Transcriptome and Proteome Co-Profiling Offers an Understanding of Pre-Harvest Sprouting (PHS) Molecular Mechanisms in Wheat (<i>Triticum aestivum</i>)
While wheat (<i>Triticum aestivum</i> L.) is a widely grown and enjoyed crop, the diverse and complex global situation and climate are exacerbating the instability of its supply. In particular, pre-harvest sprouting (PHS) is one of the major abiotic stresses that frequently occurs due to...
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| Format: | Article |
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MDPI AG
2022-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/21/2807 |
| _version_ | 1797466851027976192 |
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| author | Sang Yong Park Woo Joo Jung Geul Bang Heeyoun Hwang Jae Yoon Kim |
| author_facet | Sang Yong Park Woo Joo Jung Geul Bang Heeyoun Hwang Jae Yoon Kim |
| author_sort | Sang Yong Park |
| collection | DOAJ |
| description | While wheat (<i>Triticum aestivum</i> L.) is a widely grown and enjoyed crop, the diverse and complex global situation and climate are exacerbating the instability of its supply. In particular, pre-harvest sprouting (PHS) is one of the major abiotic stresses that frequently occurs due to irregular climate conditions, causing serious damage to wheat and its quality. In this study, transcriptomic analysis with RNA-seq and proteomic analysis with LC-MS/MS were performed in PHS-treated spikes from two wheat cultivars presenting PHS sensitivity and tolerance, respectively. A total of 13,154 differentially expressed genes (DEGs) and 706 differentially expressed proteins (DEPs) were identified in four comparison groups between the susceptible/tolerant cultivars. Gene function and correlation analysis were performed to determine the co-profiled genes and proteins affected by PHS treatment. In the functional annotation of each comparative group, similar functions were confirmed in each cultivar under PHS treatment; however, in Keumgang PHS+7 (K7) vs. Woori PHS+7 (W7), functional annotations presented clear differences in the ”spliceosome” and ”proteasome” pathways. In addition, our results indicate that alternative splicing and ubiquitin–proteasome support the regulation of germination and seed dormancy. This study provides an advanced understanding of the functions involved in transcription and translation related to PHS mechanisms, thus enabling specific proposals for the further analysis of germination and seed dormancy mechanisms and pathways in wheat. |
| first_indexed | 2024-03-09T18:44:32Z |
| format | Article |
| id | doaj.art-ea58e6931145476692a08d0e7224a983 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T18:44:32Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-ea58e6931145476692a08d0e7224a9832023-11-24T06:23:24ZengMDPI AGPlants2223-77472022-10-011121280710.3390/plants11212807Transcriptome and Proteome Co-Profiling Offers an Understanding of Pre-Harvest Sprouting (PHS) Molecular Mechanisms in Wheat (<i>Triticum aestivum</i>)Sang Yong Park0Woo Joo Jung1Geul Bang2Heeyoun Hwang3Jae Yoon Kim4Department of Plant Resources, College of Industrial Science, Kongju National University, Yesan 32439, KoreaInstitute of Life Science and Natural Resources, Korea University, Seoul 02841, KoreaResearch Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, KoreaResearch Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, KoreaDepartment of Plant Resources, College of Industrial Science, Kongju National University, Yesan 32439, KoreaWhile wheat (<i>Triticum aestivum</i> L.) is a widely grown and enjoyed crop, the diverse and complex global situation and climate are exacerbating the instability of its supply. In particular, pre-harvest sprouting (PHS) is one of the major abiotic stresses that frequently occurs due to irregular climate conditions, causing serious damage to wheat and its quality. In this study, transcriptomic analysis with RNA-seq and proteomic analysis with LC-MS/MS were performed in PHS-treated spikes from two wheat cultivars presenting PHS sensitivity and tolerance, respectively. A total of 13,154 differentially expressed genes (DEGs) and 706 differentially expressed proteins (DEPs) were identified in four comparison groups between the susceptible/tolerant cultivars. Gene function and correlation analysis were performed to determine the co-profiled genes and proteins affected by PHS treatment. In the functional annotation of each comparative group, similar functions were confirmed in each cultivar under PHS treatment; however, in Keumgang PHS+7 (K7) vs. Woori PHS+7 (W7), functional annotations presented clear differences in the ”spliceosome” and ”proteasome” pathways. In addition, our results indicate that alternative splicing and ubiquitin–proteasome support the regulation of germination and seed dormancy. This study provides an advanced understanding of the functions involved in transcription and translation related to PHS mechanisms, thus enabling specific proposals for the further analysis of germination and seed dormancy mechanisms and pathways in wheat.https://www.mdpi.com/2223-7747/11/21/2807wheat (<i>Triticum aestivum</i>)abiotic stresspre-harvest sprouting (PHS)RNA-seqtranscriptomeproteome analysis |
| spellingShingle | Sang Yong Park Woo Joo Jung Geul Bang Heeyoun Hwang Jae Yoon Kim Transcriptome and Proteome Co-Profiling Offers an Understanding of Pre-Harvest Sprouting (PHS) Molecular Mechanisms in Wheat (<i>Triticum aestivum</i>) Plants wheat (<i>Triticum aestivum</i>) abiotic stress pre-harvest sprouting (PHS) RNA-seq transcriptome proteome analysis |
| title | Transcriptome and Proteome Co-Profiling Offers an Understanding of Pre-Harvest Sprouting (PHS) Molecular Mechanisms in Wheat (<i>Triticum aestivum</i>) |
| title_full | Transcriptome and Proteome Co-Profiling Offers an Understanding of Pre-Harvest Sprouting (PHS) Molecular Mechanisms in Wheat (<i>Triticum aestivum</i>) |
| title_fullStr | Transcriptome and Proteome Co-Profiling Offers an Understanding of Pre-Harvest Sprouting (PHS) Molecular Mechanisms in Wheat (<i>Triticum aestivum</i>) |
| title_full_unstemmed | Transcriptome and Proteome Co-Profiling Offers an Understanding of Pre-Harvest Sprouting (PHS) Molecular Mechanisms in Wheat (<i>Triticum aestivum</i>) |
| title_short | Transcriptome and Proteome Co-Profiling Offers an Understanding of Pre-Harvest Sprouting (PHS) Molecular Mechanisms in Wheat (<i>Triticum aestivum</i>) |
| title_sort | transcriptome and proteome co profiling offers an understanding of pre harvest sprouting phs molecular mechanisms in wheat i triticum aestivum i |
| topic | wheat (<i>Triticum aestivum</i>) abiotic stress pre-harvest sprouting (PHS) RNA-seq transcriptome proteome analysis |
| url | https://www.mdpi.com/2223-7747/11/21/2807 |
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