Anti-Obesity and Gut Microbiota Regulation Effects of Phospholipids from the Eggs of Crab, Portunus Trituberculatus, in High Fat Diet-Fed Mice
There are resourceful phospholipids in the eggs of the crab, <i>Portunus trituberculatus</i> (<i>Pt</i>-PL). However, their components and bioactivities regarding obesity were unclear. Here, we investigated the composition of <i>Pt</i>-PL and their fatty acids. Mo...
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MDPI AG
2022-06-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/20/7/411 |
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| author | Laijin Su Hongli Zhu Sichun Chen Mengyu Du Xiaofeng Wan Yishu Liu Shiwei Hu Yangli Xu |
| author_facet | Laijin Su Hongli Zhu Sichun Chen Mengyu Du Xiaofeng Wan Yishu Liu Shiwei Hu Yangli Xu |
| author_sort | Laijin Su |
| collection | DOAJ |
| description | There are resourceful phospholipids in the eggs of the crab, <i>Portunus trituberculatus</i> (<i>Pt</i>-PL). However, their components and bioactivities regarding obesity were unclear. Here, we investigated the composition of <i>Pt</i>-PL and their fatty acids. Moreover, its effects on obesity and gut microbiota were also evaluated in high fat diet (HFD)-fed mice. The results showed that <i>Pt</i>-PL contained 12 kinds of phospholipids, mainly including phosphatidylcholine (PC, 32.28%), phosphatidylserine (PS, 26.51%), phosphatidic acid (PA, 19.61%), phosphatidylethanolamine (PE, 8.81%), and phosphatidylinositol (PI, 7.96%). Polyunsaturated fatty acids (PUFAs) predominated in the fatty acids components of <i>Pt</i>-PL, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Animal experiments demonstrated that <i>Pt</i>-PL significantly alleviated body weight gain, adipose gain, hepatic gain, fasting blood glucose, serum insulin, lipid levels in serum and the liver, and systematic inflammation in HFD-fed mice. Furthermore, <i>Pt</i>-PL regulated gut microbiota, especially in a dramatic reduction in the ratio of Firmicutes to Bacteroidetes at phylum level, as well as significant amelioration in their subordinate categories. <i>Pt</i>-PL reduced fecal lipopolysaccharide and total bile acids, and elevated fecal short chain fatty acid (SCFA) concentrations, particularly acetate and butyrate. These findings suggest that <i>Pt</i>-PL possesses anti-obesity effects and can alter gut microbiota owing to the abundance of PUFAs. Therefore, <i>Pt</i>-PL may be developed as an effective food supplement for anti-obesity and regulation of human gut health. |
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| language | English |
| last_indexed | 2024-03-09T06:18:15Z |
| publishDate | 2022-06-01 |
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| series | Marine Drugs |
| spelling | doaj.art-ea5e4c0c269e40c28f617e67afe5bcc72023-12-03T11:51:28ZengMDPI AGMarine Drugs1660-33972022-06-0120741110.3390/md20070411Anti-Obesity and Gut Microbiota Regulation Effects of Phospholipids from the Eggs of Crab, Portunus Trituberculatus, in High Fat Diet-Fed MiceLaijin Su0Hongli Zhu1Sichun Chen2Mengyu Du3Xiaofeng Wan4Yishu Liu5Shiwei Hu6Yangli Xu7College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, ChinaNational Engineering Research Center for Maine Aquaculture, Zhejiang Ocean University, Zhoushan 316022, ChinaNational Engineering Research Center for Maine Aquaculture, Zhejiang Ocean University, Zhoushan 316022, ChinaNational Engineering Research Center for Maine Aquaculture, Zhejiang Ocean University, Zhoushan 316022, ChinaNational Engineering Research Center for Maine Aquaculture, Zhejiang Ocean University, Zhoushan 316022, ChinaNational Engineering Research Center for Maine