Green synthesis of silver nanoparticles using curcumin: A comparative study of antimicrobial and antibiofilm effects on Acinetobacter baumannii against chemical conventional methods
Acinetobacter baumannii (A. baumannii) is an important pathogen that causes hospital-acquired illnesses worldwide. The biofilm-forming activity of the organism enhances its capacity to acquire significant levels of antibiotic resistance, hence necessitating the development of novel and effective ant...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211715623005131 |
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| author | Marwa A. Abdelwahab Ahmed Nabil Hamza El-Hosainy Rafat Tahway Marwa S. Taha |
| author_facet | Marwa A. Abdelwahab Ahmed Nabil Hamza El-Hosainy Rafat Tahway Marwa S. Taha |
| author_sort | Marwa A. Abdelwahab |
| collection | DOAJ |
| description | Acinetobacter baumannii (A. baumannii) is an important pathogen that causes hospital-acquired illnesses worldwide. The biofilm-forming activity of the organism enhances its capacity to acquire significant levels of antibiotic resistance, hence necessitating the development of novel and effective antibacterial drugs. Nevertheless, nanoparticles have been documented as highly effective agents against bacterial growth and biofilm formation. So, the purpose of this work was to study the antibacterial and antibiofilm capabilities of silver nanoparticles synthesized by facile green method using curcumin (GS-AgNPs) on A. baumannii compared to the chemical method (CS-AgNPs) in vitro. Moreover, twenty-four clinical isolates of A. baumannii were collected and characterized by standard microbiological techniques. The results showed that all isolates of A. baumanii exhibited a significant degree of resistance to a wide range of antimicrobials. On the other hand, the minimum inhibitory concentration (MIC) for GS-AgNPs sample fell between 250 and 7.8 μg/ml. While the CS-AgNPs sample had MIC values that fell between 500 and 1000 µg/ml. Twenty out of 24 strains were biofilm producers. The lowest MIC of the tested AgNPs was mainly observed in the strains that produce weak biofilm. GS-AgNPs significantly inhibited the biofilm production by the tested isolates than AgNPs synthesized chemically and biofilm inhibition reached to 57.43 ± 9.50 %, and 50.57 ± 3.91 % of controls for strong producers, respectively. The results of the research suggest that GS-AgNPs demonstrate notable effectiveness as a secure and powerful antibacterial and antibiofilm agent against multidrug-resistant A. baumannii strains. |
| first_indexed | 2024-03-08T18:43:26Z |
| format | Article |
| id | doaj.art-ea54cafe57ad4e99b2ed43ac9733e3fd |
| institution | Directory Open Access Journal |
| issn | 2211-7156 |
| language | English |
| last_indexed | 2024-03-08T18:43:26Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj.art-ea54cafe57ad4e99b2ed43ac9733e3fd2023-12-29T04:45:16ZengElsevierResults in Chemistry2211-71562024-01-017101274Green synthesis of silver nanoparticles using curcumin: A comparative study of antimicrobial and antibiofilm effects on Acinetobacter baumannii against chemical conventional methodsMarwa A. Abdelwahab0Ahmed Nabil1Hamza El-Hosainy2Rafat Tahway3Marwa S. Taha4Department of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Tanta 31527, EgyptBiotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences (PSAS), Beni-Suef University, Beni-Suef, Egypt; Corresponding authors.Institute of Nanoscience and Nanotechnology, Kafrelsheikh University, Kafrelsheikh 33511, Egypt; Corresponding authors.Central Metallurgical Research and Development Institute, CMRDI, P.O. Box: 87 Helwan, 11421 Cairo, EgyptDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Tanta 31527, EgyptAcinetobacter baumannii (A. baumannii) is an important pathogen that causes hospital-acquired illnesses worldwide. The biofilm-forming activity of the organism enhances its capacity to acquire significant levels of antibiotic resistance, hence necessitating the development of novel and effective antibacterial drugs. Nevertheless, nanoparticles have been documented as highly effective agents against bacterial growth and biofilm formation. So, the purpose of this work was to study the antibacterial and antibiofilm capabilities of silver nanoparticles synthesized by facile green method using curcumin (GS-AgNPs) on A. baumannii compared to the chemical method (CS-AgNPs) in vitro. Moreover, twenty-four clinical isolates of A. baumannii were collected and characterized by standard microbiological techniques. The results showed that all isolates of A. baumanii exhibited a significant degree of resistance to a wide range of antimicrobials. On the other hand, the minimum inhibitory concentration (MIC) for GS-AgNPs sample fell between 250 and 7.8 μg/ml. While the CS-AgNPs sample had MIC values that fell between 500 and 1000 µg/ml. Twenty out of 24 strains were biofilm producers. The lowest MIC of the tested AgNPs was mainly observed in the strains that produce weak biofilm. GS-AgNPs significantly inhibited the biofilm production by the tested isolates than AgNPs synthesized chemically and biofilm inhibition reached to 57.43 ± 9.50 %, and 50.57 ± 3.91 % of controls for strong producers, respectively. The results of the research suggest that GS-AgNPs demonstrate notable effectiveness as a secure and powerful antibacterial and antibiofilm agent against multidrug-resistant A. baumannii strains.http://www.sciencedirect.com/science/article/pii/S2211715623005131Acinetobacter baumanniiSilver NanoparticleCurcuminBiofilmMIC |
| spellingShingle | Marwa A. Abdelwahab Ahmed Nabil Hamza El-Hosainy Rafat Tahway Marwa S. Taha Green synthesis of silver nanoparticles using curcumin: A comparative study of antimicrobial and antibiofilm effects on Acinetobacter baumannii against chemical conventional methods Results in Chemistry Acinetobacter baumannii Silver Nanoparticle Curcumin Biofilm MIC |
| title | Green synthesis of silver nanoparticles using curcumin: A comparative study of antimicrobial and antibiofilm effects on Acinetobacter baumannii against chemical conventional methods |
| title_full | Green synthesis of silver nanoparticles using curcumin: A comparative study of antimicrobial and antibiofilm effects on Acinetobacter baumannii against chemical conventional methods |
| title_fullStr | Green synthesis of silver nanoparticles using curcumin: A comparative study of antimicrobial and antibiofilm effects on Acinetobacter baumannii against chemical conventional methods |
| title_full_unstemmed | Green synthesis of silver nanoparticles using curcumin: A comparative study of antimicrobial and antibiofilm effects on Acinetobacter baumannii against chemical conventional methods |
| title_short | Green synthesis of silver nanoparticles using curcumin: A comparative study of antimicrobial and antibiofilm effects on Acinetobacter baumannii against chemical conventional methods |
| title_sort | green synthesis of silver nanoparticles using curcumin a comparative study of antimicrobial and antibiofilm effects on acinetobacter baumannii against chemical conventional methods |
| topic | Acinetobacter baumannii Silver Nanoparticle Curcumin Biofilm MIC |
| url | http://www.sciencedirect.com/science/article/pii/S2211715623005131 |
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