PARIS, an optogenetic method for functionally mapping gap junctions
Cell-cell communication via gap junctions regulates a wide range of physiological processes by enabling the direct intercellular electrical and chemical coupling. However, the in vivo distribution and function of gap junctions remain poorly understood, partly due to the lack of non-invasive tools wi...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2019-01-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/43366 |
| _version_ | 1811253037966032896 |
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| author | Ling Wu Ao Dong Liting Dong Shi-Qiang Wang Yulong Li |
| author_facet | Ling Wu Ao Dong Liting Dong Shi-Qiang Wang Yulong Li |
| author_sort | Ling Wu |
| collection | DOAJ |
| description | Cell-cell communication via gap junctions regulates a wide range of physiological processes by enabling the direct intercellular electrical and chemical coupling. However, the in vivo distribution and function of gap junctions remain poorly understood, partly due to the lack of non-invasive tools with both cell-type specificity and high spatiotemporal resolution. Here, we developed PARIS (pairing actuators and receivers to optically isolate gap junctions), a new fully genetically encoded tool for measuring the cell-specific gap junctional coupling (GJC). PARIS successfully enabled monitoring of GJC in several cultured cell lines under physiologically relevant conditions and in distinct genetically defined neurons in Drosophila brain, with ~10 s temporal resolution and sub-cellular spatial resolution. These results demonstrate that PARIS is a robust, highly sensitive tool for mapping functional gap junctions and study their regulation in both health and disease. |
| first_indexed | 2024-04-12T16:44:43Z |
| format | Article |
| id | doaj.art-ea60ce3a8da04069959124f4ae10121b |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:44:43Z |
| publishDate | 2019-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-ea60ce3a8da04069959124f4ae10121b2022-12-22T03:24:39ZengeLife Sciences Publications LtdeLife2050-084X2019-01-01810.7554/eLife.43366PARIS, an optogenetic method for functionally mapping gap junctionsLing Wu0https://orcid.org/0000-0003-3921-5626Ao Dong1https://orcid.org/0000-0002-2821-9528Liting Dong2https://orcid.org/0000-0001-8396-374XShi-Qiang Wang3Yulong Li4https://orcid.org/0000-0002-9166-9919State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China; PKU-IDG/McGovern Institute for Brain Research, Beijing, China; Peking-Tsinghua Center for Life Sciences, Beijing, ChinaState Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China; PKU-IDG/McGovern Institute for Brain Research, Beijing, China; Peking-Tsinghua Center for Life Sciences, Beijing, ChinaPeking-Tsinghua Center for Life Sciences, Beijing, ChinaState Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, ChinaState Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China; PKU-IDG/McGovern Institute for Brain Research, Beijing, China; Peking-Tsinghua Center for Life Sciences, Beijing, China; Chinese Institute for Brain Research, Beijing, ChinaCell-cell communication via gap junctions regulates a wide range of physiological processes by enabling the direct intercellular electrical and chemical coupling. However, the in vivo distribution and function of gap junctions remain poorly understood, partly due to the lack of non-invasive tools with both cell-type specificity and high spatiotemporal resolution. Here, we developed PARIS (pairing actuators and receivers to optically isolate gap junctions), a new fully genetically encoded tool for measuring the cell-specific gap junctional coupling (GJC). PARIS successfully enabled monitoring of GJC in several cultured cell lines under physiologically relevant conditions and in distinct genetically defined neurons in Drosophila brain, with ~10 s temporal resolution and sub-cellular spatial resolution. These results demonstrate that PARIS is a robust, highly sensitive tool for mapping functional gap junctions and study their regulation in both health and disease.https://elifesciences.org/articles/43366optogeneticsgap junctionscardiomyocyteselectrical synapsesolfactory system |
| spellingShingle | Ling Wu Ao Dong Liting Dong Shi-Qiang Wang Yulong Li PARIS, an optogenetic method for functionally mapping gap junctions eLife optogenetics gap junctions cardiomyocytes electrical synapses olfactory system |
| title | PARIS, an optogenetic method for functionally mapping gap junctions |
| title_full | PARIS, an optogenetic method for functionally mapping gap junctions |
| title_fullStr | PARIS, an optogenetic method for functionally mapping gap junctions |
| title_full_unstemmed | PARIS, an optogenetic method for functionally mapping gap junctions |
| title_short | PARIS, an optogenetic method for functionally mapping gap junctions |
| title_sort | paris an optogenetic method for functionally mapping gap junctions |
| topic | optogenetics gap junctions cardiomyocytes electrical synapses olfactory system |
| url | https://elifesciences.org/articles/43366 |
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