Acumulación de mercurio (Hg) por caña flecha (Gynerium sagittatum) (Aubl) Beauv. in vitro
<p class="MsoNormal" style="text-align: center; margin: 0cm 0cm 0pt;" align="center"><span style="font-size: small;"><span class="mediumtext"><strong style="mso-bidi-font-weight: normal;"><span style="font-fa...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | Spanish |
| Published: |
Universidad Nacional de Colombia
2011-04-01
|
| Series: | Revista Colombiana de Biotecnología |
| Subjects: | |
| Online Access: | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/22923 |
| _version_ | 1818528002205548544 |
|---|---|
| author | Rafael Esteban Ortega-Ortega Javier Darío Beltrán-Herrera José Luis Marrugo-Negrete |
| author_facet | Rafael Esteban Ortega-Ortega Javier Darío Beltrán-Herrera José Luis Marrugo-Negrete |
| author_sort | Rafael Esteban Ortega-Ortega |
| collection | DOAJ |
| description | <p class="MsoNormal" style="text-align: center; margin: 0cm 0cm 0pt;" align="center"><span style="font-size: small;"><span class="mediumtext"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif"; background: white; mso-ansi-language: EN-US;" lang="EN-US">Accumulation of mercury (Hg) by arrow cane (<em style="mso-bidi-font-style: normal;">Gynerium sagittatum</em>) (Aubl) Beauv. </span></strong></span><span class="mediumtext"><strong style="mso-bidi-font-weight: normal;"><em style="mso-bidi-font-style: normal;"><span style="font-family: "Times New Roman","serif"; mso-ansi-language: EN-US;" lang="EN-US">in vitro</span></em></strong></span><strong style="mso-bidi-font-weight: normal;"></strong></span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt;"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif"; mso-ansi-language: EN-US;" lang="EN-US"></span></strong></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt; mso-layout-grid-align: none;"> </p><p class="MsoNormal" style="text-align: center; margin: 0cm 0cm 0pt;" align="center"><strong style="mso-bidi-font-weight: normal;"></strong></p><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif";"><span style="font-size: small;">RESUMEN</span></span></strong><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif";"><span style="font-size: small;"> </span></span></strong><p> </p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><span style="font-size: small;"><span style="font-family: "Times New Roman","serif"; letter-spacing: -0.3pt;">En este estudio se evaluó, <em style="mso-bidi-font-style: normal;">in vitro</em>, la capacidad de acumulación de mercurio (Hg) que pueda poseer la caña flecha (<em style="mso-bidi-font-style: normal;">Gynerium sagittatum</em>) (Aubl) Beauv.<em style="mso-bidi-font-style: normal;"> </em>como una alternativa viable de ser implementada para la rehabilitación de suelos contaminados. Este estudio fue realizado en el laboratorio de Biotecnología Vegetal de la Universidad de Sucre (</span><span style="font-family: "Times New Roman","serif";">9º18’ N, 75º23’ O<span style="letter-spacing: -0.3pt;">). Se usó un diseño experimental de bloques al azar, </span>un análisis de varianza para comparación de medias, una prueba de Tukey (p≤0,05) para la establecer las diferencias significativas entre los tratamientos, <span style="color: #1f1a17;">pruebas de normalidad (Kolmogorov-Smirnov) y test de homogeneidad de varianza de Bartlett</span><span style="letter-spacing: -0.3pt;">.</span> Los análisis de mercurio se realizaron en un espectrofotómetro de absorción atómica Thermo Electron S4, provisto de una celda con ventanas de cuarzo, por el método de espectroscopia de absorción atómica con vapor frío. Los resultados muestran que la parte de la planta que presenta mayor acumulación es la raíz con </span><span style="font-family: "Times New Roman","serif"; mso-ansi-language: ES;" lang="ES">55,98 µg g<sup>-1</sup></span><span style="font-family: "Times New Roman","serif";"> HgT, seguida de los tallos-hojas con </span><span style="font-family: "Times New Roman","serif"; mso-ansi-language: ES;" lang="ES">14,84 µg g<sup>-1 </sup></span><span style="font-family: "Times New Roman","serif";">HgT, valores relacionados con el grado de concentración del metal en el medio de cultivo. Además, la acumulación de mercurio en raíces y tallos aumentó con el tiempo. En conclusión, <em style="mso-bidi-font-style: normal;">in vitro</em> esta planta acumula Hg debido a las altas concentraciones en sus tejidos sin afectar la viabilidad de las plantas.