Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomas
The complex interactions between cancer and host cells are far from being fully elucidated. Assessment of Th<sub>1</sub>/Th<sub>2</sub>/Th<sub>3</sub>/Tr<sub>1</sub> balance is an interest...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Via Medica
2011-08-01
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| Series: | Folia Histochemica et Cytobiologica |
| Subjects: | |
| Online Access: | http://czasopisma.viamedica.pl/fhc/article/view/4615 |
| _version_ | 1819154973463674880 |
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| author | Michał Matysiak Jerzy Kowalczyk Lech Chyczewski Iwona Malinowska Małgorzata Mitura-Lesiuk Maryna Krawczuk-Rybak Oksana Kovalchuk Włodzimierz Łuczyński |
| author_facet | Michał Matysiak Jerzy Kowalczyk Lech Chyczewski Iwona Malinowska Małgorzata Mitura-Lesiuk Maryna Krawczuk-Rybak Oksana Kovalchuk Włodzimierz Łuczyński |
| author_sort | Michał Matysiak |
| collection | DOAJ |
| description | The complex interactions between cancer and host cells are far from being fully elucidated. Assessment of Th<sub>1</sub>/Th<sub>2</sub>/Th<sub>3</sub>/Tr<sub>1</sub> balance is an interesting approach to explain immunological disturbances in lymphomas. The aim of our study was to assess mRNA for pro- and anti-inflammatory cytokines in T-cells in 20 children with Hodgkin- and non-Hodgkin lymphomas. CD4<sup>+</sup> and CD8<sup>+</sup> cells were isolated from whole peripheral blood and four different cytokine mRNA levels (IFN-&#947;, IL-10, IL-4, TGF-&#946;) were determined by real-time PCR technique. Comparing to the control group, we found lower expression of mRNA for IFN-gamma in CD4<sup>+</sup> cells at the time of lymphoma diagnosis. It may be one of the pathogenetic mechanisms of impaired immunity in these patients. |
| first_indexed | 2024-12-22T15:29:35Z |
| format | Article |
| id | doaj.art-ea674f7b281e445f962f2f34b3691ab2 |
| institution | Directory Open Access Journal |
| issn | 0239-8508 1897-5631 |
| language | English |
| last_indexed | 2024-12-22T15:29:35Z |
| publishDate | 2011-08-01 |
| publisher | Via Medica |
| record_format | Article |
| series | Folia Histochemica et Cytobiologica |
| spelling | doaj.art-ea674f7b281e445f962f2f34b3691ab22022-12-21T18:21:25ZengVia MedicaFolia Histochemica et Cytobiologica0239-85081897-56312011-08-0143316917110.5603/4615Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomasMichał MatysiakJerzy KowalczykLech ChyczewskiIwona MalinowskaMałgorzata Mitura-LesiukMaryna Krawczuk-RybakOksana KovalchukWłodzimierz ŁuczyńskiThe complex interactions between cancer and host cells are far from being fully elucidated. Assessment of Th<sub>1</sub>/Th<sub>2</sub>/Th<sub>3</sub>/Tr<sub>1</sub> balance is an interesting approach to explain immunological disturbances in lymphomas. The aim of our study was to assess mRNA for pro- and anti-inflammatory cytokines in T-cells in 20 children with Hodgkin- and non-Hodgkin lymphomas. CD4<sup>+</sup> and CD8<sup>+</sup> cells were isolated from whole peripheral blood and four different cytokine mRNA levels (IFN-&#947;, IL-10, IL-4, TGF-&#946;) were determined by real-time PCR technique. Comparing to the control group, we found lower expression of mRNA for IFN-gamma in CD4<sup>+</sup> cells at the time of lymphoma diagnosis. It may be one of the pathogenetic mechanisms of impaired immunity in these patients.http://czasopisma.viamedica.pl/fhc/article/view/4615LymphomaChildrenT-cellsInterferon-gammaImmunosuppression |
| spellingShingle | Michał Matysiak Jerzy Kowalczyk Lech Chyczewski Iwona Malinowska Małgorzata Mitura-Lesiuk Maryna Krawczuk-Rybak Oksana Kovalchuk Włodzimierz Łuczyński Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomas Folia Histochemica et Cytobiologica Lymphoma Children T-cells Interferon-gamma Immunosuppression |
| title | Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomas |
| title_full | Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomas |
| title_fullStr | Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomas |
| title_full_unstemmed | Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomas |
| title_short | Lower expression of mRNA for interferon-gamma in T helper cells of children with newly diagnosed lymphomas |
| title_sort | lower expression of mrna for interferon gamma in t helper cells of children with newly diagnosed lymphomas |
| topic | Lymphoma Children T-cells Interferon-gamma Immunosuppression |
| url | http://czasopisma.viamedica.pl/fhc/article/view/4615 |
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