Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations
The nucleopore is an essential structure of the eukaryotic cell, regulating passage between the nucleus and cytoplasm. While individual functions of core nucleopore proteins have been identified, the role of other components, such as Nup210, are poorly defined. Here, through the use of an unbiased E...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2018-09-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2018.02234/full |
| _version_ | 1818582685555097600 |
|---|---|
| author | Annemarie van Nieuwenhuijze Annemarie van Nieuwenhuijze Oliver Burton Oliver Burton Pierre Lemaitre Pierre Lemaitre Alice E. Denton Ana Cascalho Ana Cascalho Rose E. Goodchild Rose E. Goodchild Bert Malengier-Devlies Bert Malengier-Devlies Bénédicte Cauwe Bénédicte Cauwe Michelle A. Linterman Stephanie Humblet-Baron Stephanie Humblet-Baron Adrian Liston Adrian Liston |
| author_facet | Annemarie van Nieuwenhuijze Annemarie van Nieuwenhuijze Oliver Burton Oliver Burton Pierre Lemaitre Pierre Lemaitre Alice E. Denton Ana Cascalho Ana Cascalho Rose E. Goodchild Rose E. Goodchild Bert Malengier-Devlies Bert Malengier-Devlies Bénédicte Cauwe Bénédicte Cauwe Michelle A. Linterman Stephanie Humblet-Baron Stephanie Humblet-Baron Adrian Liston Adrian Liston |
| author_sort | Annemarie van Nieuwenhuijze |
| collection | DOAJ |
| description | The nucleopore is an essential structure of the eukaryotic cell, regulating passage between the nucleus and cytoplasm. While individual functions of core nucleopore proteins have been identified, the role of other components, such as Nup210, are poorly defined. Here, through the use of an unbiased ENU mutagenesis screen for mutations effecting the peripheral T cell compartment, we identified a Nup210 mutation in a mouse strain with altered CD4/CD8 T cell ratios. Through the generation of Nup210 knockout mice we identified Nup210 as having a T cell-intrinsic function in the peripheral homeostasis of T cells. Remarkably, despite the deep evolutionary conservation of this key nucleopore complex member, no other major phenotypes developed, with viable and healthy knockout mice. These results identify Nup210 as an important nucleopore complex component for peripheral T cells, and raise further questions of why this nucleopore component shows deep evolutionary conservation despite seemingly redundant functions in most cell types. |
| first_indexed | 2024-12-16T07:53:19Z |
| format | Article |
| id | doaj.art-ea6daabe68c0476eac4279b06148b9be |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-16T07:53:19Z |
| publishDate | 2018-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-ea6daabe68c0476eac4279b06148b9be2022-12-21T22:38:48ZengFrontiers Media S.A.Frontiers in Immunology1664-32242018-09-01910.3389/fimmu.2018.02234380797Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell AlterationsAnnemarie van Nieuwenhuijze0Annemarie van Nieuwenhuijze1Oliver Burton2Oliver Burton3Pierre Lemaitre4Pierre Lemaitre5Alice E. Denton6Ana Cascalho7Ana Cascalho8Rose E. Goodchild9Rose E. Goodchild10Bert Malengier-Devlies11Bert Malengier-Devlies12Bénédicte Cauwe13Bénédicte Cauwe14Michelle A. Linterman15Stephanie Humblet-Baron16Stephanie Humblet-Baron17Adrian Liston18Adrian Liston19VIB Centre for Brain & Disease Research, VIB, Leuven, BelgiumDepartment of Microbiology and ImmunologyUniversity of Leuven, Leuven, BelgiumVIB Centre for Brain & Disease Research, VIB, Leuven, BelgiumDepartment of Microbiology and ImmunologyUniversity of Leuven, Leuven, BelgiumVIB Centre for Brain & Disease Research, VIB, Leuven, BelgiumDepartment of Microbiology and ImmunologyUniversity of Leuven, Leuven, BelgiumBabraham Institute, Babraham Research Campus, Cambridge, United KingdomVIB Centre for Brain & Disease Research, VIB, Leuven, BelgiumDepartment of Neurosciences, University of Leuven, Leuven, BelgiumVIB Centre for Brain & Disease Research, VIB, Leuven, BelgiumDepartment of Neurosciences, University of Leuven, Leuven, BelgiumVIB Centre for Brain & Disease Research, VIB, Leuven, BelgiumDepartment of Microbiology and ImmunologyUniversity of Leuven, Leuven, BelgiumVIB Centre for Brain & Disease Research, VIB, Leuven, BelgiumDepartment of Microbiology and ImmunologyUniversity of Leuven, Leuven, BelgiumBabraham Institute, Babraham Research Campus, Cambridge, United KingdomVIB Centre for Brain & Disease