Effects of Nostoc sphaeroids Kütz polysaccharide on renal fibrosis in high‐fat mice
Abstract In this study, we investigated the effects of Nostoc sphaeroids Kütz polysaccharide (NSKP) on renal fibrosis in high‐fat mice. ApoE−/− male mice were randomly divided into four groups: control (Cont) group, high‐fat diet (HFD) group, HFD+0.4 g/kg BW NSKP, and HFD+0.8 g/kg BW NSKP (NSKP grou...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-05-01
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| Series: | Food Science & Nutrition |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/fsn3.2703 |
| _version_ | 1797990681480790016 |
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| author | Litao Yang Yinlu Liu Cuicui Bi Bo Zhang |
| author_facet | Litao Yang Yinlu Liu Cuicui Bi Bo Zhang |
| author_sort | Litao Yang |
| collection | DOAJ |
| description | Abstract In this study, we investigated the effects of Nostoc sphaeroids Kütz polysaccharide (NSKP) on renal fibrosis in high‐fat mice. ApoE−/− male mice were randomly divided into four groups: control (Cont) group, high‐fat diet (HFD) group, HFD+0.4 g/kg BW NSKP, and HFD+0.8 g/kg BW NSKP (NSKP groups). The Cont was fed a standard diet. The HFD group was fed HFD. Every day, NSKP groups were fed HFD, as well as given 0.4 g/kg BW or 0.8 g/kg BW NSKP. After 22 weeks, the serum biochemical indices (TC, TG, LDL‐C, HDL‐C, GLU, BUN, and SCR) were measured. For the kidney, the histopathological sections were observed and analyzed, and inflammatory factors and markers of renal fibrosis were measured. For the NSKP groups, the serum TC, TG, LDL‐C, BUN, and SCR were decreased, HDL‐C significantly increased compared with the HFD group. The protein expressions of TNF‐α, IL‐1β, and TGF‐β1 were significantly downregulated. The α‐SMA in renal cortex was decreased, and the mRNA expression of Col‐I and Col‐IV in renal collagen fibers was downregulated. To sum up, NSKP reduced the blood lipid of HFD mice, downregulated the inflammation of kidney, inhibited the expression of collagen fiber, and improved the renal fibrosis caused by long‐term lipid metabolism disorder. |
| first_indexed | 2024-04-11T08:40:27Z |
| format | Article |
| id | doaj.art-ea512832de164a8f9a660128c9334014 |
| institution | Directory Open Access Journal |
| issn | 2048-7177 |
| language | English |
| last_indexed | 2024-04-11T08:40:27Z |
| publishDate | 2022-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | Food Science & Nutrition |
| spelling | doaj.art-ea512832de164a8f9a660128c93340142022-12-22T04:34:14ZengWileyFood Science & Nutrition2048-71772022-05-011051357136710.1002/fsn3.2703Effects of Nostoc sphaeroids Kütz polysaccharide on renal fibrosis in high‐fat miceLitao Yang0Yinlu Liu1Cuicui Bi2Bo Zhang3Beijing Key Laboratory of Bioactive Substances and Functional Foods Beijing Union University College of Biochemical Engineering Beijing ChinaBeijing Key Laboratory of Bioactive Substances and Functional Foods Beijing Union University College of Biochemical Engineering Beijing ChinaBeijing Key Laboratory of Bioactive Substances and Functional Foods Beijing Union University College of Biochemical Engineering Beijing ChinaBeijing Key Laboratory of Bioactive Substances and Functional Foods Beijing Union University College of Biochemical Engineering Beijing ChinaAbstract In this study, we investigated the effects of Nostoc sphaeroids Kütz polysaccharide (NSKP) on renal fibrosis in high‐fat mice. ApoE−/− male mice were randomly divided into four groups: control (Cont) group, high‐fat diet (HFD) group, HFD+0.4 g/kg BW NSKP, and HFD+0.8 g/kg BW NSKP (NSKP groups). The Cont was fed a standard diet. The HFD group was fed HFD. Every day, NSKP groups were fed HFD, as well as given 0.4 g/kg BW or 0.8 g/kg BW NSKP. After 22 weeks, the serum biochemical indices (TC, TG, LDL‐C, HDL‐C, GLU, BUN, and SCR) were measured. For the kidney, the histopathological sections were observed and analyzed, and inflammatory factors and markers of renal fibrosis were measured. For the NSKP groups, the serum TC, TG, LDL‐C, BUN, and SCR were decreased, HDL‐C significantly increased compared with the HFD group. The protein expressions of TNF‐α, IL‐1β, and TGF‐β1 were significantly downregulated. The α‐SMA in renal cortex was decreased, and the mRNA expression of Col‐I and Col‐IV in renal collagen fibers was downregulated. To sum up, NSKP reduced the blood lipid of HFD mice, downregulated the inflammation of kidney, inhibited the expression of collagen fiber, and improved the renal fibrosis caused by long‐term lipid metabolism disorder.https://doi.org/10.1002/fsn3.2703high‐fat dietinflammationlipid‐decreasingNostoc sphaeroids Kütz polysacchariderenal fibrosis |
| spellingShingle | Litao Yang Yinlu Liu Cuicui Bi Bo Zhang Effects of Nostoc sphaeroids Kütz polysaccharide on renal fibrosis in high‐fat mice Food Science & Nutrition high‐fat diet inflammation lipid‐decreasing Nostoc sphaeroids Kütz polysaccharide renal fibrosis |
| title | Effects of Nostoc sphaeroids Kütz polysaccharide on renal fibrosis in high‐fat mice |
| title_full | Effects of Nostoc sphaeroids Kütz polysaccharide on renal fibrosis in high‐fat mice |
| title_fullStr | Effects of Nostoc sphaeroids Kütz polysaccharide on renal fibrosis in high‐fat mice |
| title_full_unstemmed | Effects of Nostoc sphaeroids Kütz polysaccharide on renal fibrosis in high‐fat mice |
| title_short | Effects of Nostoc sphaeroids Kütz polysaccharide on renal fibrosis in high‐fat mice |
| title_sort | effects of nostoc sphaeroids kutz polysaccharide on renal fibrosis in high fat mice |
| topic | high‐fat diet inflammation lipid‐decreasing Nostoc sphaeroids Kütz polysaccharide renal fibrosis |
| url | https://doi.org/10.1002/fsn3.2703 |
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