Aquaculture, Zhejiang Ocean University, Zhoushan 316022, ChinaNational Engineering Research Center for Maine Aquaculture, Zhejiang Ocean University, Zhoushan 316022, ChinaWenzhou Academy of Agricultural Science, Wenzhou Characteristic Food Resources Engineering and Technology Research Center, Wenzhou 325006, ChinaThere are resourceful phospholipids in the eggs of the crab, <i>Portunus trituberculatus</i> (<i>Pt</i>-PL). However, their components and bioactivities regarding obesity were unclear. Here, we investigated the composition of <i>Pt</i>-PL and their fatty acids. Moreover, its effects on obesity and gut microbiota were also evaluated in high fat diet (HFD)-fed mice. The results showed that <i>Pt</i>-PL contained 12 kinds of phospholipids, mainly including phosphatidylcholine (PC, 32.28%), phosphatidylserine (PS, 26.51%), phosphatidic acid (PA, 19.61%), phosphatidylethanolamine (PE, 8.81%), and phosphatidylinositol (PI, 7.96%). Polyunsaturated fatty acids (PUFAs) predominated in the fatty acids components of <i>Pt</i>-PL, especially eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Animal experiments demonstrated that <i>Pt</i>-PL significantly alleviated body weight gain, adipose gain, hepatic gain, fasting blood glucose, serum insulin, lipid levels in serum and the liver, and systematic inflammation in HFD-fed mice. Furthermore, <i>Pt</i>-PL regulated gut microbiota, especially in a dramatic reduction in the ratio of Firmicutes to Bacteroidetes at phylum level, as well as significant amelioration in their subordinate categories. <i>Pt</i>-PL reduced fecal lipopolysaccharide and total bile acids, and elevated fecal short chain fatty acid (SCFA) concentrations, particularly acetate and butyrate. These findings suggest that <i>Pt</i>-PL possesses anti-obesity effects and can alter gut microbiota owing to the abundance of PUFAs. Therefore, <i>Pt</i>-PL may be developed as an effective food supplement for anti-obesity and regulation of human gut health.https://www.mdpi.com/1660-3397/20/7/411phospholipidscomposition of fatty acids<i>Portunus trituberculatus</i> eggsanti-obesitygut microbiota |
| spellingShingle | Laijin Su Hongli Zhu Sichun Chen Mengyu Du Xiaofeng Wan Yishu Liu Shiwei Hu Yangli Xu Anti-Obesity and Gut Microbiota Regulation Effects of Phospholipids from the Eggs of Crab, Portunus Trituberculatus, in High Fat Diet-Fed Mice Marine Drugs phospholipids composition of fatty acids <i>Portunus trituberculatus</i> eggs anti-obesity gut microbiota |
| title | Anti-Obesity and Gut Microbiota Regulation Effects of Phospholipids from the Eggs of Crab, Portunus Trituberculatus, in High Fat Diet-Fed Mice |
| title_full | Anti-Obesity and Gut Microbiota Regulation Effects of Phospholipids from the Eggs of Crab, Portunus Trituberculatus, in High Fat Diet-Fed Mice |
| title_fullStr | Anti-Obesity and Gut Microbiota Regulation Effects of Phospholipids from the Eggs of Crab, Portunus Trituberculatus, in High Fat Diet-Fed Mice |
| title_full_unstemmed | Anti-Obesity and Gut Microbiota Regulation Effects of Phospholipids from the Eggs of Crab, Portunus Trituberculatus, in High Fat Diet-Fed Mice |
| title_short | Anti-Obesity and Gut Microbiota Regulation Effects of Phospholipids from the Eggs of Crab, Portunus Trituberculatus, in High Fat Diet-Fed Mice |
| title_sort | anti obesity and gut microbiota regulation effects of phospholipids from the eggs of crab portunus trituberculatus in high fat diet fed mice |
| topic | phospholipids composition of fatty acids <i>Portunus trituberculatus</i> eggs anti-obesity gut microbiota |
| url | https://www.mdpi.com/1660-3397/20/7/411 |
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