Research, VIB, Leuven, BelgiumDepartment of Microbiology and ImmunologyUniversity of Leuven, Leuven, BelgiumVIB Centre for Brain & Disease Research, VIB, Leuven, BelgiumDepartment of Microbiology and ImmunologyUniversity of Leuven, Leuven, BelgiumThe nucleopore is an essential structure of the eukaryotic cell, regulating passage between the nucleus and cytoplasm. While individual functions of core nucleopore proteins have been identified, the role of other components, such as Nup210, are poorly defined. Here, through the use of an unbiased ENU mutagenesis screen for mutations effecting the peripheral T cell compartment, we identified a Nup210 mutation in a mouse strain with altered CD4/CD8 T cell ratios. Through the generation of Nup210 knockout mice we identified Nup210 as having a T cell-intrinsic function in the peripheral homeostasis of T cells. Remarkably, despite the deep evolutionary conservation of this key nucleopore complex member, no other major phenotypes developed, with viable and healthy knockout mice. These results identify Nup210 as an important nucleopore complex component for peripheral T cells, and raise further questions of why this nucleopore component shows deep evolutionary conservation despite seemingly redundant functions in most cell types.https://www.frontiersin.org/article/10.3389/fimmu.2018.02234/fullNup210CD4-positive T-lymphocytesCD8-positive T-lymphocytesthymus glandspleennucleopore |
| spellingShingle | Annemarie van Nieuwenhuijze Annemarie van Nieuwenhuijze Oliver Burton Oliver Burton Pierre Lemaitre Pierre Lemaitre Alice E. Denton Ana Cascalho Ana Cascalho Rose E. Goodchild Rose E. Goodchild Bert Malengier-Devlies Bert Malengier-Devlies Bénédicte Cauwe Bénédicte Cauwe Michelle A. Linterman Stephanie Humblet-Baron Stephanie Humblet-Baron Adrian Liston Adrian Liston Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations Frontiers in Immunology Nup210 CD4-positive T-lymphocytes CD8-positive T-lymphocytes thymus gland spleen nucleopore |
| title | Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations |
| title_full | Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations |
| title_fullStr | Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations |
| title_full_unstemmed | Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations |
| title_short | Mice Deficient in Nucleoporin Nup210 Develop Peripheral T Cell Alterations |
| title_sort | mice deficient in nucleoporin nup210 develop peripheral t cell alterations |
| topic | Nup210 CD4-positive T-lymphocytes CD8-positive T-lymphocytes thymus gland spleen nucleopore |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2018.02234/full |
| work_keys_str_mv | AT annemarievannieuwenhuijze micedeficientinnucleoporinnup210developperipheraltcellalterations AT annemarievannieuwenhuijze micedeficientinnucleoporinnup210developperipheraltcellalterations AT oliverburton micedeficientinnucleoporinnup210developperipheraltcellalterations AT oliverburton micedeficientinnucleoporinnup210developperipheraltcellalterations AT pierrelemaitre micedeficientinnucleoporinnup210developperipheraltcellalterations AT pierrelemaitre micedeficientinnucleoporinnup210developperipheraltcellalterations AT aliceedenton micedeficientinnucleoporinnup210developperipheraltcellalterations AT anacascalho micedeficientinnucleoporinnup210developperipheraltcellalterations AT anacascalho micedeficientinnucleoporinnup210developperipheraltcellalterations AT roseegoodchild micedeficientinnucleoporinnup210developperipheraltcellalterations AT roseegoodchild micedeficientinnucleoporinnup210developperipheraltcellalterations AT bertmalengierdevlies micedeficientinnucleoporinnup210developperipheraltcellalterations AT bertmalengierdevlies micedeficientinnucleoporinnup210developperipheraltcellalterations AT benedictecauwe micedeficientinnucleoporinnup210developperipheraltcellalterations AT benedictecauwe micedeficientinnucleoporinnup210developperipheraltcellalterations AT michellealinterman micedeficientinnucleoporinnup210developperipheraltcellalterations AT stephaniehumbletbaron micedeficientinnucleoporinnup210developperipheraltcellalterations AT stephaniehumbletbaron micedeficientinnucleoporinnup210developperipheraltcellalterations AT adrianliston micedeficientinnucleoporinnup210developperipheraltcellalterations AT adrianliston micedeficientinnucleoporinnup210developperipheraltcellalterations |