Aptamer Validation by Western Blot–an overview
Western blot is the main and basic technique in cellular and molecular biology. The principle of the western blot is the isolation and detection of the target molecule usually from a cellular extract. The whole process of western blot consists of three stages and can be described briefly as separat...
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| Format: | Article |
| Language: | English |
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College of Pharmacy / Mustansiriyah University
2022-04-01
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| Series: | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
| Online Access: | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/782 |
| _version_ | 1797223964281405440 |
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| author | Lamees Jamal Talib Basma Talib Al-Sudani Mustafa Ghazi Al-Abbassi |
| author_facet | Lamees Jamal Talib Basma Talib Al-Sudani Mustafa Ghazi Al-Abbassi |
| author_sort | Lamees Jamal Talib |
| collection | DOAJ |
| description |
Western blot is the main and basic technique in cellular and molecular biology. The principle of the western blot is the isolation and detection of the target molecule usually from a cellular extract. The whole process of western blot consists of three stages and can be described briefly as separation of
protein. followed by transportation to a solid membrane and finally detection of the target by an antibody. Western blot technique is usually used for the detection of proteins but also can be used to detect other molecules such as aptamers. Aptamers can be defined as a short-stranded DNA or RNA that bind with the target with high specificity and affinity. Aptamers highly resemble antibodies with many advantages. In this review, there is a focus on the aptamers that had validated by western blot technique other than other methods. This method has the advantage of less time required, no antibodies needed, and introducing the possibility of multiplexing detection.
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| first_indexed | 2024-04-24T13:45:35Z |
| format | Article |
| id | doaj.art-ea4bc0edf13e46f598ce8605361c0d00 |
| institution | Directory Open Access Journal |
| issn | 1815-0993 2959-183X |
| language | English |
| last_indexed | 2024-04-24T13:45:35Z |
| publishDate | 2022-04-01 |
| publisher | College of Pharmacy / Mustansiriyah University |
| record_format | Article |
| series | Al-Mustansiriyah Journal of Pharmaceutical Sciences |
| spelling | doaj.art-ea4bc0edf13e46f598ce8605361c0d002024-04-04T06:57:40ZengCollege of Pharmacy / Mustansiriyah UniversityAl-Mustansiriyah Journal of Pharmaceutical Sciences1815-09932959-183X2022-04-0120410.32947/ajps.v20i4.782Aptamer Validation by Western Blot–an overviewLamees Jamal Talib 0Basma Talib Al-Sudani 1Mustafa Ghazi Al-Abbassi2Central Teaching Hospital of Pediatric, Baghdad, IraqDepartment of Clinical Laboratory Sciences, College of Pharmacy, Mustansiriyah University, Baghdad, IraqCollege of Pharmacy, Alkafeel University, Iraq Western blot is the main and basic technique in cellular and molecular biology. The principle of the western blot is the isolation and detection of the target molecule usually from a cellular extract. The whole process of western blot consists of three stages and can be described briefly as separation of protein. followed by transportation to a solid membrane and finally detection of the target by an antibody. Western blot technique is usually used for the detection of proteins but also can be used to detect other molecules such as aptamers. Aptamers can be defined as a short-stranded DNA or RNA that bind with the target with high specificity and affinity. Aptamers highly resemble antibodies with many advantages. In this review, there is a focus on the aptamers that had validated by western blot technique other than other methods. This method has the advantage of less time required, no antibodies needed, and introducing the possibility of multiplexing detection. https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/782 |
| spellingShingle | Lamees Jamal Talib Basma Talib Al-Sudani Mustafa Ghazi Al-Abbassi Aptamer Validation by Western Blot–an overview Al-Mustansiriyah Journal of Pharmaceutical Sciences |
| title | Aptamer Validation by Western Blot–an overview |
| title_full | Aptamer Validation by Western Blot–an overview |
| title_fullStr | Aptamer Validation by Western Blot–an overview |
| title_full_unstemmed | Aptamer Validation by Western Blot–an overview |
| title_short | Aptamer Validation by Western Blot–an overview |
| title_sort | aptamer validation by western blot an overview |
| url | https://ajps.uomustansiriyah.edu.iq/index.php/AJPS/article/view/782 |
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