Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs
Abstract Background Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis Dogs with B. canis‐induced APR develop dyslipidemia with altered lipoprotein concentration and morpho...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-07-01
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| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.15537 |
| _version_ | 1798030089634447360 |
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| author | Zorana Milanović Jelena Vekić Vladimir Radonjić Anja Ilić Božović Aleksandra Zeljković Jelena Janac Vesna Spasojević‐Kalimanovska Jesse Buch Ramaswamy Chandrashekar Žanka Bojić‐Trbojević Ljiljana Hajduković Mary M. Christopher Milica Kovačević Filipović |
| author_facet | Zorana Milanović Jelena Vekić Vladimir Radonjić Anja Ilić Božović Aleksandra Zeljković Jelena Janac Vesna Spasojević‐Kalimanovska Jesse Buch Ramaswamy Chandrashekar Žanka Bojić‐Trbojević Ljiljana Hajduković Mary M. Christopher Milica Kovačević Filipović |
| author_sort | Zorana Milanović |
| collection | DOAJ |
| description | Abstract Background Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis Dogs with B. canis‐induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals Twenty‐nine client‐owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods Observational cross‐sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA‐1 (radioimmunoassay) and SAA was determined. Results Dogs with B. canis infection had a marked APR (median SAA, 168.3 μg/mL; range, 98.1‐716.2 μg/mL) compared with controls (3.2 μg/mL, 2.0‐4.2 μg/mL) (P < .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89‐7.64 mmol/L versus 6.15 mmol/L, 4.2‐7.4 mmol/L) (P = .02), phospholipid (4.64 mmol/L, 2.6‐6.6 mmol/L versus 5.72 mmol/L, 4.68‐7.0 mmol/L) (P = .02), and α‐lipoproteins (77.5%, 27.7%‐93.5% versus 89.2%, 75.1%‐93.5%) (P = .04), and higher ApoA‐1 (1.36 U, 0.8‐2.56 U versus 0.95 U, 0.73‐1.54 U) concentrations (P = .02). Serum amyloid A correlated with high‐density lipoproteins (HDLs) diameter (rho = .43; P = .03) and ApoA‐1 (rho = .63, P < .001). Conclusions and Clinical Importance Major changes associated with B. canis‐induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA‐1 and SAA concentration increase is a unique still unexplained pathophysiological finding. |
| first_indexed | 2024-04-11T19:35:41Z |
| format | Article |
| id | doaj.art-ea5bbcfda0414d9f89acc6950768da21 |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-04-11T19:35:41Z |
| publishDate | 2019-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-ea5bbcfda0414d9f89acc6950768da212022-12-22T04:06:53ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762019-07-013341686169410.1111/jvim.15537Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogsZorana Milanović0Jelena Vekić1Vladimir Radonjić2Anja Ilić Božović3Aleksandra Zeljković4Jelena Janac5Vesna Spasojević‐Kalimanovska6Jesse Buch7Ramaswamy Chandrashekar8Žanka Bojić‐Trbojević9Ljiljana Hajduković10Mary M. Christopher11Milica Kovačević Filipović12Faculty of Veterinary Medicine University of Belgrade Belgrade SerbiaFaculty of Pharmacy University of Belgrade Belgrade SerbiaSmall animal practice “Petrovac” Beograd SerbiaFaculty of Veterinary Medicine University of Belgrade Belgrade SerbiaFaculty of Pharmacy University of Belgrade Belgrade SerbiaFaculty of Pharmacy University of Belgrade Belgrade SerbiaFaculty of Pharmacy University of Belgrade Belgrade SerbiaIDEXX Laboratories Westbrook MaineIDEXX Laboratories Westbrook MaineINEP ‐ Institute for the Application of Nuclear Energy, University of Belgrade Zemun SerbiaINEP ‐ Institute for the Application of Nuclear Energy, University of Belgrade Zemun SerbiaDepartment of Pathology, Microbiology, and Immunology School of Veterinary Medicine, University of California Davis CaliforniaFaculty of Veterinary Medicine University of Belgrade Belgrade SerbiaAbstract Background Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis Dogs with B. canis‐induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals Twenty‐nine client‐owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods Observational cross‐sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA‐1 (radioimmunoassay) and SAA was determined. Results Dogs with B. canis infection had a marked APR (median SAA, 168.3 μg/mL; range, 98.1‐716.2 μg/mL) compared with controls (3.2 μg/mL, 2.0‐4.2 μg/mL) (P < .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89‐7.64 mmol/L versus 6.15 mmol/L, 4.2‐7.4 mmol/L) (P = .02), phospholipid (4.64 mmol/L, 2.6‐6.6 mmol/L versus 5.72 mmol/L, 4.68‐7.0 mmol/L) (P = .02), and α‐lipoproteins (77.5%, 27.7%‐93.5% versus 89.2%, 75.1%‐93.5%) (P = .04), and higher ApoA‐1 (1.36 U, 0.8‐2.56 U versus 0.95 U, 0.73‐1.54 U) concentrations (P = .02). Serum amyloid A correlated with high‐density lipoproteins (HDLs) diameter (rho = .43; P = .03) and ApoA‐1 (rho = .63, P < .001). Conclusions and Clinical Importance Major changes associated with B. canis‐induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA‐1 and SAA concentration increase is a unique still unexplained pathophysiological finding.https://doi.org/10.1111/jvim.15537acute phase responseapolipoprotein A‐1high‐density lipoproteinlipoprotein diameterserum amyloid A |
| spellingShingle | Zorana Milanović Jelena Vekić Vladimir Radonjić Anja Ilić Božović Aleksandra Zeljković Jelena Janac Vesna Spasojević‐Kalimanovska Jesse Buch Ramaswamy Chandrashekar Žanka Bojić‐Trbojević Ljiljana Hajduković Mary M. Christopher Milica Kovačević Filipović Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs Journal of Veterinary Internal Medicine acute phase response apolipoprotein A‐1 high‐density lipoprotein lipoprotein diameter serum amyloid A |
| title | Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs |
| title_full | Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs |
| title_fullStr | Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs |
| title_full_unstemmed | Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs |
| title_short | Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs |
| title_sort | association of acute babesia canis infection and serum lipid lipoprotein and apoprotein concentrations in dogs |
| topic | acute phase response apolipoprotein A‐1 high‐density lipoprotein lipoprotein diameter serum amyloid A |
| url | https://doi.org/10.1111/jvim.15537 |
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