Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications
The cancer stem-cell hypothesis proposes that malignant tumors are likely to encompass a cellular hierarchy that parallels normal tissue and may be responsible for the maintenance and recurrence of glioblastoma multiforme (GBM) in patients. The purpose of this manuscript is to review methods for opt...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2011-06-01
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| Series: | Cancers |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2072-6694/3/2/2655/ |
| _version_ | 1797705953138704384 |
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| author | John A. Boockvar Jan-Karl Burkhardt Kartik Kesavabhotla Cody D. Schlaff Demirkan B. Gürsel Benjamin J. Shin |
| author_facet | John A. Boockvar Jan-Karl Burkhardt Kartik Kesavabhotla Cody D. Schlaff Demirkan B. Gürsel Benjamin J. Shin |
| author_sort | John A. Boockvar |
| collection | DOAJ |
| description | The cancer stem-cell hypothesis proposes that malignant tumors are likely to encompass a cellular hierarchy that parallels normal tissue and may be responsible for the maintenance and recurrence of glioblastoma multiforme (GBM) in patients. The purpose of this manuscript is to review methods for optimizing the derivation and culturing of stem-like cells also known as tumor stem cells (TSCs) from patient-derived GBM tissue samples. The hallmarks of TSCs are that they must be able to self-renew and retain tumorigenicity. The isolation, optimization and derivation of TSCs as outlined in this review, will be important in understanding biology and therapeutic applications related to these cells. |
| first_indexed | 2024-03-12T05:43:59Z |
| format | Article |
| id | doaj.art-ea56e253a8e344eda2f80b18a0ec9bea |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-12T05:43:59Z |
| publishDate | 2011-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-ea56e253a8e344eda2f80b18a0ec9bea2023-09-03T05:48:48ZengMDPI AGCancers2072-66942011-06-01322655266610.3390/cancers3022655Glioblastoma Stem-Like Cells—Biology and Therapeutic ImplicationsJohn A. BoockvarJan-Karl BurkhardtKartik KesavabhotlaCody D. SchlaffDemirkan B. GürselBenjamin J. ShinThe cancer stem-cell hypothesis proposes that malignant tumors are likely to encompass a cellular hierarchy that parallels normal tissue and may be responsible for the maintenance and recurrence of glioblastoma multiforme (GBM) in patients. The purpose of this manuscript is to review methods for optimizing the derivation and culturing of stem-like cells also known as tumor stem cells (TSCs) from patient-derived GBM tissue samples. The hallmarks of TSCs are that they must be able to self-renew and retain tumorigenicity. The isolation, optimization and derivation of TSCs as outlined in this review, will be important in understanding biology and therapeutic applications related to these cells.http://www.mdpi.com/2072-6694/3/2/2655/glioblastoma multiformestem-like cellsbrain tumor stem cellsdifferentiationculturingstem-cell hypothesis |
| spellingShingle | John A. Boockvar Jan-Karl Burkhardt Kartik Kesavabhotla Cody D. Schlaff Demirkan B. Gürsel Benjamin J. Shin Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications Cancers glioblastoma multiforme stem-like cells brain tumor stem cells differentiation culturing stem-cell hypothesis |
| title | Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications |
| title_full | Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications |
| title_fullStr | Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications |
| title_full_unstemmed | Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications |
| title_short | Glioblastoma Stem-Like Cells—Biology and Therapeutic Implications |
| title_sort | glioblastoma stem like cells biology and therapeutic implications |
| topic | glioblastoma multiforme stem-like cells brain tumor stem cells differentiation culturing stem-cell hypothesis |
| url | http://www.mdpi.com/2072-6694/3/2/2655/ |
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