Apolipophorin-II/I Contributes to Cuticular Hydrocarbon Transport and Cuticle Barrier Construction in Locusta migratoria
Apolipophorins are carrier proteins that bind lipids and mediate their transport from tissue to tissue in animals. Apolipophorin I and II (apoLp-II/I) are the major apolipophorins in insects. The implication of apoLp-II/I in cuticle lipid-barrier formation in insects has not been addressed to date....
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.00790/full |
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| author | Yiyan Zhao Yiyan Zhao Weimin Liu Xiaoming Zhao Zhitao Yu Hongfang Guo Hongfang Guo Yang Yang Yang Yang Jianqin Zhang Bernard Moussian Bernard Moussian Jianzhen Zhang |
| author_facet | Yiyan Zhao Yiyan Zhao Weimin Liu Xiaoming Zhao Zhitao Yu Hongfang Guo Hongfang Guo Yang Yang Yang Yang Jianqin Zhang Bernard Moussian Bernard Moussian Jianzhen Zhang |
| author_sort | Yiyan Zhao |
| collection | DOAJ |
| description | Apolipophorins are carrier proteins that bind lipids and mediate their transport from tissue to tissue in animals. Apolipophorin I and II (apoLp-II/I) are the major apolipophorins in insects. The implication of apoLp-II/I in cuticle lipid-barrier formation in insects has not been addressed to date. In the present study, we investigated the function of apoLp-II/I in the migratory locust Locusta migratoria (LmapoLp-II/I). During the development of fifth instar nymphs, LmapoLp-II/I transcript levels increased until mid-instar, and then decreased gradually until molting to the adult stage. We found that LmapoLp-II/I was predominately expressed in the fat body and the integument including oenocytes and epidermal cells. Immunodetection experiments revealed that LmapoLp-I mainly localized in the cytoplasm of oenocytes and epidermal cells. Silencing of LmapoLp-II/I caused molting defects in nymphs. Importantly, RNA interference against LmapoLp-II/I resulted in a significant decrease in the content of cuticle surface lipids including alkanes and methyl alkanes. Cuticular permeability was significantly enhanced in these nymphs in Eosin Y penetration assays. By consequence, desiccation resistance and insecticide tolerance of dsLmapoLp-II/I-treated locusts were reduced. Taken together, our results indicate that LmapoLp-II/I is involved in the transport and deposition of surface-cuticular lipids that are crucial for maintaining normal cuticle barrier function in L. migratoria. |
| first_indexed | 2024-12-12T09:28:41Z |
| format | Article |
| id | doaj.art-ea61dbfdfa1945c995d57a8cf1ed2844 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-12T09:28:41Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-ea61dbfdfa1945c995d57a8cf1ed28442022-12-22T00:28:57ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-07-011110.3389/fphys.2020.00790544382Apolipophorin-II/I Contributes to Cuticular Hydrocarbon Transport and Cuticle Barrier Construction in Locusta migratoriaYiyan Zhao0Yiyan Zhao1Weimin Liu2Xiaoming Zhao3Zhitao Yu4Hongfang Guo5Hongfang Guo6Yang Yang7Yang Yang8Jianqin Zhang9Bernard Moussian10Bernard Moussian11Jianzhen Zhang12Research Institute of Applied Biology, Shanxi University, Taiyuan, ChinaCollege of Life Science, Shanxi University, Taiyuan, ChinaResearch Institute of Applied Biology, Shanxi University, Taiyuan, ChinaResearch Institute of Applied Biology, Shanxi University, Taiyuan, ChinaResearch Institute of Applied Biology, Shanxi University, Taiyuan, ChinaResearch Institute of Applied Biology, Shanxi University, Taiyuan, ChinaCollege of Life Science, Shanxi University, Taiyuan, ChinaResearch Institute of Applied Biology, Shanxi University, Taiyuan, ChinaCollege of Life Science, Shanxi University, Taiyuan, ChinaModern Research Center for Traditional Chinese Medicine, Shanxi University, Taiyuan, ChinaInterfaculty Institute of Cell Biology, University of Tübingen, Tübingen, GermanyUniversité Côte d’Azur, CNRS, Inserm, iBV, Nice, FranceResearch Institute of Applied Biology, Shanxi University, Taiyuan, ChinaApolipophorins are carrier proteins that bind lipids and mediate their transport from tissue to tissue in animals. Apolipophorin I and II (apoLp-II/I) are the major apolipophorins in insects. The implication of apoLp-II/I in cuticle lipid-barrier formation in insects has not been addressed to date. In the present study, we investigated the function of apoLp-II/I in the migratory locust Locusta migratoria (LmapoLp-II/I). During the development of fifth instar nymphs, LmapoLp-II/I transcript levels increased until mid-instar, and then decreased gradually until molting to the adult stage. We found that LmapoLp-II/I was predominately expressed in the fat body and the integument including oenocytes and epidermal cells. Immunodetection experiments revealed that LmapoLp-I mainly localized in the cytoplasm of oenocytes and epidermal cells. Silencing of LmapoLp-II/I caused molting defects in nymphs. Importantly, RNA interference against LmapoLp-II/I resulted in a significant decrease in the content of cuticle surface lipids including alkanes and methyl alkanes. Cuticular permeability was significantly enhanced in these nymphs in Eosin Y penetration assays. By consequence, desiccation resistance and insecticide tolerance of dsLmapoLp-II/I-treated locusts were reduced. Taken together, our results indicate that LmapoLp-II/I is involved in the transport and deposition of surface-cuticular lipids that are crucial for maintaining normal cuticle barrier function in L. migratoria.https://www.frontiersin.org/article/10.3389/fphys.2020.00790/fullLocusta migratoriacuticular lipidsapolipophorincuticular hydrocarbonsRNAi |
| spellingShingle | Yiyan Zhao Yiyan Zhao Weimin Liu Xiaoming Zhao Zhitao Yu Hongfang Guo Hongfang Guo Yang Yang Yang Yang Jianqin Zhang Bernard Moussian Bernard Moussian Jianzhen Zhang Apolipophorin-II/I Contributes to Cuticular Hydrocarbon Transport and Cuticle Barrier Construction in Locusta migratoria Frontiers in Physiology Locusta migratoria cuticular lipids apolipophorin cuticular hydrocarbons RNAi |
| title | Apolipophorin-II/I Contributes to Cuticular Hydrocarbon Transport and Cuticle Barrier Construction in Locusta migratoria |
| title_full | Apolipophorin-II/I Contributes to Cuticular Hydrocarbon Transport and Cuticle Barrier Construction in Locusta migratoria |
| title_fullStr | Apolipophorin-II/I Contributes to Cuticular Hydrocarbon Transport and Cuticle Barrier Construction in Locusta migratoria |
| title_full_unstemmed | Apolipophorin-II/I Contributes to Cuticular Hydrocarbon Transport and Cuticle Barrier Construction in Locusta migratoria |
| title_short | Apolipophorin-II/I Contributes to Cuticular Hydrocarbon Transport and Cuticle Barrier Construction in Locusta migratoria |
| title_sort | apolipophorin ii i contributes to cuticular hydrocarbon transport and cuticle barrier construction in locusta migratoria |
| topic | Locusta migratoria cuticular lipids apolipophorin cuticular hydrocarbons RNAi |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.00790/full |
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