Inhibitory Effects on Oral Microbial Activity and Production of Lipopolysaccharides-Induced Pro-Inflammatory Mediators in Raw264.7 Macrophages of Ethanol Extract of Perilla flutescens (L.) Britton
Background: The leaves of Perilla frutescens, commonly called perilla and used for food in Korea, contain components with a variety of biological effects and potential therapeutic applications. The purpose of this study was to identify the components of 70% ethanol extracted Perilla frutescens (EEPF...
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The Korean Society of Dental Hygiene Science
2020-12-01
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| Series: | 치위생과학회지 |
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| _version_ | 1818388448480854016 |
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| author | Moon-Jin Jeong Do-Seon Lim Myoung-Hwa Lee Kyungwon Heo Han-Hong Kim Soon-Jeong Jeong |
| author_facet | Moon-Jin Jeong Do-Seon Lim Myoung-Hwa Lee Kyungwon Heo Han-Hong Kim Soon-Jeong Jeong |
| author_sort | Moon-Jin Jeong |
| collection | DOAJ |
| description | Background: The leaves of Perilla frutescens, commonly called perilla and used for food in Korea, contain components with a variety of biological effects and potential therapeutic applications. The purpose of this study was to identify the components of 70% ethanol extracted Perilla frutescens (EEPF) and determine its inhibitory effects on oral microbial activity and production of nitric oxide (NO) and prostaglandin E2 (PGE2) in lipopolysaccharides (LPS)-stimulated Raw264.7 macrophages, consequently, to confirm the possibility of using EEPF as a functional component for improving the oral environment and preventing inflammation.Methods: One kg of P. frutescens leaves was extracted with 70% ethanol and dried at −70oC. EEPF was analyzed using high-performance liquid chromatography analysis, and antimicrobial activity against oral microorganisms was revealed using the disk diffusion test. Cell viability was elucidated using a methylthiazolydiphenyl-tetrazolium bromide assay, and the effect of EEPF on LPS-induced morphological variation was confirmed through microscopic observation. The effect of EEPF on LPS-induced production of pro-inflammatory mediators, NO and PGE2 was confirmed by the NO assay and PGE2 enzyme-linked immunosorbent assay.Results: The main component of EEPF was rosemarinic acid, and EEPF showed weak anti-bacterial and anti-fungal effects against microorganisms living in the oral cavity. EEPF did not show toxicity to Raw264.7 macrophages and had inhibitory effects on the morphological variations and production of pro-inflammatory mediators, NO and PGE2 in LPS-stimulated Raw264.7 macrophages.Conclusion: EEPF can be used as a functional material for improving the oral environment through the control of oral microorganisms and for modulating inflammation by inhibiting the production of inflammatory mediators. |
| first_indexed | 2024-12-14T04:26:00Z |
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| id | doaj.art-ea6a52e4bde5443994c4b4204bdae9dc |
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| issn | 2233-7679 |
| language | English |
| last_indexed | 2024-12-14T04:26:00Z |
| publishDate | 2020-12-01 |
| publisher | The Korean Society of Dental Hygiene Science |
| record_format | Article |
| series | 치위생과학회지 |
| spelling | doaj.art-ea6a52e4bde5443994c4b4204bdae9dc2022-12-21T23:17:12ZengThe Korean Society of Dental Hygiene Science치위생과학회지2233-76792020-12-0120421322010.17135/jdhs.2020.20.4.213Inhibitory Effects on Oral Microbial Activity and Production of Lipopolysaccharides-Induced Pro-Inflammatory Mediators in Raw264.7 Macrophages of Ethanol Extract of Perilla flutescens (L.) BrittonMoon-Jin Jeong0https://orcid.org/0000-0002-5547-898XDo-Seon Lim1https://orcid.org/0000-0003-4602-3323Myoung-Hwa Lee2https://orcid.org/0000-0003-2291-5589Kyungwon Heo3https://orcid.org/0000-0003-0214-2216Han-Hong Kim4https://orcid.org/0000-0003-2193-8092Soon-Jeong Jeong5https://orcid.org/0000-0002-8959-4663Department of Oral Histology and Developmental Biology, School of Dentistry, Chosun University, Gwangju 61452, KoreaDepartment of Dental Hygiene, Graduate School of Public Health Science, Eulji