Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma
Abstract Background Epstein–Barr virus (EBV) infection is causatively associated with a variety of human cancers, including gastric cancer (GC), which has one of the highest mortality rates of all human cancers. Long non-coding RNAs (lncRNAs) show important regulatory roles in human GC. SNHG8 is a r...
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| Format: | Article |
| Language: | English |
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BMC
2018-04-01
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| Series: | Cellular & Molecular Biology Letters |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s11658-018-0070-8 |
| _version_ | 1818614689884536832 |
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| author | Jing Liu Chunxia Yang Yufang Gu Chong Li Huamei Zhang Wenfang Zhang Xueqing Wang Nan Wu Chunyan Zheng |
| author_facet | Jing Liu Chunxia Yang Yufang Gu Chong Li Huamei Zhang Wenfang Zhang Xueqing Wang Nan Wu Chunyan Zheng |
| author_sort | Jing Liu |
| collection | DOAJ |
| description | Abstract Background Epstein–Barr virus (EBV) infection is causatively associated with a variety of human cancers, including gastric cancer (GC), which has one of the highest mortality rates of all human cancers. Long non-coding RNAs (lncRNAs) show important regulatory roles in human GC. SNHG8 is a recently identified lncRNA that was reported to show abnormal expression pattern in GC. However, little is known of its biological function in EBV-associated GC. Methods We used cell viability, colony formation and cell cycle assays to investigate the roles of lncRNA SNHG8 in the cell growth of EBV-associated GC. Results The transcript levels of SNHG8 in the cultured EBV-associated GC cells were significantly higher in the cultured EBV-associated GC cells compared with the levels in normal human gastric mucosal cells and EBV-negative GC cells. Knockdown of SNHG8 with specific shRNAs inhibited cell proliferation and colony formation and arrested the cell cycle in the G0/G1 phase in vitro. We also found that knockdown of SNHG8 suppressed tumor growth in vivo. Conclusions These data indicate the pro-oncogenic potential of SNHG8 in EBV-associated GC, meaning it is a latent therapeutic target for the treatment of this type of cancer. |
| first_indexed | 2024-12-16T16:22:01Z |
| format | Article |
| id | doaj.art-ea6841178d244384837b67bc28dd62a4 |
| institution | Directory Open Access Journal |
| issn | 1425-8153 1689-1392 |
| language | English |
| last_indexed | 2024-12-16T16:22:01Z |
| publishDate | 2018-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Cellular & Molecular Biology Letters |
| spelling | doaj.art-ea6841178d244384837b67bc28dd62a42022-12-21T22:24:53ZengBMCCellular & Molecular Biology Letters1425-81531689-13922018-04-0123111010.1186/s11658-018-0070-8Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinomaJing Liu0Chunxia Yang1Yufang Gu2Chong Li3Huamei Zhang4Wenfang Zhang5Xueqing Wang6Nan Wu7Chunyan Zheng8Department of NephologyDepartment of NephologyDepartment of Gastrointestinal SurgeryDepartment of NephologyDepartment of NephologyDepartment of NephologyDepartment of NephologyDepartment of NephologyDepartment of Medical Care, Zibo Central HospitalAbstract Background Epstein–Barr virus (EBV) infection is causatively associated with a variety of human cancers, including gastric cancer (GC), which has one of the highest mortality rates of all human cancers. Long non-coding RNAs (lncRNAs) show important regulatory roles in human GC. SNHG8 is a recently identified lncRNA that was reported to show abnormal expression pattern in GC. However, little is known of its biological function in EBV-associated GC. Methods We used cell viability, colony formation and cell cycle assays to investigate the roles of lncRNA SNHG8 in the cell growth of EBV-associated GC. Results The transcript levels of SNHG8 in the cultured EBV-associated GC cells were significantly higher in the cultured EBV-associated GC cells compared with the levels in normal human gastric mucosal cells and EBV-negative GC cells. Knockdown of SNHG8 with specific shRNAs inhibited cell proliferation and colony formation and arrested the cell cycle in the G0/G1 phase in vitro. We also found that knockdown of SNHG8 suppressed tumor growth in vivo. Conclusions These data indicate the pro-oncogenic potential of SNHG8 in EBV-associated GC, meaning it is a latent therapeutic target for the treatment of this type of cancer.http://link.springer.com/article/10.1186/s11658-018-0070-8SNHG8Cell growthshRNAEpstein-Barr virus-associated gastric carcinoma |
| spellingShingle | Jing Liu Chunxia Yang Yufang Gu Chong Li Huamei Zhang Wenfang Zhang Xueqing Wang Nan Wu Chunyan Zheng Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma Cellular & Molecular Biology Letters SNHG8 Cell growth shRNA Epstein-Barr virus-associated gastric carcinoma |
| title | Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma |
| title_full | Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma |
| title_fullStr | Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma |
| title_full_unstemmed | Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma |
| title_short | Knockdown of the lncRNA SNHG8 inhibits cell growth in Epstein-Barr virus-associated gastric carcinoma |
| title_sort | knockdown of the lncrna snhg8 inhibits cell growth in epstein barr virus associated gastric carcinoma |
| topic | SNHG8 Cell growth shRNA Epstein-Barr virus-associated gastric carcinoma |
| url | http://link.springer.com/article/10.1186/s11658-018-0070-8 |
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