The Inhibition of Serine/Threonine Protein Phosphatase Type 5 Mediates Cantharidin Toxicity to Control <i>Periplaneta americana</i> (L.)
The American cockroach, <i>Periplaneta americana</i> (L.), is a notorious urban pest. It has developed insecticidal resistance to commonly used insecticides. Cantharidin (CTD) is a defensive toxin derived from blister beetles. It has been verified to have insecticidal toxicity in a range...
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MDPI AG
2020-10-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/10/682 |
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| author | Hong Sun Yifan Li Xinyu Li Yalin Zhang |
| author_facet | Hong Sun Yifan Li Xinyu Li Yalin Zhang |
| author_sort | Hong Sun |
| collection | DOAJ |
| description | The American cockroach, <i>Periplaneta americana</i> (L.), is a notorious urban pest. It has developed insecticidal resistance to commonly used insecticides. Cantharidin (CTD) is a defensive toxin derived from blister beetles. It has been verified to have insecticidal toxicity in a range of pests. In this study, we determined the ingestion toxicity of CTD and norcantharidin (NCTD) to <i>P. americana</i> to test whether they had the potential to be effective against <i>P. americana</i>. Bioassays revealed that CTD produces toxicity against <i>P. americana</i>. The median lethal concentration (LC<sub>50</sub>) value of CTD was 50.92 μg/mL, while NCTD displayed nearly no toxicity against <i>P. americana</i>. The inhibition assays of serine/threonine protein phosphatases (PSPs) in <i>P. americana</i> indicated that CTD and NCTD could inhibit PSPs. The value of the half maximal inhibitory concentration (IC<sub>50</sub>) of CTD was 7.21 ± 0.94 μM, whereas that of NCTD was higher, at 31.65 ± 3.87 μM. Furthermore, the inhibition effect of CTD on the serine/threonine protein phosphatase type 5 of <i>P. americana</i> (PaPP5) was superior to that of NCTD. Specifically, the IC<sub>50</sub> of CTD reached 0.39 ± 0.04 μM, while the IC<sub>50</sub> of NCTD was 1.87 ± 0.23 μM. This study paves the way for insect-derived agents (CTD) to be applied toward controlling <i>P. americana</i> and contributes to the development of novel insecticides based on PP5 as a target. |
| first_indexed | 2024-03-10T15:47:17Z |
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| language | English |
| last_indexed | 2024-03-10T15:47:17Z |
| publishDate | 2020-10-01 |
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| series | Insects |
| spelling | doaj.art-ea5593caaae742519918bbc929dbfe102023-11-20T16:23:19ZengMDPI AGInsects2075-44502020-10-01111068210.3390/insects11100682The Inhibition of Serine/Threonine Protein Phosphatase Type 5 Mediates Cantharidin Toxicity to Control <i>Periplaneta americana</i> (L.)Hong Sun0Yifan Li1Xinyu Li2Yalin Zhang3Key Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, ChinaKey Laboratory of Plant Protection Resources & Pest Management of the Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, ChinaThe American cockroach, <i>Periplaneta americana</i> (L.), is a notorious urban pest. It has developed insecticidal resistance to commonly used insecticides. Cantharidin (CTD) is a defensive toxin derived from blister beetles. It has been verified to have insecticidal toxicity in a range of pests. In this study, we determined the ingestion toxicity of CTD and norcantharidin (NCTD) to <i>P. americana</i> to test whether they had the potential to be effective against <i>P. americana</i>. Bioassays revealed that CTD produces toxicity against <i>P. americana</i>. The median lethal concentration (LC<sub>50</sub>) value of CTD was 50.92 μg/mL, while NCTD displayed nearly no toxicity against <i>P. americana</i>. The inhibition assays of serine/threonine protein phosphatases (PSPs) in <i>P. americana</i> indicated that CTD and NCTD could inhibit PSPs. The value of the half maximal inhibitory concentration (IC<sub>50</sub>) of CTD was 7.21 ± 0.94 μM, whereas that of NCTD was higher, at 31.65 ± 3.87 μM. Furthermore, the inhibition effect of CTD on the serine/threonine protein phosphatase type 5 of <i>P. americana</i> (PaPP5) was superior to that of NCTD. Specifically, the IC<sub>50</sub> of CTD reached 0.39 ± 0.04 μM, while the IC<sub>50</sub> of NCTD was 1.87 ± 0.23 μM. This study paves the way for insect-derived agents (CTD) to be applied toward controlling <i>P. americana</i> and contributes to the development of novel insecticides based on PP5 as a target.https://www.mdpi.com/2075-4450/11/10/682<i>Periplaneta americana</i>Cantharidinserine/threonine protein phosphatase |
| spellingShingle | Hong Sun Yifan Li Xinyu Li Yalin Zhang The Inhibition of Serine/Threonine Protein Phosphatase Type 5 Mediates Cantharidin Toxicity to Control <i>Periplaneta americana</i> (L.) Insects <i>Periplaneta americana</i> Cantharidin serine/threonine protein phosphatase |
| title | The Inhibition of Serine/Threonine Protein Phosphatase Type 5 Mediates Cantharidin Toxicity to Control <i>Periplaneta americana</i> (L.) |
| title_full | The Inhibition of Serine/Threonine Protein Phosphatase Type 5 Mediates Cantharidin Toxicity to Control <i>Periplaneta americana</i> (L.) |
| title_fullStr | The Inhibition of Serine/Threonine Protein Phosphatase Type 5 Mediates Cantharidin Toxicity to Control <i>Periplaneta americana</i> (L.) |
| title_full_unstemmed | The Inhibition of Serine/Threonine Protein Phosphatase Type 5 Mediates Cantharidin Toxicity to Control <i>Periplaneta americana</i> (L.) |
| title_short | The Inhibition of Serine/Threonine Protein Phosphatase Type 5 Mediates Cantharidin Toxicity to Control <i>Periplaneta americana</i> (L.) |
| title_sort | inhibition of serine threonine protein phosphatase type 5 mediates cantharidin toxicity to control i periplaneta americana i l |
| topic | <i>Periplaneta americana</i> Cantharidin serine/threonine protein phosphatase |
| url | https://www.mdpi.com/2075-4450/11/10/682 |
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