University, Seongnam 13135, KoreaDepartment of Oral Histology and Developmental Biology, School of Dentistry, Chosun University, Gwangju 61452, KoreaDepartment of Oral Histology and Developmental Biology, School of Dentistry, Chosun University, Gwangju 61452, KoreaDepartment of Dental Hygiene, Hyejeon College, Hongseong 32244, KoreaDepartment of Dental Hygiene & Institute of Basic Science for Well-Aging, Youngsan University, Yangsan 50510, KoreaBackground: The leaves of Perilla frutescens, commonly called perilla and used for food in Korea, contain components with a variety of biological effects and potential therapeutic applications. The purpose of this study was to identify the components of 70% ethanol extracted Perilla frutescens (EEPF) and determine its inhibitory effects on oral microbial activity and production of nitric oxide (NO) and prostaglandin E2 (PGE2) in lipopolysaccharides (LPS)-stimulated Raw264.7 macrophages, consequently, to confirm the possibility of using EEPF as a functional component for improving the oral environment and preventing inflammation.Methods: One kg of P. frutescens leaves was extracted with 70% ethanol and dried at −70oC. EEPF was analyzed using high-performance liquid chromatography analysis, and antimicrobial activity against oral microorganisms was revealed using the disk diffusion test. Cell viability was elucidated using a methylthiazolydiphenyl-tetrazolium bromide assay, and the effect of EEPF on LPS-induced morphological variation was confirmed through microscopic observation. The effect of EEPF on LPS-induced production of pro-inflammatory mediators, NO and PGE2 was confirmed by the NO assay and PGE2 enzyme-linked immunosorbent assay.Results: The main component of EEPF was rosemarinic acid, and EEPF showed weak anti-bacterial and anti-fungal effects against microorganisms living in the oral cavity. EEPF did not show toxicity to Raw264.7 macrophages and had inhibitory effects on the morphological variations and production of pro-inflammatory mediators, NO and PGE2 in LPS-stimulated Raw264.7 macrophages.Conclusion: EEPF can be used as a functional material for improving the oral environment through the control of oral microorganisms and for modulating inflammation by inhibiting the production of inflammatory mediators.oral microbial activityperilla frutescenspro-inflammatory mediatorraw264.7 cellsrosmarinic acid |
| spellingShingle | Moon-Jin Jeong Do-Seon Lim Myoung-Hwa Lee Kyungwon Heo Han-Hong Kim Soon-Jeong Jeong Inhibitory Effects on Oral Microbial Activity and Production of Lipopolysaccharides-Induced Pro-Inflammatory Mediators in Raw264.7 Macrophages of Ethanol Extract of Perilla flutescens (L.) Britton 치위생과학회지 oral microbial activity perilla frutescens pro-inflammatory mediator raw264.7 cells rosmarinic acid |
| title | Inhibitory Effects on Oral Microbial Activity and Production of Lipopolysaccharides-Induced Pro-Inflammatory Mediators in Raw264.7 Macrophages of Ethanol Extract of Perilla flutescens (L.) Britton |
| title_full | Inhibitory Effects on Oral Microbial Activity and Production of Lipopolysaccharides-Induced Pro-Inflammatory Mediators in Raw264.7 Macrophages of Ethanol Extract of Perilla flutescens (L.) Britton |
| title_fullStr | Inhibitory Effects on Oral Microbial Activity and Production of Lipopolysaccharides-Induced Pro-Inflammatory Mediators in Raw264.7 Macrophages of Ethanol Extract of Perilla flutescens (L.) Britton |
| title_full_unstemmed | Inhibitory Effects on Oral Microbial Activity and Production of Lipopolysaccharides-Induced Pro-Inflammatory Mediators in Raw264.7 Macrophages of Ethanol Extract of Perilla flutescens (L.) Britton |
| title_short | Inhibitory Effects on Oral Microbial Activity and Production of Lipopolysaccharides-Induced Pro-Inflammatory Mediators in Raw264.7 Macrophages of Ethanol Extract of Perilla flutescens (L.) Britton |
| title_sort | inhibitory effects on oral microbial activity and production of lipopolysaccharides induced pro inflammatory mediators in raw264 7 macrophages of ethanol extract of perilla flutescens l britton |
| topic | oral microbial activity perilla frutescens pro-inflammatory mediator raw264.7 cells rosmarinic acid |
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