Biomechanical, histological, and radiological effects of different phosphodiesterase inhibitors on femoral fracture healing in rats
Purpose: To investigate the biomechanical, histological, and radiological effects of sildenafil and pentoxifylline on femoral fracture healing in rats. Methods: Forty-eight Sprague-Dawley rats were divided into three groups equally according to the pharmacological agents to be investigated. Femoral...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2018-05-01
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| Series: | Journal of Orthopaedic Surgery |
| Online Access: | https://doi.org/10.1177/2309499018777885 |
| _version_ | 1818118006490791936 |
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| author | Yasar Mahsut Dincel Ender Alagoz Yavuz Arikan Aysel Kara Caglar Suzan Cansel Dogru Faruk Ortes Yunus Ziya Arslan |
| author_facet | Yasar Mahsut Dincel Ender Alagoz Yavuz Arikan Aysel Kara Caglar Suzan Cansel Dogru Faruk Ortes Yunus Ziya Arslan |
| author_sort | Yasar Mahsut Dincel |
| collection | DOAJ |
| description | Purpose: To investigate the biomechanical, histological, and radiological effects of sildenafil and pentoxifylline on femoral fracture healing in rats. Methods: Forty-eight Sprague-Dawley rats were divided into three groups equally according to the pharmacological agents to be investigated. Femoral shaft fractures were formed in the left side. Group 1 (control group), group 2, and group 3 were administered with saline, sildenafil, and pentoxifylline during the fracture healing process, respectively. Eight rats from each group were euthanized on days 15 and 30. X-ray images of the rats were taken after euthanasia for radiographical examination. Femur samples were subjected to histopathological and biomechanical (three-point bending) examinations. Results: Radiologically, no difference between the Goldberg scores of the groups was found for day 15 ( p > 0.05), while higher Goldberg scores were obtained from group 2 than that of group 1 ( p > 0.05) and group 3 ( p < 0.05) for day 30. In the biomechanical analysis, higher mean breaking forces were found both for day 15 and day 30 from group 2 than those obtained from group 1 (for day 15 p > 0.05 and day 30 p > 0.05) and group 3 (for day 15 p < 0.05 and day 30 p < 0.01). Higher mean absorbed energy values were obtained from group 2 than those obtained from group 1 (for day 15 p > 0.05 and day 30 p < 0.05) and group 3 (for day 15 p < 0.01 and day 30 p < 0.01). A significant difference was not found between the histological scores of all groups ( p > 0.05) for day 15, while the histological score of group 1 on day 30 was found to be significantly lower than that of sildenafil and pentoxifylline groups ( p < 0.05). Conclusion: Sildenafil had a positive effect on fracture healing, while pentoxifylline did not provide consistent positive effect. |
| first_indexed | 2024-12-11T04:47:26Z |
| format | Article |
| id | doaj.art-ea5383fc813c42aeb5a511bb1e091385 |
| institution | Directory Open Access Journal |
| issn | 2309-4990 |
| language | English |
| last_indexed | 2024-12-11T04:47:26Z |
| publishDate | 2018-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of Orthopaedic Surgery |
| spelling | doaj.art-ea5383fc813c42aeb5a511bb1e0913852022-12-22T01:20:29ZengSAGE PublishingJournal of Orthopaedic Surgery2309-49902018-05-012610.1177/2309499018777885Biomechanical, histological, and radiological effects of different phosphodiesterase inhibitors on femoral fracture healing in ratsYasar Mahsut Dincel0Ender Alagoz1Yavuz Arikan2Aysel Kara Caglar3Suzan Cansel Dogru4Faruk Ortes5Yunus Ziya Arslan6 Department of Orthopaedics and Traumatology, Metin Sabancı Baltalimanı Bone Diseases Training and Research Hospital, Istanbul, Turkey Department of Orthopaedics and Traumatology, İstanbul Bağcılar Training and Research Hospital, Istanbul, Turkey Department of Orthopaedics and Traumatology, Metin Sabancı Baltalimanı Bone Diseases Training and Research Hospital, Istanbul, Turkey Department of Pathology, İstanbul Bağcılar Training and Research Hospital, Istanbul, Turkey Department of Mechanical Engineering, Faculty of Engineering, Istanbul University, Avcilar, Istanbul, Turkey Department of Mechanical Engineering, Faculty of Engineering, Istanbul University, Avcilar, Istanbul, Turkey Department of Mechanical Engineering, Faculty of Engineering, Istanbul University, Avcilar, Istanbul, TurkeyPurpose: To investigate the biomechanical, histological, and radiological effects of sildenafil and pentoxifylline on femoral fracture healing in rats. Methods: Forty-eight Sprague-Dawley rats were divided into three groups equally according to the pharmacological agents to be investigated. Femoral shaft fractures were formed in the left side. Group 1 (control group), group 2, and group 3 were administered with saline, sildenafil, and pentoxifylline during the fracture healing process, respectively. Eight rats from each group were euthanized on days 15 and 30. X-ray images of the rats were taken after euthanasia for radiographical examination. Femur samples were subjected to histopathological and biomechanical (three-point bending) examinations. Results: Radiologically, no difference between the Goldberg scores of the groups was found for day 15 ( p > 0.05), while higher Goldberg scores were obtained from group 2 than that of group 1 ( p > 0.05) and group 3 ( p < 0.05) for day 30. In the biomechanical analysis, higher mean breaking forces were found both for day 15 and day 30 from group 2 than those obtained from group 1 (for day 15 p > 0.05 and day 30 p > 0.05) and group 3 (for day 15 p < 0.05 and day 30 p < 0.01). Higher mean absorbed energy values were obtained from group 2 than those obtained from group 1 (for day 15 p > 0.05 and day 30 p < 0.05) and group 3 (for day 15 p < 0.01 and day 30 p < 0.01). A significant difference was not found between the histological scores of all groups ( p > 0.05) for day 15, while the histological score of group 1 on day 30 was found to be significantly lower than that of sildenafil and pentoxifylline groups ( p < 0.05). Conclusion: Sildenafil had a positive effect on fracture healing, while pentoxifylline did not provide consistent positive effect.https://doi.org/10.1177/2309499018777885 |
| spellingShingle | Yasar Mahsut Dincel Ender Alagoz Yavuz Arikan Aysel Kara Caglar Suzan Cansel Dogru Faruk Ortes Yunus Ziya Arslan Biomechanical, histological, and radiological effects of different phosphodiesterase inhibitors on femoral fracture healing in rats Journal of Orthopaedic Surgery |
| title | Biomechanical, histological, and radiological effects of different phosphodiesterase inhibitors on femoral fracture healing in rats |
| title_full | Biomechanical, histological, and radiological effects of different phosphodiesterase inhibitors on femoral fracture healing in rats |
| title_fullStr | Biomechanical, histological, and radiological effects of different phosphodiesterase inhibitors on femoral fracture healing in rats |
| title_full_unstemmed | Biomechanical, histological, and radiological effects of different phosphodiesterase inhibitors on femoral fracture healing in rats |
| title_short | Biomechanical, histological, and radiological effects of different phosphodiesterase inhibitors on femoral fracture healing in rats |
| title_sort | biomechanical histological and radiological effects of different phosphodiesterase inhibitors on femoral fracture healing in rats |
| url | https://doi.org/10.1177/2309499018777885 |
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