The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats
The anti-inflammatory and cholesterol lowering capabilities of probiotic bacteria highlight them as potential prophylactics against chronic inflammatory diseases, particularly cardiovascular disease. Previous studies in silico, in vitro, and in vivo suggest that the Lab4 probiotic consortium may har...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-11-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2021.778289/full |
| _version_ | 1819038972752429056 |
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| author | Thomas S. Webberley Giulia Masetti Laura M. Baker Jordanna Dally Timothy R. Hughes Julian R. Marchesi Alison A. Jack Sue F. Plummer Guru Ramanathan Paul D. Facey Daryn R. Michael |
| author_facet | Thomas S. Webberley Giulia Masetti Laura M. Baker Jordanna Dally Timothy R. Hughes Julian R. Marchesi Alison A. Jack Sue F. Plummer Guru Ramanathan Paul D. Facey Daryn R. Michael |
| author_sort | Thomas S. Webberley |
| collection | DOAJ |
| description | The anti-inflammatory and cholesterol lowering capabilities of probiotic bacteria highlight them as potential prophylactics against chronic inflammatory diseases, particularly cardiovascular disease. Previous studies in silico, in vitro, and in vivo suggest that the Lab4 probiotic consortium may harbour such capabilities and in the current study, we assessed plasma levels of cytokines/chemokines, short chain fatty acids and lipids and faecal levels of bile acids in a subpopulation of healthy Wistar rats included in 90-day repeat dose oral toxicity study. In the rats receiving Lab4, circulating levels of pro-inflammatory interleukin-6, tumour necrosis factor-α and keratinocyte chemoattractant/growth regulated oncogene were significantly lower compared to the control group demonstrating a systemic anti-inflammatory effect. These changes occurred alongside significant reductions in plasma low density lipoprotein cholesterol and increases in faecal bile acid excretion implying the ability to lower circulating cholesterol via the deconjugation of intestinal bile acids. Correlative analysis identified significant associations between plasma tumour necrosis factor-α and the plasma total cholesterol:high density lipoprotein cholesterol ratio and faecal levels of bifidobacteria in the Lab4 rats. Together, these data highlight Lab4 supplementation as a holistic approach to CVD prevention and encourages further studies in humans. |
| first_indexed | 2024-12-21T08:45:48Z |
| format | Article |
| id | doaj.art-ea4801e0729649bd94e425beaf15c7f5 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-12-21T08:45:48Z |
| publishDate | 2021-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-ea4801e0729649bd94e425beaf15c7f52022-12-21T19:09:51ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2021-11-01810.3389/fnut.2021.778289778289The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar RatsThomas S. Webberley0Giulia Masetti1Laura M. Baker2Jordanna Dally3Timothy R. Hughes4Julian R. Marchesi5Alison A. Jack6Sue F. Plummer7Guru Ramanathan8Paul D. Facey9Daryn R. Michael10Cultech Limited, Port Talbot, United KingdomCultech Limited, Port Talbot, United KingdomSwansea University Medical School, Swansea University, Swansea, United KingdomCultech Limited, Port Talbot, United KingdomDivision of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, United KingdomDivision of Digestive Diseases, Department of Metabolism, Digestion and Reproduction, Faculty of Medicine, Imperial College London, London, United KingdomCultech Limited, Port Talbot, United KingdomCultech Limited, Port Talbot, United KingdomPharmacology based Clinical Trials, Pennington Biomedical Research Centre, Baton Rouge, LA, United StatesSwansea University Medical School, Swansea University, Swansea, United KingdomCultech Limited, Port Talbot, United KingdomThe anti-inflammatory and cholesterol lowering capabilities of probiotic bacteria highlight them as potential prophylactics against chronic inflammatory diseases, particularly cardiovascular disease. Previous studies in silico, in vitro, and in vivo suggest that the Lab4 probiotic consortium may harbour such capabilities and in the current study, we assessed plasma levels of cytokines/chemokines, short chain fatty acids and lipids and faecal levels of bile acids in a subpopulation of healthy Wistar rats included in 90-day repeat dose oral toxicity study. In the rats receiving Lab4, circulating levels of pro-inflammatory interleukin-6, tumour necrosis factor-α and keratinocyte chemoattractant/growth regulated oncogene were significantly lower compared to the control group demonstrating a systemic anti-inflammatory effect. These changes occurred alongside significant reductions in plasma low density lipoprotein cholesterol and increases in faecal bile acid excretion implying the ability to lower circulating cholesterol via the deconjugation of intestinal bile acids. Correlative analysis identified significant associations between plasma tumour necrosis factor-α and the plasma total cholesterol:high density lipoprotein cholesterol ratio and faecal levels of bifidobacteria in the Lab4 rats. Together, these data highlight Lab4 supplementation as a holistic approach to CVD prevention and encourages further studies in humans.https://www.frontiersin.org/articles/10.3389/fnut.2021.778289/fullprobioticinflammationSCFAcholesterolbilecardiovascular |
| spellingShingle | Thomas S. Webberley Giulia Masetti Laura M. Baker Jordanna Dally Timothy R. Hughes Julian R. Marchesi Alison A. Jack Sue F. Plummer Guru Ramanathan Paul D. Facey Daryn R. Michael The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats Frontiers in Nutrition probiotic inflammation SCFA cholesterol bile cardiovascular |
| title | The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats |
| title_full | The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats |
| title_fullStr | The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats |
| title_full_unstemmed | The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats |
| title_short | The Impact of Lab4 Probiotic Supplementation in a 90-Day Study in Wistar Rats |
| title_sort | impact of lab4 probiotic supplementation in a 90 day study in wistar rats |
| topic | probiotic inflammation SCFA cholesterol bile cardiovascular |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2021.778289/full |
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