Antioxidant-Activity-Guided Purification and Separation of Octocrylene from <i>Saussurea heteromalla</i>
Plants have been a source of medicine since ancient times, and such traditional medications are widely practiced nowadays. <i>Saussurea heteromalla</i> is widely used in traditional medicine in the Himalayan region for the treatment of different ailments. However, despite its traditional...
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MDPI AG
2023-02-01
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| Online Access: | https://www.mdpi.com/2297-8739/10/2/107 |
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| author | Saboon Yamin Bibi Samha Al Ayoubi Tayyiba Afzal Sobia Gilani Khafsa Malik Abdul Qayyum Mubashar Hussain Sunjeet Kumar |
| author_facet | Saboon Yamin Bibi Samha Al Ayoubi Tayyiba Afzal Sobia Gilani Khafsa Malik Abdul Qayyum Mubashar Hussain Sunjeet Kumar |
| author_sort | Saboon |
| collection | DOAJ |
| description | Plants have been a source of medicine since ancient times, and such traditional medications are widely practiced nowadays. <i>Saussurea heteromalla</i> is widely used in traditional medicine in the Himalayan region for the treatment of different ailments. However, despite its traditional uses, it is not widely explored for its free radical scavenging abilities and other biological activities. Thus, the current study is aimed at exploring the free radical scavenging ability of <i>S. heteromalla</i> extracts, along with the isolation and evaluation of its compound through bioassay-guided purification. From different solvent fractions, an n-hexane extract of a whole plant is found to be most active; thus, it is processed for large-scale extraction and column chromatography. Further, the purification of an active fraction is performed using HPLC–DAD, which led to the isolation of an active peak, identified by GC/MS, as 2-ethylhexyl 2-cyano-3, 3-diphenylprop-2-enoate. This compound, commonly known as octocrylene, is widely recommended for UV-B filter, to be used in the cosmetic industry as photoprotection products. The presence of free radical scavenging activity enhances the sun protection factor of octocrylene. The present study presents the first report on the isolation of this compound from the family Asteraceae. The compound has good free radical scavenging activity and is widely used in the cosmetic industry as a sun protectant and is much less reported from botanical sources. |
| first_indexed | 2024-03-11T08:10:19Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-03-11T08:10:19Z |
| publishDate | 2023-02-01 |
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| record_format | Article |
| series | Separations |
| spelling | doaj.art-ea66672ae28d4884aea9dbdde67bee802023-11-16T23:14:26ZengMDPI AGSeparations2297-87392023-02-0110210710.3390/separations10020107Antioxidant-Activity-Guided Purification and Separation of Octocrylene from <i>Saussurea heteromalla</i>Saboon0Yamin Bibi1Samha Al Ayoubi2Tayyiba Afzal3Sobia Gilani4Khafsa Malik5Abdul Qayyum6Mubashar Hussain7Sunjeet Kumar8Department of Botany, PMAS-Arid Agriculture University Rawalpindi, Rawalpindi 46300, PakistanDepartment of Botany, PMAS-Arid Agriculture University Rawalpindi, Rawalpindi 46300, PakistanCollege of Humanities and Sciences, Prince Sultan University, Riyadh 11586, Saudi ArabiaDepartment of Botany, PMAS-Arid Agriculture University Rawalpindi, Rawalpindi 46300, PakistanDepartment of Botany, PMAS-Arid Agriculture University Rawalpindi, Rawalpindi 46300, PakistanDepartment of Botany, PMAS-Arid Agriculture University Rawalpindi, Rawalpindi 46300, PakistanDepartment of Agronomy, The University of Haripur, Haripur 22620, PakistanDepartment of Zoology, University of Gujrat, Gujrat 50700, PakistanKey Laboratory for Quality Regulation of Tropical Horticultural Crops of Hainan Province, School of Horticulture, Hainan University, Haikou 570228, ChinaPlants have been a source of medicine since ancient times, and such traditional medications are widely practiced nowadays. <i>Saussurea heteromalla</i> is widely used in traditional medicine in the Himalayan region for the treatment of different ailments. However, despite its traditional uses, it is not widely explored for its free radical scavenging abilities and other biological activities. Thus, the current study is aimed at exploring the free radical scavenging ability of <i>S. heteromalla</i> extracts, along with the isolation and evaluation of its compound through bioassay-guided purification. From different solvent fractions, an n-hexane extract of a whole plant is found to be most active; thus, it is processed for large-scale extraction and column chromatography. Further, the purification of an active fraction is performed using HPLC–DAD, which led to the isolation of an active peak, identified by GC/MS, as 2-ethylhexyl 2-cyano-3, 3-diphenylprop-2-enoate. This compound, commonly known as octocrylene, is widely recommended for UV-B filter, to be used in the cosmetic industry as photoprotection products. The presence of free radical scavenging activity enhances the sun protection factor of octocrylene. The present study presents the first report on the isolation of this compound from the family Asteraceae. The compound has good free radical scavenging activity and is widely used in the cosmetic industry as a sun protectant and is much less reported from botanical sources.https://www.mdpi.com/2297-8739/10/2/107octocrylene<i>Saussurea heteromalla</i>sun protectantantioxidants |
| spellingShingle | Saboon Yamin Bibi Samha Al Ayoubi Tayyiba Afzal Sobia Gilani Khafsa Malik Abdul Qayyum Mubashar Hussain Sunjeet Kumar Antioxidant-Activity-Guided Purification and Separation of Octocrylene from <i>Saussurea heteromalla</i> Separations octocrylene <i>Saussurea heteromalla</i> sun protectant antioxidants |
| title | Antioxidant-Activity-Guided Purification and Separation of Octocrylene from <i>Saussurea heteromalla</i> |
| title_full | Antioxidant-Activity-Guided Purification and Separation of Octocrylene from <i>Saussurea heteromalla</i> |
| title_fullStr | Antioxidant-Activity-Guided Purification and Separation of Octocrylene from <i>Saussurea heteromalla</i> |
| title_full_unstemmed | Antioxidant-Activity-Guided Purification and Separation of Octocrylene from <i>Saussurea heteromalla</i> |
| title_short | Antioxidant-Activity-Guided Purification and Separation of Octocrylene from <i>Saussurea heteromalla</i> |
| title_sort | antioxidant activity guided purification and separation of octocrylene from i saussurea heteromalla i |
| topic | octocrylene <i>Saussurea heteromalla</i> sun protectant antioxidants |
| url | https://www.mdpi.com/2297-8739/10/2/107 |
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