Clostridium difficile in ready-to-eat foods in Isfahan and Shahrekord, Iran
Objective:: To determine the prevalence and antimicrobial resistance of Clostridium difficile (C. difficile) isolated from ready-to-eat foods of Iran. Methods:: From January to August 2013, a total of 368 unpacked ready-to-eat food samples were purchased from randomly selected supermarkets, retail s...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-02-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169115301568 |
| _version_ | 1819206654198022144 |
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| author | Ebrahim Rahimi Zahra Sadat Afzali Zeinab Torki Baghbadorani |
| author_facet | Ebrahim Rahimi Zahra Sadat Afzali Zeinab Torki Baghbadorani |
| author_sort | Ebrahim Rahimi |
| collection | DOAJ |
| description | Objective:: To determine the prevalence and antimicrobial resistance of Clostridium difficile (C. difficile) isolated from ready-to-eat foods of Iran.
Methods:: From January to August 2013, a total of 368 unpacked ready-to-eat food samples were purchased from randomly selected supermarkets, retail stores and restaurants located in Isfahan and Shahrekord, Iran and were evaluated for the presence of C. difficile.
Results:: C. difficile spores were detected in 5 (1.36%) of the 368 samples. The highest prevalence of C. difficile was found in fasl salad (4.29%), followed by yogurt stew (2%), and olovyeh salad (0.93%). All 140 maccaroni salad and falafel sandwich samples were negative for C. difficile. One of the five C. difficile isolates (20%) contained tcdA, tcdB and cdtB toxin genes and four strains (80%) contained tcdA, and tcdB toxin genes. Also, among the five C. difficile isolates, only three strains were found to be toxigenic for toxin A and/or B by ELISA. Isolates were susceptible to vancomycin and metronidazole, but variably resistant to other antimicrobial drugs.
Conclusions:: This study, combined with studies on other food sources, suggests that widespread contamination of food is common. |
| first_indexed | 2024-12-23T05:11:02Z |
| format | Article |
| id | doaj.art-ea63264461c14d30ab320bb7392ae709 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-23T05:11:02Z |
| publishDate | 2015-02-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-ea63264461c14d30ab320bb7392ae7092022-12-21T17:58:58ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912015-02-015212813110.1016/S2221-1691(15)30156-8Clostridium difficile in ready-to-eat foods in Isfahan and Shahrekord, IranEbrahim Rahimi0Zahra Sadat Afzali1Zeinab Torki Baghbadorani2Department of Food Hygiene, College of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, IranDepartment of Biology, College of Basic Science, Shahrekord Branch, Islamic Azad University, Shahrekord, IranDepartment of Food Science and Technology, College of Agriculture, Shahrekord Branch, Islamic Azad University, Shahrekord, IranObjective:: To determine the prevalence and antimicrobial resistance of Clostridium difficile (C. difficile) isolated from ready-to-eat foods of Iran. Methods:: From January to August 2013, a total of 368 unpacked ready-to-eat food samples were purchased from randomly selected supermarkets, retail stores and restaurants located in Isfahan and Shahrekord, Iran and were evaluated for the presence of C. difficile. Results:: C. difficile spores were detected in 5 (1.36%) of the 368 samples. The highest prevalence of C. difficile was found in fasl salad (4.29%), followed by yogurt stew (2%), and olovyeh salad (0.93%). All 140 maccaroni salad and falafel sandwich samples were negative for C. difficile. One of the five C. difficile isolates (20%) contained tcdA, tcdB and cdtB toxin genes and four strains (80%) contained tcdA, and tcdB toxin genes. Also, among the five C. difficile isolates, only three strains were found to be toxigenic for toxin A and/or B by ELISA. Isolates were susceptible to vancomycin and metronidazole, but variably resistant to other antimicrobial drugs. Conclusions:: This study, combined with studies on other food sources, suggests that widespread contamination of food is common.http://www.sciencedirect.com/science/article/pii/S2221169115301568Clostridium difficileReady-to-eat foodsELISA |
| spellingShingle | Ebrahim Rahimi Zahra Sadat Afzali Zeinab Torki Baghbadorani Clostridium difficile in ready-to-eat foods in Isfahan and Shahrekord, Iran Asian Pacific Journal of Tropical Biomedicine Clostridium difficile Ready-to-eat foods ELISA |
| title | Clostridium difficile in ready-to-eat foods in Isfahan and Shahrekord, Iran |
| title_full | Clostridium difficile in ready-to-eat foods in Isfahan and Shahrekord, Iran |
| title_fullStr | Clostridium difficile in ready-to-eat foods in Isfahan and Shahrekord, Iran |
| title_full_unstemmed | Clostridium difficile in ready-to-eat foods in Isfahan and Shahrekord, Iran |
| title_short | Clostridium difficile in ready-to-eat foods in Isfahan and Shahrekord, Iran |
| title_sort | clostridium difficile in ready to eat foods in isfahan and shahrekord iran |
| topic | Clostridium difficile Ready-to-eat foods ELISA |
| url | http://www.sciencedirect.com/science/article/pii/S2221169115301568 |
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