Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial Cystitis
Purpose: The aim of this study was to evaluate changes in expressions of neuregulin (NRG)1 and erbB2 tyrosine kinase (ErbB2) in bladders of rats with cyclophosphamide (CYP)-induced interstitial cystitis (IC). Methods: Twenty-four Sprague-Dawley rats were divided into the IC group (n=16) and the cont...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Korean Continence Society
2015-09-01
|
| Series: | International Neurourology Journal |
| Subjects: | |
| Online Access: | http://www.einj.org/upload/pdf/inj-19-3-158.pdf |
| _version_ | 1818231703174381568 |
|---|---|
| author | Ki Hak Song Chang Shik Youn Chung Lyul Lee Seung Woo Yang Young Seop Chang Seoung Woo Jeong Chong Koo Sul |
| author_facet | Ki Hak Song Chang Shik Youn Chung Lyul Lee Seung Woo Yang Young Seop Chang Seoung Woo Jeong Chong Koo Sul |
| author_sort | Ki Hak Song |
| collection | DOAJ |
| description | Purpose: The aim of this study was to evaluate changes in expressions of neuregulin (NRG)1 and erbB2 tyrosine kinase (ErbB2) in bladders of rats with cyclophosphamide (CYP)-induced interstitial cystitis (IC). Methods: Twenty-four Sprague-Dawley rats were divided into the IC group (n=16) and the control group (n=8). After inducing IC with intraperitoneal CYP injection, expressions of NRG1 and ErbB2 were analyzed using western blotting and reverse transcriptase-polymerase chain reaction. Results: In Western blotting, relative intensities and distributions of both NRG1 and ErbB2 were approximately 1.5- and 3.2-fold higher, respectively, in the IC group than in the control group (mean±standard deviation: 1.42±0.09 vs. 0.93±0.15 and 0.93±0.16 vs. 0.29±0.08, P<0.05). In the rat bladder samples, mRNA expression levels of NRG1 and ErbB2 were higher in the IC group than in the control group (P<0.05). Conclusions: Our study has demonstrated significant changes in mRNA expression and immunoreactivity of NRG1 and ErbB2 receptors in the urinary bladder after CYP-induced IC. These results suggest that the up-regulated NRG1 may play a role in inducing an overactive bladder and promoting regeneration in the inflammatory bladder with CYP-induced IC. |
| first_indexed | 2024-12-12T10:54:36Z |
| format | Article |
| id | doaj.art-ea49eaea4f704724907dbacb16874378 |
| institution | Directory Open Access Journal |
| issn | 2093-4777 2093-6931 |
| language | English |
| last_indexed | 2024-12-12T10:54:36Z |
| publishDate | 2015-09-01 |
| publisher | Korean Continence Society |
| record_format | Article |
| series | International Neurourology Journal |
| spelling | doaj.art-ea49eaea4f704724907dbacb168743782022-12-22T00:26:41ZengKorean Continence SocietyInternational Neurourology Journal2093-47772093-69312015-09-0119315816310.5213/inj.2015.19.3.158537Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial CystitisKi Hak Song0Chang Shik Youn1Chung Lyul Lee2Seung Woo Yang3Young Seop Chang4Seoung Woo Jeong5Chong Koo Sul6 Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea Department of Urology, The Catholic University of Korea, Daejeon St. Mary’s Hospital, Daejeon, Korea Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea Department of Urology, Chungnam National University School of Medicine, Daejeon, Korea Department of Urology, Konyang University College of Medicine, Daejeon, Korea Department of Physiology, Yonsei University Wonju College of Medicine, Wonju, Korea Department of Urology, Chungnam National University School of Medicine, Daejeon, KoreaPurpose: The aim of this study was to evaluate changes in expressions of neuregulin (NRG)1 and erbB2 tyrosine kinase (ErbB2) in bladders of rats with cyclophosphamide (CYP)-induced interstitial cystitis (IC). Methods: Twenty-four Sprague-Dawley rats were divided into the IC group (n=16) and the control group (n=8). After inducing IC with intraperitoneal CYP injection, expressions of NRG1 and ErbB2 were analyzed using western blotting and reverse transcriptase-polymerase chain reaction. Results: In Western blotting, relative intensities and distributions of both NRG1 and ErbB2 were approximately 1.5- and 3.2-fold higher, respectively, in the IC group than in the control group (mean±standard deviation: 1.42±0.09 vs. 0.93±0.15 and 0.93±0.16 vs. 0.29±0.08, P<0.05). In the rat bladder samples, mRNA expression levels of NRG1 and ErbB2 were higher in the IC group than in the control group (P<0.05). Conclusions: Our study has demonstrated significant changes in mRNA expression and immunoreactivity of NRG1 and ErbB2 receptors in the urinary bladder after CYP-induced IC. These results suggest that the up-regulated NRG1 may play a role in inducing an overactive bladder and promoting regeneration in the inflammatory bladder with CYP-induced IC.http://www.einj.org/upload/pdf/inj-19-3-158.pdfNeuregulin-1Receptor, ErbB-2Cystitis, InterstitialRats |
| spellingShingle | Ki Hak Song Chang Shik Youn Chung Lyul Lee Seung Woo Yang Young Seop Chang Seoung Woo Jeong Chong Koo Sul Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial Cystitis International Neurourology Journal Neuregulin-1 Receptor, ErbB-2 Cystitis, Interstitial Rats |
| title | Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial Cystitis |
| title_full | Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial Cystitis |
| title_fullStr | Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial Cystitis |
| title_full_unstemmed | Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial Cystitis |
| title_short | Increased Expression of Neuregulin 1 and erbB2 Tyrosine Kinase in the Bladder of Rats With Cyclophosphamide-Induced Interstitial Cystitis |
| title_sort | increased expression of neuregulin 1 and erbb2 tyrosine kinase in the bladder of rats with cyclophosphamide induced interstitial cystitis |
| topic | Neuregulin-1 Receptor, ErbB-2 Cystitis, Interstitial Rats |
| url | http://www.einj.org/upload/pdf/inj-19-3-158.pdf |
| work_keys_str_mv | AT kihaksong increasedexpressionofneuregulin1anderbb2tyrosinekinaseinthebladderofratswithcyclophosphamideinducedinterstitialcystitis AT changshikyoun increasedexpressionofneuregulin1anderbb2tyrosinekinaseinthebladderofratswithcyclophosphamideinducedinterstitialcystitis AT chunglyullee increasedexpressionofneuregulin1anderbb2tyrosinekinaseinthebladderofratswithcyclophosphamideinducedinterstitialcystitis AT seungwooyang increasedexpressionofneuregulin1anderbb2tyrosinekinaseinthebladderofratswithcyclophosphamideinducedinterstitialcystitis AT youngseopchang increasedexpressionofneuregulin1anderbb2tyrosinekinaseinthebladderofratswithcyclophosphamideinducedinterstitialcystitis AT seoungwoojeong increasedexpressionofneuregulin1anderbb2tyrosinekinaseinthebladderofratswithcyclophosphamideinducedinterstitialcystitis AT chongkoosul increasedexpressionofneuregulin1anderbb2tyrosinekinaseinthebladderofratswithcyclophosphamideinducedinterstitialcystitis |