The Juvenile-Hormone-Responsive Factor <i>AmKr-h1</i> Regulates Caste Differentiation in Honey Bees
Honey bees are typical model organisms for the study of caste differentiation, and the juvenile hormone (JH) is a crucial link in the regulatory network of caste differentiation in honey bees. To investigate the mechanism of JH-mediated caste differentiation, we analyzed the effect of the JH respons...
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MDPI AG
2023-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/11/1657 |
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| author | Zhi-Xian Gong Fu-Ping Cheng Jia-Ning Xu Wei-Yu Yan Zi-Long Wang |
| author_facet | Zhi-Xian Gong Fu-Ping Cheng Jia-Ning Xu Wei-Yu Yan Zi-Long Wang |
| author_sort | Zhi-Xian Gong |
| collection | DOAJ |
| description | Honey bees are typical model organisms for the study of caste differentiation, and the juvenile hormone (JH) is a crucial link in the regulatory network of caste differentiation in honey bees. To investigate the mechanism of JH-mediated caste differentiation, we analyzed the effect of the JH response gene <i>AmKr-h1</i> on this process. We observed that <i>AmKr-h1</i> expression levels were significantly higher in queen larvae than in worker larvae at the 48 h, 84 h, and 120 h larval stages, and were regulated by JH. Inhibiting <i>AmKr-h1</i> expression in honey bee larvae using RNAi could lead to the development of larvae toward workers. We also analyzed the transcriptome changes in honey bee larvae after <i>AmKr-h1</i> RNAi and identified 191 differentially expressed genes (DEGs) and 682 differentially expressed alternative splicing events (DEASEs); of these, many were related to honey bee caste differentiation. Our results indicate that <i>AmKr-h1</i> regulates caste differentiation in honey bees by acting as a JH-responsive gene. |
| first_indexed | 2024-03-09T16:59:06Z |
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| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T16:59:06Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-ea63489319094a50b209d18d38d6acf42023-11-24T14:32:08ZengMDPI AGBiomolecules2218-273X2023-11-011311165710.3390/biom13111657The Juvenile-Hormone-Responsive Factor <i>AmKr-h1</i> Regulates Caste Differentiation in Honey BeesZhi-Xian Gong0Fu-Ping Cheng1Jia-Ning Xu2Wei-Yu Yan3Zi-Long Wang4Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, ChinaHoneybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, ChinaHoneybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, ChinaHoneybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, ChinaHoneybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, ChinaHoney bees are typical model organisms for the study of caste differentiation, and the juvenile hormone (JH) is a crucial link in the regulatory network of caste differentiation in honey bees. To investigate the mechanism of JH-mediated caste differentiation, we analyzed the effect of the JH response gene <i>AmKr-h1</i> on this process. We observed that <i>AmKr-h1</i> expression levels were significantly higher in queen larvae than in worker larvae at the 48 h, 84 h, and 120 h larval stages, and were regulated by JH. Inhibiting <i>AmKr-h1</i> expression in honey bee larvae using RNAi could lead to the development of larvae toward workers. We also analyzed the transcriptome changes in honey bee larvae after <i>AmKr-h1</i> RNAi and identified 191 differentially expressed genes (DEGs) and 682 differentially expressed alternative splicing events (DEASEs); of these, many were related to honey bee caste differentiation. Our results indicate that <i>AmKr-h1</i> regulates caste differentiation in honey bees by acting as a JH-responsive gene.https://www.mdpi.com/2218-273X/13/11/1657juvenile hormone<i>AmKr-h1</i>caste differentiationRNAiRNA-seq |
| spellingShingle | Zhi-Xian Gong Fu-Ping Cheng Jia-Ning Xu Wei-Yu Yan Zi-Long Wang The Juvenile-Hormone-Responsive Factor <i>AmKr-h1</i> Regulates Caste Differentiation in Honey Bees Biomolecules juvenile hormone <i>AmKr-h1</i> caste differentiation RNAi RNA-seq |
| title | The Juvenile-Hormone-Responsive Factor <i>AmKr-h1</i> Regulates Caste Differentiation in Honey Bees |
| title_full | The Juvenile-Hormone-Responsive Factor <i>AmKr-h1</i> Regulates Caste Differentiation in Honey Bees |
| title_fullStr | The Juvenile-Hormone-Responsive Factor <i>AmKr-h1</i> Regulates Caste Differentiation in Honey Bees |
| title_full_unstemmed | The Juvenile-Hormone-Responsive Factor <i>AmKr-h1</i> Regulates Caste Differentiation in Honey Bees |
| title_short | The Juvenile-Hormone-Responsive Factor <i>AmKr-h1</i> Regulates Caste Differentiation in Honey Bees |
| title_sort | juvenile hormone responsive factor i amkr h1 i regulates caste differentiation in honey bees |
| topic | juvenile hormone <i>AmKr-h1</i> caste differentiation RNAi RNA-seq |
| url | https://www.mdpi.com/2218-273X/13/11/1657 |
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