Composition of <i>Polygonatum zanlanscianense</i> Pamp. Steam and Leaf Phenolic Extract and Its Protective Mechanism on t-BHP-Induced Oxidative Damage of HepG2 Cells

Plant phenolic compounds have attracted considerable attention because of their health benefits. This study aimed to investigate the composition and antioxidant activity of phenol extracts from <i>Polygonatum zanlanscianense</i> Pamp. steam and leaf (PPP). The FTIR, UPLC-Q-Obtrip-MS, and...

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Main Authors: Shuang Tang, Jin Yong, Jin Yan, Teng Peng, Fei Long, Hulan Chen
Format: Article
Language:English
Published: MDPI AG 2023-11-01
Series:Molecules
Subjects:
Online Access:https://www.mdpi.com/1420-3049/28/22/7487
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author Shuang Tang
Jin Yong
Jin Yan
Teng Peng
Fei Long
Hulan Chen
author_facet Shuang Tang
Jin Yong
Jin Yan
Teng Peng
Fei Long
Hulan Chen
author_sort Shuang Tang
collection DOAJ
description Plant phenolic compounds have attracted considerable attention because of their health benefits. This study aimed to investigate the composition and antioxidant activity of phenol extracts from <i>Polygonatum zanlanscianense</i> Pamp. steam and leaf (PPP). The FTIR, UPLC-Q-Obtrip-MS, and HPLC−DAD methods were used to analyze the composition of PPP, and 20 phenolic compounds were preliminarily identified. Among them, the contents of hyperin, astragalin, and diosmetin levels were the highest. Treatment with PPP can significantly reduce t-BHP-induced cell damage in HepG2 cells, reactive oxygen species (ROS) accumulation, and malondialdehyde (MDA) content. Meanwhile, the superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH−Px) activities can be increased. Moreover, PPP enhanced Nrf2 expression, which was consistent with that of heme oxygenase-1 (HO−1), glutamate–cysteine ligase catalytic subunit (GCLC), and NAD(P)H quinone oxidoreductase-1 (NQO1), whereas the expression of Keap1, the Nrf2 inhibitor, was decreased. All findings indicate that PPP can serve as a natural bioactive substance for preventing oxidative stress.
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spelling doaj.art-ea5f310758b54a25aa43cfd778341b002023-11-24T14:57:54ZengMDPI AGMolecules1420-30492023-11-012822748710.3390/molecules28227487Composition of <i>Polygonatum zanlanscianense</i> Pamp. Steam and Leaf Phenolic Extract and Its Protective Mechanism on t-BHP-Induced Oxidative Damage of HepG2 CellsShuang Tang0Jin Yong1Jin Yan2Teng Peng3Fei Long4Hulan Chen5School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaSchool of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, ChinaPlant phenolic compounds have attracted considerable attention because of their health benefits. This study aimed to investigate the composition and antioxidant activity of phenol extracts from <i>Polygonatum zanlanscianense</i> Pamp. steam and leaf (PPP). The FTIR, UPLC-Q-Obtrip-MS, and HPLC−DAD methods were used to analyze the composition of PPP, and 20 phenolic compounds were preliminarily identified. Among them, the contents of hyperin, astragalin, and diosmetin levels were the highest. Treatment with PPP can significantly reduce t-BHP-induced cell damage in HepG2 cells, reactive oxygen species (ROS) accumulation, and malondialdehyde (MDA) content. Meanwhile, the superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH−Px) activities can be increased. Moreover, PPP enhanced Nrf2 expression, which was consistent with that of heme oxygenase-1 (HO−1), glutamate–cysteine ligase catalytic subunit (GCLC), and NAD(P)H quinone oxidoreductase-1 (NQO1), whereas the expression of Keap1, the Nrf2 inhibitor, was decreased. All findings indicate that PPP can serve as a natural bioactive substance for preventing oxidative stress.https://www.mdpi.com/1420-3049/28/22/7487antioxidant activityphenol<i>Polygonatum zanlanscianense</i> Pamp.steam and leafoxidative stressUPLC-Q-Obtrip-MS
spellingShingle Shuang Tang
Jin Yong
Jin Yan
Teng Peng
Fei Long
Hulan Chen
Composition of <i>Polygonatum zanlanscianense</i> Pamp. Steam and Leaf Phenolic Extract and Its Protective Mechanism on t-BHP-Induced Oxidative Damage of HepG2 Cells
Molecules
antioxidant activity
phenol
<i>Polygonatum zanlanscianense</i> Pamp.
steam and leaf
oxidative stress
UPLC-Q-Obtrip-MS
title Composition of <i>Polygonatum zanlanscianense</i> Pamp. Steam and Leaf Phenolic Extract and Its Protective Mechanism on t-BHP-Induced Oxidative Damage of HepG2 Cells
title_full Composition of <i>Polygonatum zanlanscianense</i> Pamp. Steam and Leaf Phenolic Extract and Its Protective Mechanism on t-BHP-Induced Oxidative Damage of HepG2 Cells
title_fullStr Composition of <i>Polygonatum zanlanscianense</i> Pamp. Steam and Leaf Phenolic Extract and Its Protective Mechanism on t-BHP-Induced Oxidative Damage of HepG2 Cells
title_full_unstemmed Composition of <i>Polygonatum zanlanscianense</i> Pamp. Steam and Leaf Phenolic Extract and Its Protective Mechanism on t-BHP-Induced Oxidative Damage of HepG2 Cells
title_short Composition of <i>Polygonatum zanlanscianense</i> Pamp. Steam and Leaf Phenolic Extract and Its Protective Mechanism on t-BHP-Induced Oxidative Damage of HepG2 Cells
title_sort composition of i polygonatum zanlanscianense i pamp steam and leaf phenolic extract and its protective mechanism on t bhp induced oxidative damage of hepg2 cells
topic antioxidant activity
phenol
<i>Polygonatum zanlanscianense</i> Pamp.
steam and leaf
oxidative stress
UPLC-Q-Obtrip-MS
url https://www.mdpi.com/1420-3049/28/22/7487
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