miR-491-5p regulates the sensitivity of human bladder cancer cell line EJ to 5-fluorouracil
Objective To investigate the role and mechanism of miR-491-5p on 5-fluorouracil (5-FU) sensitivity in human bladder cancer cell line EJ. Methods The expressions of miR-491-5p in chemotherapy-sensitive and resistant tissues were detected by RT-qPCR. The 5-FU resistant EJ/5-FU cell line was construc...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2021-02-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200253.pdf |
| _version_ | 1827390622499078144 |
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| author | YANG Feng, XU Jia, QIU Guo-hui, YANG Zhi-ling |
| author_facet | YANG Feng, XU Jia, QIU Guo-hui, YANG Zhi-ling |
| author_sort | YANG Feng, XU Jia, QIU Guo-hui, YANG Zhi-ling |
| collection | DOAJ |
| description | Objective To investigate the role and mechanism of miR-491-5p on 5-fluorouracil (5-FU) sensitivity in human bladder cancer cell line EJ. Methods The expressions of miR-491-5p in chemotherapy-sensitive and resistant tissues were detected by RT-qPCR. The 5-FU resistant EJ/5-FU cell line was constructed, the miR-491-5p expression and phosphorylation of AKT and STAT3 were detected by RT-qPCR and Western blot, respectively. After miR-491-5p inhibitor was transfected into EJ cells or miR-491-5p mimic was transfected into EJ/5-FU cells, the cell viability, apoptosis, wound healing, migration, invasion, AKT and STAT3 phosphorylation levels were detected by CCK-8 assay, Annexin V-FITC/PI staining, scratch assay and Transwell assay and Western blot under the condition of 5-FU, respectively. Results The expression of miR-491-5p was significantly decreased in chemotherapy-resistant tissues and EJ/5-FU cell line(P＜0.01). After down-regulation of miR-491-5p in EJ cells, the inhibition of 5-FU on cell viability, wound healing, migration and invasion, and the promotion of apoptosis were significantly reduced (P＜0.01). After up-regulation of miR-491-5p in EJ/5-FU cell line, the inhibition of 5-FU on cell viability, wound healing, migration and invasion, and the promotion of apoptosis were significantly increased(P＜0.01). The phosphorylation of AKT and STAT3 was significantly increased in EJ/5-FU cell line(P＜0.01); the phosphorylation of AKT and STAT3 in cell line EJ was significantly increased after the down-regulation of miR-491-5p (P＜0.01), and the phosphorylation of AKT and STAT3 in EJ/5-FU cell line was significantly decreased after the up-regulation of miR-491-5p (P＜0.01). Conclusions Down-regulation of miR-491-5p reduces the sensitivity of cell line EJ to 5-FU, while up-regulation of miR-491-5p inhibits 5-FU resistance in EJ/5-FU cell line. miR-491-5p in regulation of 5-FU resistance of cell line EJ is accompanied by changes of AKT and STAT3 phosphorylation. |
| first_indexed | 2024-03-08T16:55:26Z |
| format | Article |
| id | doaj.art-ea6beb8d51b542f783a4271e1e351d2b |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:55:26Z |
| publishDate | 2021-02-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-ea6beb8d51b542f783a4271e1e351d2b2024-01-05T03:04:29ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252021-02-01412233239miR-491-5p regulates the sensitivity of human bladder cancer cell line EJ to 5-fluorouracilYANG Feng, XU Jia, QIU Guo-hui, YANG Zhi-ling0No. 2 Department of Pharmacy, Hunan People's Hospital, Changsha 410002, ChinaObjective To investigate the role and mechanism of miR-491-5p on 5-fluorouracil (5-FU) sensitivity in human bladder cancer cell line EJ. Methods The expressions of miR-491-5p in chemotherapy-sensitive and resistant tissues were detected by RT-qPCR. The 5-FU resistant EJ/5-FU cell line was constructed, the miR-491-5p expression and phosphorylation of AKT and STAT3 were detected by RT-qPCR and Western blot, respectively. After miR-491-5p inhibitor was transfected into EJ cells or miR-491-5p mimic was transfected into EJ/5-FU cells, the cell viability, apoptosis, wound healing, migration, invasion, AKT and STAT3 phosphorylation levels were detected by CCK-8 assay, Annexin V-FITC/PI staining, scratch assay and Transwell assay and Western blot under the condition of 5-FU, respectively. Results The expression of miR-491-5p was significantly decreased in chemotherapy-resistant tissues and EJ/5-FU cell line(P＜0.01). After down-regulation of miR-491-5p in EJ cells, the inhibition of 5-FU on cell viability, wound healing, migration and invasion, and the promotion of apoptosis were significantly reduced (P＜0.01). After up-regulation of miR-491-5p in EJ/5-FU cell line, the inhibition of 5-FU on cell viability, wound healing, migration and invasion, and the promotion of apoptosis were significantly increased(P＜0.01). The phosphorylation of AKT and STAT3 was significantly increased in EJ/5-FU cell line(P＜0.01); the phosphorylation of AKT and STAT3 in cell line EJ was significantly increased after the down-regulation of miR-491-5p (P＜0.01), and the phosphorylation of AKT and STAT3 in EJ/5-FU cell line was significantly decreased after the up-regulation of miR-491-5p (P＜0.01). Conclusions Down-regulation of miR-491-5p reduces the sensitivity of cell line EJ to 5-FU, while up-regulation of miR-491-5p inhibits 5-FU resistance in EJ/5-FU cell line. miR-491-5p in regulation of 5-FU resistance of cell line EJ is accompanied by changes of AKT and STAT3 phosphorylation.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200253.pdfmir-491-5p|bladder cancer|5-fluorouracil |
| spellingShingle | YANG Feng, XU Jia, QIU Guo-hui, YANG Zhi-ling miR-491-5p regulates the sensitivity of human bladder cancer cell line EJ to 5-fluorouracil Jichu yixue yu linchuang mir-491-5p|bladder cancer|5-fluorouracil |
| title | miR-491-5p regulates the sensitivity of human bladder cancer cell line EJ to 5-fluorouracil |
| title_full | miR-491-5p regulates the sensitivity of human bladder cancer cell line EJ to 5-fluorouracil |
| title_fullStr | miR-491-5p regulates the sensitivity of human bladder cancer cell line EJ to 5-fluorouracil |
| title_full_unstemmed | miR-491-5p regulates the sensitivity of human bladder cancer cell line EJ to 5-fluorouracil |
| title_short | miR-491-5p regulates the sensitivity of human bladder cancer cell line EJ to 5-fluorouracil |
| title_sort | mir 491 5p regulates the sensitivity of human bladder cancer cell line ej to 5 fluorouracil |
| topic | mir-491-5p|bladder cancer|5-fluorouracil |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/a200253.pdf |
| work_keys_str_mv | AT yangfengxujiaqiuguohuiyangzhiling mir4915pregulatesthesensitivityofhumanbladdercancercelllineejto5fluorouracil |