for enhanced radiotherapy.https://doi.org/10.1038/s41598-023-40763-9 |
| spellingShingle | Mohammadreza Ghaffarlou Ali Mohammadi Navid Mousazadeh Marziyeh Salehiabar Yahya Kalantari Jalil Charmi Murat Barsbay Yavuz Nuri Ertas Hossein Danafar Hamed Rezaeejam Hamed Nosrati Siamak Javani Facile preparation of silver based radiosensitizers via biomineralization method for enhanced in vivo breast cancer radiotherapy Scientific Reports |
| title | Facile preparation of silver based radiosensitizers via biomineralization method for enhanced in vivo breast cancer radiotherapy |
| title_full | Facile preparation of silver based radiosensitizers via biomineralization method for enhanced in vivo breast cancer radiotherapy |
| title_fullStr | Facile preparation of silver based radiosensitizers via biomineralization method for enhanced in vivo breast cancer radiotherapy |
| title_full_unstemmed | Facile preparation of silver based radiosensitizers via biomineralization method for enhanced in vivo breast cancer radiotherapy |
| title_short | Facile preparation of silver based radiosensitizers via biomineralization method for enhanced in vivo breast cancer radiotherapy |
| title_sort | facile preparation of silver based radiosensitizers via biomineralization method for enhanced in vivo breast cancer radiotherapy |
| url | https://doi.org/10.1038/s41598-023-40763-9 |
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