</span></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><span style="font-family: "Times New Roman","serif";"></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><span style="font-size: small;"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif";">Palabras clave</span></strong><span style="font-family: "Times New Roman","serif";">: fitorremediación, remoción, metales pesados.</span></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif";"></span></strong></p><p class="MsoNormal" style="margin: 0cm 0cm 10pt;"><span style="font-size: small;"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif"; mso-ansi-language: EN-US;" lang="EN-US">ABSTRACT</span></strong></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 10pt;"><span style="font-family: "Times New Roman","serif"; mso-ansi-language: EN;" lang="EN"><span style="font-size: small;">In this <em style="mso-bidi-font-style: normal;">in vitro</em> study was evaluated the potential for accumulation of mercury (Hg) that can hold the cane arrow (Gynerium sagittatum) (Aubl) Beauv. as a viable alternative to be implemented for the rehabilitation of contaminated soils. This study was conducted at the Plant Biotechnology Laboratory, University of Sucre (9º 18' N, 75° 23' W). An experimental design was randomized block, an analysis of variance to compare means, Tukey test (p ≤ 0.05) to establish significant differences between the treatments, tests of normality (Kolmogorov-Smirnov) and homogeneity test Bartlett variance. The mercury analyses were performed on atomic absorption spectrophotometer Thermo Electron S4, a cell equipped with quartz windows, by the method of atomic absorption spectrometry with cold steam. The results show that the plant part that presents the greatest accumulation is the root with 55.98 mg g-1 HgT, followed by the stems, leaves 14.84 g g-1 HgT and these values are related to the degree of metal concentration in the culture medium. Furthermore, the accumulation of mercury in roots and stems increased with time. In conclusion, <em style="mso-bidi-font-style: normal;">in vitro</em> this plant accumulates due to the high Hg concentrations in their tissues without affecting the viability of plants.</span></span></p><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif"; font-size: 12pt; mso-ansi-language: EN-US; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: ES; mso-bidi-language: AR-SA;" lang="EN-US">Key words</span></strong><span style="font-family: "Times New Roman","serif"; font-size: 12pt; mso-ansi-language: EN-US; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: ES; mso-bidi-language: AR-SA;" lang="EN-US">: Phytoremediation, removal, heavy metals.</span> |
| first_indexed | 2024-12-11T06:43:54Z |
| format | Article |
| id | doaj.art-ea65570e1dbd470fbf081e3223f60583 |
| institution | Directory Open Access Journal |
| issn | 0123-3475 1909-8758 |
| language | Spanish |
| last_indexed | 2024-12-11T06:43:54Z |
| publishDate | 2011-04-01 |
| publisher | Universidad Nacional de Colombia |
| record_format | Article |
| series | Revista Colombiana de Biotecnología |
