Antibacterial Activity of Indolicidin-Coated Silver Nanoparticles in Oral Disease
(1) Background: In dentistry, silver nanoparticles (AgNPs) have progressively earned great interest as antimicrobial drugs and are widely used in several biomedical fields. Recent progress in the analysis of complex bacterial communities has demonstrated the richness of the oral microbiota and the p...
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| Format: | Article |
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MDPI AG
2020-03-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/10/5/1837 |
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| author | Carla Zannella Surbhi Shinde Mariateresa Vitiello Annarita Falanga Emilia Galdiero Amir Fahmi Biagio Santella Ludovica Nucci Roberta Gasparro Marilena Galdiero Mariarosaria Boccellino Gianluigi Franci Marina Di Domenico |
| author_facet | Carla Zannella Surbhi Shinde Mariateresa Vitiello Annarita Falanga Emilia Galdiero Amir Fahmi Biagio Santella Ludovica Nucci Roberta Gasparro Marilena Galdiero Mariarosaria Boccellino Gianluigi Franci Marina Di Domenico |
| author_sort | Carla Zannella |
| collection | DOAJ |
| description | (1) Background: In dentistry, silver nanoparticles (AgNPs) have progressively earned great interest as antimicrobial drugs and are widely used in several biomedical fields. Recent progress in the analysis of complex bacterial communities has demonstrated the richness of the oral microbiota and the presence of numerous previously unexplained strains. Several efforts have been dedicated to the investigation of antimicrobial peptides (AMPs). Those peptides are a widespread group of small peptides against invading microbes. We report the production of a hybrid molecule composed of AgNPs and indolicidin, a well-known antibacterial peptide. (2) Methods: Spectroscopy and microscopy were used to analyze the optical features and to determine the size of the generated AgNPs. The AgNP antibacterial activity was evaluated versus oral Gram-positive and Gram-negative bacteria. (3) Results: The coated nanoparticles’ antibacterial activity strongly inhibited the growth of microorganisms, with very low minimum inhibitory concentration (MIC) values in the range of 5−12.5 μg/mL. We hypothesize that this effect depended on the specific characteristics of the metal surface coated with indolicidin. The second result was that the coated nanoparticles observed cellular toxicity, was lower with respect to the toxicity of peptide and the naked AgNPs when used individually. (4) New investigations regarding antimicrobial effect of AgNPs coated with AMPs in oral infections are an urgent task. |
| first_indexed | 2024-12-12T23:50:58Z |
| format | Article |
| id | doaj.art-ea6b874545684c669bd447f0f7c83205 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-12T23:50:58Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-ea6b874545684c669bd447f0f7c832052022-12-22T00:06:41ZengMDPI AGApplied Sciences2076-34172020-03-01105183710.3390/app10051837app10051837Antibacterial Activity of Indolicidin-Coated Silver Nanoparticles in Oral DiseaseCarla Zannella0Surbhi Shinde1Mariateresa Vitiello2Annarita Falanga3Emilia Galdiero4Amir Fahmi5Biagio Santella6Ludovica Nucci7Roberta Gasparro8Marilena Galdiero9Mariarosaria Boccellino10Gianluigi Franci11Marina Di Domenico12Department of Experimental Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, ItalyDepartment of Experimental Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, ItalyDepartment of Clinical Pathology, Virology Unit, San Giovanni di Dio e Ruggi d’Aragona Hospital, 84131 Salerno, ItalyDepartment of Agricultural Sciences, University of Naples Federico II, 80055 Portici, ItalyDepartment of Biology, University of Naples Federico II, 80126 Naples, ItalyFaculty of Technology and Bionics, Rhine-Waal University of Applied Science, 47533 Kleve, GermanyDepartment of Experimental Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, ItalyMultidisciplinary Department of Medico-Surgical and Dental Specialties, University of Campania Luigi Vanvitelli, 80138 Naples, ItalyDepartment of Neuroscience, Reproductive Science and Odontostomatology, University of Naples Federico II, 80131 Naples, ItalyDepartment of Experimental Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, ItalyDepartment of Precision Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, ItalyDepartment of Medicine, Surgery and Dentistry “Scuola Medica Salernitana”/DIPMED, University of Salerno, 84081 Baronissi, ItalyDepartment of Precision Medicine, University of Campania Luigi Vanvitelli, 80138 Naples, Italy(1) Background: In dentistry, silver nanoparticles (AgNPs) have progressively earned great interest as antimicrobial drugs and are widely used in several biomedical fields. Recent progress in the analysis of complex bacterial communities has demonstrated the richness of the oral microbiota and the presence of numerous previously unexplained strains. Several efforts have been dedicated to the investigation of antimicrobial peptides (AMPs). Those peptides are a widespread group of small peptides against invading microbes. We report the production of a hybrid molecule composed of AgNPs and indolicidin, a well-known antibacterial peptide. (2) Methods: Spectroscopy and microscopy were used to analyze the optical features and to determine the size of the generated AgNPs. The AgNP antibacterial activity was evaluated versus oral Gram-positive and Gram-negative bacteria. (3) Results: The coated nanoparticles’ antibacterial activity strongly inhibited the growth of microorganisms, with very low minimum inhibitory concentration (MIC) values in the range of 5−12.5 μg/mL. We hypothesize that this effect depended on the specific characteristics of the metal surface coated with indolicidin. The second result was that the coated nanoparticles observed cellular toxicity, was lower with respect to the toxicity of peptide and the naked AgNPs when used individually. (4) New investigations regarding antimicrobial effect of AgNPs coated with AMPs in oral infections are an urgent task.https://www.mdpi.com/2076-3417/10/5/1837antimicrobial peptidesilver nanoparticlesgram-negative bacteriagram-positive bacteriaoral disease |
| spellingShingle | Carla Zannella Surbhi Shinde Mariateresa Vitiello Annarita Falanga Emilia Galdiero Amir Fahmi Biagio Santella Ludovica Nucci Roberta Gasparro Marilena Galdiero Mariarosaria Boccellino Gianluigi Franci Marina Di Domenico Antibacterial Activity of Indolicidin-Coated Silver Nanoparticles in Oral Disease Applied Sciences antimicrobial peptide silver nanoparticles gram-negative bacteria gram-positive bacteria oral disease |
| title | Antibacterial Activity of Indolicidin-Coated Silver Nanoparticles in Oral Disease |
| title_full | Antibacterial Activity of Indolicidin-Coated Silver Nanoparticles in Oral Disease |
| title_fullStr | Antibacterial Activity of Indolicidin-Coated Silver Nanoparticles in Oral Disease |
| title_full_unstemmed | Antibacterial Activity of Indolicidin-Coated Silver Nanoparticles in Oral Disease |
| title_short | Antibacterial Activity of Indolicidin-Coated Silver Nanoparticles in Oral Disease |
| title_sort | antibacterial activity of indolicidin coated silver nanoparticles in oral disease |
| topic | antimicrobial peptide silver nanoparticles gram-negative bacteria gram-positive bacteria oral disease |
| url | https://www.mdpi.com/2076-3417/10/5/1837 |
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