Genetic and phylogenetic relationship of an American caprine rotavirus B strain with equine rotavirus B
Rotaviruses (RVs) are significant enteric pathogens of humans and animals. In March 2021, the University of Kentucky Veterinary Diagnostic Laboratory (UKVDL) received a fecal sample from a 1-week-old goat kid with diarrhea from a farm with 5 additional diarrheic kids. The fecal sample was tested neg...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Virology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fviro.2023.1181017/full |
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| author | Litty Paul Jocelynn Morgan Gloria Pulley Tirth Uprety Ben M. Hause Emma Adam Feng Li Craig N. Carter Douglas Marthaler Erdal Erol |
| author_facet | Litty Paul Jocelynn Morgan Gloria Pulley Tirth Uprety Ben M. Hause Emma Adam Feng Li Craig N. Carter Douglas Marthaler Erdal Erol |
| author_sort | Litty Paul |
| collection | DOAJ |
| description | Rotaviruses (RVs) are significant enteric pathogens of humans and animals. In March 2021, the University of Kentucky Veterinary Diagnostic Laboratory (UKVDL) received a fecal sample from a 1-week-old goat kid with diarrhea from a farm with 5 additional diarrheic kids. The fecal sample was tested negative for Bovine coronavirus, Bovine rotavirus Group A, E. coli K99+, Cryptosporidium parvum and Salmonella spp by multiplex real-time PCR assays. Interestingly, a novel Equine Rotavirus B (ERVB) in Kentucky was identified from neonatal foals also with watery diarrhea in the Spring of 2021. Once the ERVB-specific real-time PCR assay became available, the fecal sample from the goat kid was tested and found positive for RVB. Genome sequence of the caprine RVB from fecal sample was obtained using shotgun metagenomic sequencing by Illumina MiSeq. All of the eleven viral segments of caprine RVB were sequenced either completely or partially. Genetic and phylogenetic analysis of VP7, VP4, VP6, VP1–VP3, and NSP1- NSP5 genes indicated the caprine RVB strain had the genotype constellation of G3-P[3]-I3- R3-C3-M3-A4-N3-T3-E3-H3. In addition, the caprine RVB sequence showed the highest nucleotide identity and evolutionary relationship to the ERVB and previously sequenced caprine RVB strains. Given the similar geographical location of the equine and caprine strains from our study, these findings suggest a possible common source of infection. |
| first_indexed | 2024-03-13T09:38:54Z |
| format | Article |
| id | doaj.art-ea4bbb08243e4195b7f1c8e162b0ce3e |
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| issn | 2673-818X |
| language | English |
| last_indexed | 2024-03-13T09:38:54Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Virology |
| spelling | doaj.art-ea4bbb08243e4195b7f1c8e162b0ce3e2023-05-25T04:59:13ZengFrontiers Media S.A.Frontiers in Virology2673-818X2023-05-01310.3389/fviro.2023.11810171181017Genetic and phylogenetic relationship of an American caprine rotavirus B strain with equine rotavirus BLitty Paul0Jocelynn Morgan1Gloria Pulley2Tirth Uprety3Ben M. Hause4Emma Adam5Feng Li6Craig N. Carter7Douglas Marthaler8Erdal Erol9Veterinary Diagnostic Laboratory, University of Kentucky, Lexington, KY, United StatesVeterinary Diagnostic Laboratory, University of Kentucky, Lexington, KY, United StatesVeterinary Diagnostic Laboratory, University of Kentucky, Lexington, KY, United StatesMaxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, KY, United StatesDepartment of Veterinary and Biomedical Sciences, South Dakota State University, Brookings, SD, United StatesMaxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, KY, United StatesMaxwell H. Gluck Equine Research Center, University of Kentucky, Lexington, KY, United StatesVeterinary Diagnostic Laboratory, University of Kentucky, Lexington, KY, United StatesScience and Technology, Indical Inc., Orlando, FL, United StatesVeterinary Diagnostic Laboratory, University of Kentucky, Lexington, KY, United StatesRotaviruses (RVs) are significant enteric pathogens of humans and animals. In March 2021, the University of Kentucky Veterinary Diagnostic Laboratory (UKVDL) received a fecal sample from a 1-week-old goat kid with diarrhea from a farm with 5 additional diarrheic kids. The fecal sample was tested negative for Bovine coronavirus, Bovine rotavirus Group A, E. coli K99+, Cryptosporidium parvum and Salmonella spp by multiplex real-time PCR assays. Interestingly, a novel Equine Rotavirus B (ERVB) in Kentucky was identified from neonatal foals also with watery diarrhea in the Spring of 2021. Once the ERVB-specific real-time PCR assay became available, the fecal sample from the goat kid was tested and found positive for RVB. Genome sequence of the caprine RVB from fecal sample was obtained using shotgun metagenomic sequencing by Illumina MiSeq. All of the eleven viral segments of caprine RVB were sequenced either completely or partially. Genetic and phylogenetic analysis of VP7, VP4, VP6, VP1–VP3, and NSP1- NSP5 genes indicated the caprine RVB strain had the genotype constellation of G3-P[3]-I3- R3-C3-M3-A4-N3-T3-E3-H3. In addition, the caprine RVB sequence showed the highest nucleotide identity and evolutionary relationship to the ERVB and previously sequenced caprine RVB strains. Given the similar geographical location of the equine and caprine strains from our study, these findings suggest a possible common source of infection.https://www.frontiersin.org/articles/10.3389/fviro.2023.1181017/fullfoalgoat kidphylogeneticrotavirus Bsequencing |
| spellingShingle | Litty Paul Jocelynn Morgan Gloria Pulley Tirth Uprety Ben M. Hause Emma Adam Feng Li Craig N. Carter Douglas Marthaler Erdal Erol Genetic and phylogenetic relationship of an American caprine rotavirus B strain with equine rotavirus B Frontiers in Virology foal goat kid phylogenetic rotavirus B sequencing |
| title | Genetic and phylogenetic relationship of an American caprine rotavirus B strain with equine rotavirus B |
| title_full | Genetic and phylogenetic relationship of an American caprine rotavirus B strain with equine rotavirus B |
| title_fullStr | Genetic and phylogenetic relationship of an American caprine rotavirus B strain with equine rotavirus B |
| title_full_unstemmed | Genetic and phylogenetic relationship of an American caprine rotavirus B strain with equine rotavirus B |
| title_short | Genetic and phylogenetic relationship of an American caprine rotavirus B strain with equine rotavirus B |
| title_sort | genetic and phylogenetic relationship of an american caprine rotavirus b strain with equine rotavirus b |
| topic | foal goat kid phylogenetic rotavirus B sequencing |
| url | https://www.frontiersin.org/articles/10.3389/fviro.2023.1181017/full |
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