| spelling | doaj.art-ea65570e1dbd470fbf081e3223f605832022-12-22T01:17:08ZspaUniversidad Nacional de ColombiaRevista Colombiana de Biotecnología0123-34751909-87582011-04-01131334120464Acumulación de mercurio (Hg) por caña flecha (Gynerium sagittatum) (Aubl) Beauv. in vitroRafael Esteban Ortega-Ortega0Javier Darío Beltrán-Herrera1José Luis Marrugo-Negrete2InvestigadorInvestigadorInvestigador<p class="MsoNormal" style="text-align: center; margin: 0cm 0cm 0pt;" align="center"><span style="font-size: small;"><span class="mediumtext"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif"; background: white; mso-ansi-language: EN-US;" lang="EN-US">Accumulation of mercury (Hg) by arrow cane (<em style="mso-bidi-font-style: normal;">Gynerium sagittatum</em>) (Aubl) Beauv. </span></strong></span><span class="mediumtext"><strong style="mso-bidi-font-weight: normal;"><em style="mso-bidi-font-style: normal;"><span style="font-family: "Times New Roman","serif"; mso-ansi-language: EN-US;" lang="EN-US">in vitro</span></em></strong></span><strong style="mso-bidi-font-weight: normal;"></strong></span></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt;"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif"; mso-ansi-language: EN-US;" lang="EN-US"></span></strong></p><p class="MsoNormal" style="margin: 0cm 0cm 0pt; mso-layout-grid-align: none;"> </p><p class="MsoNormal" style="text-align: center; margin: 0cm 0cm 0pt;" align="center"><strong style="mso-bidi-font-weight: normal;"></strong></p><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif";"><span style="font-size: small;">RESUMEN</span></span></strong><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif";"><span style="font-size: small;"> </span></span></strong><p> </p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><span style="font-size: small;"><span style="font-family: "Times New Roman","serif"; letter-spacing: -0.3pt;">En este estudio se evaluó, <em style="mso-bidi-font-style: normal;">in vitro</em>, la capacidad de acumulación de mercurio (Hg) que pueda poseer la caña flecha (<em style="mso-bidi-font-style: normal;">Gynerium sagittatum</em>) (Aubl) Beauv.<em style="mso-bidi-font-style: normal;"> </em>como una alternativa viable de ser implementada para la rehabilitación de suelos contaminados. Este estudio fue realizado en el laboratorio de Biotecnología Vegetal de la Universidad de Sucre (</span><span style="font-family: "Times New Roman","serif";">9º18’ N, 75º23’ O<span style="letter-spacing: -0.3pt;">). Se usó un diseño experimental de bloques al azar, </span>un análisis de varianza para comparación de medias, una prueba de Tukey (p≤0,05) para la establecer las diferencias significativas entre los tratamientos, <span style="color: #1f1a17;">pruebas de normalidad (Kolmogorov-Smirnov) y test de homogeneidad de varianza de Bartlett</span><span style="letter-spacing: -0.3pt;">.</span> Los análisis de mercurio se realizaron en un espectrofotómetro de absorción atómica Thermo Electron S4, provisto de una celda con ventanas de cuarzo, por el método de espectroscopia de absorción atómica con vapor frío. Los resultados muestran que la parte de la planta que presenta mayor acumulación es la raíz con </span><span style="font-family: "Times New Roman","serif"; mso-ansi-language: ES;" lang="ES">55,98 µg g<sup>-1</sup></span><span style="font-family: "Times New Roman","serif";"> HgT, seguida de los tallos-hojas con </span><span style="font-family: "Times New Roman","serif"; mso-ansi-language: ES;" lang="ES">14,84 µg g<sup>-1 </sup></span><span style="font-family: "Times New Roman","serif";">HgT, valores relacionados con el grado de concentración del metal en el medio de cultivo. Además, la acumulación de mercurio en raíces y tallos aumentó con el tiempo. En conclusión, <em style="mso-bidi-font-style: normal;">in vitro</em> esta planta acumula Hg debido a las altas concentraciones en sus tejidos sin afectar la viabilidad de las plantas.</span></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><span style="font-family: "Times New Roman","serif";"></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><span style="font-size: small;"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif";">Palabras clave</span></strong><span style="font-family: "Times New Roman","serif";">: fitorremediación, remoción, metales pesados.