Phenolic Compounds and Antioxidant Capacity Comparison of Wild-Type and Yellow-Leaf <i>gl1</i> Mutant of <i>Lagerstroemia indica</i>
Background: The yellow-leaf <i>gl1</i> mutant of <i>Lagerstroemia indica</i> exhibits an altered phenylpropanoid metabolism pathway compared to wild-type (WT). However, details on the metabolites associated with leaf color variation, including color-specific metabolites with...
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MDPI AG
2024-01-01
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| Online Access: | https://www.mdpi.com/2223-7747/13/2/315 |
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| author | Sumei Li Min Yin Peng Wang Lulu Gao Fenni Lv Rutong Yang Ya Li Qing Wang Linfang Li Yongdong Liu Shuan Wang |
| author_facet | Sumei Li Min Yin Peng Wang Lulu Gao Fenni Lv Rutong Yang Ya Li Qing Wang Linfang Li Yongdong Liu Shuan Wang |
| author_sort | Sumei Li |
| collection | DOAJ |
| description | Background: The yellow-leaf <i>gl1</i> mutant of <i>Lagerstroemia indica</i> exhibits an altered phenylpropanoid metabolism pathway compared to wild-type (WT). However, details on the metabolites associated with leaf color variation, including color-specific metabolites with bioactive constituents, are not fully understood. Methods: Chemical and metabolomics approaches were used to compare metabolite composition and antioxidant capacity between the <i>gl1</i> mutant and WT leaves. Results: The mutant exhibited an irregular xylem structure with a significantly lower phenolic polymer lignin content and higher soluble phenolic compounds. Untargeted metabolomics analysis identified phenolic compounds, particularly lignans, as key differential metabolites between <i>gl1</i> and WT, with a significant increase in the mutant. The neolignan derivative balanophonin-4-<i>O</i>-D-glu was identified as a characteristic metabolite in the <i>gl1</i> mutant. The soluble phenolic compounds of the <i>gl1</i> mutant exhibited higher FRAP, ABTS, DPPH, and hydroxyl radical scavenging activity than in WT. Correlation analysis showed a positive relationship between antioxidant capacity and phenolic compounds in <i>L. indica</i>. Conclusions: Metabolites associated with leaf color variation in the <i>L. indica</i> yellow-leaf <i>gl1</i> mutant demonstrated high antioxidant capacity, particularly in scavenging hydroxyl radicals. |
| first_indexed | 2024-03-08T09:48:16Z |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-08T09:48:16Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-ea4ce90bc71448049b857e3692d8701b2024-01-29T14:12:22ZengMDPI AGPlants2223-77472024-01-0113231510.3390/plants13020315Phenolic Compounds and Antioxidant Capacity Comparison of Wild-Type and Yellow-Leaf <i>gl1</i> Mutant of <i>Lagerstroemia indica</i>Sumei Li0Min Yin1Peng Wang2Lulu Gao3Fenni Lv4Rutong Yang5Ya Li6Qing Wang7Linfang Li8Yongdong Liu9Shuan Wang10Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, No. 1 Qianhu Houcun, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, No. 1 Qianhu Houcun, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, No. 1 Qianhu Houcun, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, No. 1 Qianhu Houcun, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, No. 1 Qianhu Houcun, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, No. 1 Qianhu Houcun, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, No. 1 Qianhu Houcun, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, No. 1 Qianhu Houcun, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, No. 1 Qianhu Houcun, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, No. 1 Qianhu Houcun, Nanjing 210014, ChinaJiangsu Key Laboratory for the Research and Utilization of Plant Resources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Memorial Sun Yat-Sen, No. 1 Qianhu Houcun, Nanjing 210014, ChinaBackground: The yellow-leaf <i>gl1</i> mutant of <i>Lagerstroemia indica</i> exhibits an altered phenylpropanoid metabolism pathway compared to wild-type (WT). However, details on the metabolites associated with leaf color variation, including color-specific metabolites with bioactive constituents, are not fully understood. Methods: Chemical and metabolomics approaches were used to compare metabolite composition and antioxidant capacity between the <i>gl1</i> mutant and WT leaves. Results: The mutant exhibited an irregular xylem structure with a significantly lower phenolic polymer lignin content and higher soluble phenolic compounds. Untargeted metabolomics analysis identified phenolic compounds, particularly lignans, as key differential metabolites between <i>gl1</i> and WT, with a significant increase in the mutant. The neolignan derivative balanophonin-4-<i>O</i>-D-glu was identified as a characteristic metabolite in the <i>gl1</i> mutant. The soluble phenolic compounds of the <i>gl1</i> mutant exhibited higher FRAP, ABTS, DPPH, and hydroxyl radical scavenging activity than in WT. Correlation analysis showed a positive relationship between antioxidant capacity and phenolic compounds in <i>L. indica</i>. Conclusions: Metabolites associated with leaf color variation in the <i>L. indica</i> yellow-leaf <i>gl1</i> mutant demonstrated high antioxidant capacity, particularly in scavenging hydroxyl radicals.https://www.mdpi.com/2223-7747/13/2/315phenolic compoundsantioxidant activitymetabolomicsyellow-leaf mutant<i>Lagerstroemia indica</i> |
| spellingShingle | Sumei Li Min Yin Peng Wang Lulu Gao Fenni Lv Rutong Yang Ya Li Qing Wang Linfang Li Yongdong Liu Shuan Wang Phenolic Compounds and Antioxidant Capacity Comparison of Wild-Type and Yellow-Leaf <i>gl1</i> Mutant of <i>Lagerstroemia indica</i> Plants phenolic compounds antioxidant activity metabolomics yellow-leaf mutant <i>Lagerstroemia indica</i> |
| title | Phenolic Compounds and Antioxidant Capacity Comparison of Wild-Type and Yellow-Leaf <i>gl1</i> Mutant of <i>Lagerstroemia indica</i> |
| title_full | Phenolic Compounds and Antioxidant Capacity Comparison of Wild-Type and Yellow-Leaf <i>gl1</i> Mutant of <i>Lagerstroemia indica</i> |
| title_fullStr | Phenolic Compounds and Antioxidant Capacity Comparison of Wild-Type and Yellow-Leaf <i>gl1</i> Mutant of <i>Lagerstroemia indica</i> |
| title_full_unstemmed | Phenolic Compounds and Antioxidant Capacity Comparison of Wild-Type and Yellow-Leaf <i>gl1</i> Mutant of <i>Lagerstroemia indica</i> |
| title_short | Phenolic Compounds and Antioxidant Capacity Comparison of Wild-Type and Yellow-Leaf <i>gl1</i> Mutant of <i>Lagerstroemia indica</i> |
| title_sort | phenolic compounds and antioxidant capacity comparison of wild type and yellow leaf i gl1 i mutant of i lagerstroemia indica i |
| topic | phenolic compounds antioxidant activity metabolomics yellow-leaf mutant <i>Lagerstroemia indica</i> |
| url | https://www.mdpi.com/2223-7747/13/2/315 |
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