A Hand-Held Platform for Boar Sperm Viability Diagnosis Based on Smartphone

The swine fever virus seriously affects pork production, and to improve pork production, pig breeding efficiency needs to be improved, and the detection of boar sperm activity is an important part of the pig breeding process. Traditional laboratory testing methods rely on bulky testing equipment, su...

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Main Authors: Yunhong Zheng, Hang Yin, Chengxian Zhou, Wei Zhou, Zhijie Huan, Weicheng Ma
Format: Article
Language:English
Published: MDPI AG 2023-11-01
Series:Biosensors
Subjects:
Online Access:https://www.mdpi.com/2079-6374/13/11/978
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author Yunhong Zheng
Hang Yin
Chengxian Zhou
Wei Zhou
Zhijie Huan
Weicheng Ma
author_facet Yunhong Zheng
Hang Yin
Chengxian Zhou
Wei Zhou
Zhijie Huan
Weicheng Ma
author_sort Yunhong Zheng
collection DOAJ
description The swine fever virus seriously affects pork production, and to improve pork production, pig breeding efficiency needs to be improved, and the detection of boar sperm activity is an important part of the pig breeding process. Traditional laboratory testing methods rely on bulky testing equipment, such as phase-contrast microscopes, high-speed cameras, and computers, which limit the testing scenarios. To solve the above problems, in this paper, a microfluidic chip was designed to simulate sperm in the oviduct with a channel thickness of 20 um, which can only accommodate sperm for two-dimensional movement. A miniature microscope system which can be used in combination with a smartphone is designed that is only the size of the palm of the hand and has a magnification of about 38 times. An intelligent diagnostic app was developed using Java language, which can automatically identify and track boar sperm with a recognition rate of 96.08% and an average tracking rate of 86%. The results show that the proposed smartphone-based hand-held platform can effectively replace the traditional microscope compound computer to diagnose sperm activity. In contrast, the platform is smaller, easier to use and is not limited by the usage scenarios.
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spelling doaj.art-ea5e95396a25470bb03a78ee22b0d1e42023-11-24T14:32:24ZengMDPI AGBiosensors2079-63742023-11-01131197810.3390/bios13110978A Hand-Held Platform for Boar Sperm Viability Diagnosis Based on SmartphoneYunhong Zheng0Hang Yin1Chengxian Zhou2Wei Zhou3Zhijie Huan4Weicheng Ma5School of Electrical Engineering and Automation, Xiamen University of Technology, Xiamen 361024, ChinaSchool of Electrical and Information Engineering, Jiangsu University, Zhenjiang 212013, ChinaSchool of Electrical Engineering and Automation, Xiamen University of Technology, Xiamen 361024, ChinaSchool of Electrical Engineering and Automation, Xiamen University of Technology, Xiamen 361024, ChinaSchool of Electrical Engineering and Automation, Xiamen University of Technology, Xiamen 361024, ChinaSchool of Electrical Engineering and Automation, Xiamen University of Technology, Xiamen 361024, ChinaThe swine fever virus seriously affects pork production, and to improve pork production, pig breeding efficiency needs to be improved, and the detection of boar sperm activity is an important part of the pig breeding process. Traditional laboratory testing methods rely on bulky testing equipment, such as phase-contrast microscopes, high-speed cameras, and computers, which limit the testing scenarios. To solve the above problems, in this paper, a microfluidic chip was designed to simulate sperm in the oviduct with a channel thickness of 20 um, which can only accommodate sperm for two-dimensional movement. A miniature microscope system which can be used in combination with a smartphone is designed that is only the size of the palm of the hand and has a magnification of about 38 times. An intelligent diagnostic app was developed using Java language, which can automatically identify and track boar sperm with a recognition rate of 96.08% and an average tracking rate of 86%. The results show that the proposed smartphone-based hand-held platform can effectively replace the traditional microscope compound computer to diagnose sperm activity. In contrast, the platform is smaller, easier to use and is not limited by the usage scenarios.https://www.mdpi.com/2079-6374/13/11/978sperm motilityimage processingminiature microscopemicrofluidic chipsmartphone
spellingShingle Yunhong Zheng
Hang Yin
Chengxian Zhou
Wei Zhou
Zhijie Huan
Weicheng Ma
A Hand-Held Platform for Boar Sperm Viability Diagnosis Based on Smartphone
Biosensors
sperm motility
image processing
miniature microscope
microfluidic chip
smartphone
title A Hand-Held Platform for Boar Sperm Viability Diagnosis Based on Smartphone
title_full A Hand-Held Platform for Boar Sperm Viability Diagnosis Based on Smartphone
title_fullStr A Hand-Held Platform for Boar Sperm Viability Diagnosis Based on Smartphone
title_full_unstemmed A Hand-Held Platform for Boar Sperm Viability Diagnosis Based on Smartphone
title_short A Hand-Held Platform for Boar Sperm Viability Diagnosis Based on Smartphone
title_sort hand held platform for boar sperm viability diagnosis based on smartphone
topic sperm motility
image processing
miniature microscope
microfluidic chip
smartphone
url https://www.mdpi.com/2079-6374/13/11/978
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