</span></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 0pt;"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif";"></span></strong></p><p class="MsoNormal" style="margin: 0cm 0cm 10pt;"><span style="font-size: small;"><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif"; mso-ansi-language: EN-US;" lang="EN-US">ABSTRACT</span></strong></span></p><p class="MsoNormal" style="text-align: justify; margin: 0cm 0cm 10pt;"><span style="font-family: "Times New Roman","serif"; mso-ansi-language: EN;" lang="EN"><span style="font-size: small;">In this <em style="mso-bidi-font-style: normal;">in vitro</em> study was evaluated the potential for accumulation of mercury (Hg) that can hold the cane arrow (Gynerium sagittatum) (Aubl) Beauv. as a viable alternative to be implemented for the rehabilitation of contaminated soils. This study was conducted at the Plant Biotechnology Laboratory, University of Sucre (9º 18' N, 75° 23' W). An experimental design was randomized block, an analysis of variance to compare means, Tukey test (p ≤ 0.05) to establish significant differences between the treatments, tests of normality (Kolmogorov-Smirnov) and homogeneity test Bartlett variance. The mercury analyses were performed on atomic absorption spectrophotometer Thermo Electron S4, a cell equipped with quartz windows, by the method of atomic absorption spectrometry with cold steam. The results show that the plant part that presents the greatest accumulation is the root with 55.98 mg g-1 HgT, followed by the stems, leaves 14.84 g g-1 HgT and these values are related to the degree of metal concentration in the culture medium. Furthermore, the accumulation of mercury in roots and stems increased with time. In conclusion, <em style="mso-bidi-font-style: normal;">in vitro</em> this plant accumulates due to the high Hg concentrations in their tissues without affecting the viability of plants.</span></span></p><strong style="mso-bidi-font-weight: normal;"><span style="font-family: "Times New Roman","serif"; font-size: 12pt; mso-ansi-language: EN-US; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: ES; mso-bidi-language: AR-SA;" lang="EN-US">Key words</span></strong><span style="font-family: "Times New Roman","serif"; font-size: 12pt; mso-ansi-language: EN-US; mso-fareast-font-family: 'Times New Roman'; mso-fareast-language: ES; mso-bidi-language: AR-SA;" lang="EN-US">: Phytoremediation, removal, heavy metals.</span>http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/22923Phytoremediationremovalheavy metalsfitorremediaciónremociónmetales pesados |
| spellingShingle | Rafael Esteban Ortega-Ortega Javier Darío Beltrán-Herrera José Luis Marrugo-Negrete Acumulación de mercurio (Hg) por caña flecha (Gynerium sagittatum) (Aubl) Beauv. in vitro Revista Colombiana de Biotecnología Phytoremediation removal heavy metals fitorremediación remoción metales pesados |
| title | Acumulación de mercurio (Hg) por caña flecha (Gynerium sagittatum) (Aubl) Beauv. in vitro |
| title_full | Acumulación de mercurio (Hg) por caña flecha (Gynerium sagittatum) (Aubl) Beauv. in vitro |
| title_fullStr | Acumulación de mercurio (Hg) por caña flecha (Gynerium sagittatum) (Aubl) Beauv. in vitro |
| title_full_unstemmed | Acumulación de mercurio (Hg) por caña flecha (Gynerium sagittatum) (Aubl) Beauv. in vitro |
| title_short | Acumulación de mercurio (Hg) por caña flecha (Gynerium sagittatum) (Aubl) Beauv. in vitro |
| title_sort | acumulacion de mercurio hg por cana flecha gynerium sagittatum aubl beauv in vitro |
| topic | Phytoremediation removal heavy metals fitorremediación remoción metales pesados |
| url | http://www.revistas.unal.edu.co/index.php/biotecnologia/article/view/22923 |
| work_keys_str_mv | AT rafaelestebanortegaortega acumulaciondemercuriohgporcanaflechagyneriumsagittatumaublbeauvinvitro AT javierdariobeltranherrera acumulaciondemercuriohgporcanaflechagyneriumsagittatumaublbeauvinvitro AT joseluismarrugonegrete acumulaciondemercuriohgporcanaflechagyneriumsagittatumaublbeauvinvitro |