Endothelial loss during the surgical procedure in saphenous veins harvested by open and endoscopic techniques in coronary artery bypass surgery
The patency of the vein graft in coronary artery bypass grafting could be dependent on the conventional open (vsO) or endoscopic (vsE) harvesting and on the hypoxic damage of endothelial cells. We aimed to evaluate both surgical techniques according to endothelial loss that occurs in the time betwe...
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| Format: | Article |
| Language: | English |
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Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
2020-11-01
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| Series: | Biomolecules & Biomedicine |
| Subjects: | |
| Online Access: | https://www.bjbms.org/ojs/index.php/bjbms/article/view/4656 |
| _version_ | 1797260971988746240 |
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| author | Aleksandra Milutinović Ruda Zorc-Pleskovič |
| author_facet | Aleksandra Milutinović Ruda Zorc-Pleskovič |
| author_sort | Aleksandra Milutinović |
| collection | DOAJ |
| description |
The patency of the vein graft in coronary artery bypass grafting could be dependent on the conventional open (vsO) or endoscopic (vsE) harvesting and on the hypoxic damage of endothelial cells. We aimed to evaluate both surgical techniques according to endothelial loss that occurs in the time between harvesting and implantation. Twenty-six saphenous veins were divided into vsO (n = 16) and vsE (n = 10) group. Three samples were taken from each vein. The first sample was taken after removal, the second before implantation of the distal part, and the third before the implantation of the proximal part, and they were stained with HE, Movat, and immunohistochemically with CD31. A significant loss of endothelial cells within both groups was found at the time of implantation of the distal and the proximal part of the vein graft compared to the endothelial cells at the time of harvesting. There were no significant differences in the endothelial loss between vsE and vsO groups at the time of harvesting and at the time before the implantation of the distal part. A higher number of endothelial cells was found in vsE group compared to vsO group at the time just before the implantation of the proximal part. The comparison of the implanted portions of vsE and vsO grafts to mammary arteries revealed a significant loss of endothelial cells only in vsO graft. We conclude that, at the time of implantation, the endothelial layer of the vein graft harvested endoscopically is more preserved than of the vein graft harvested openly.
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| first_indexed | 2024-04-24T23:33:48Z |
| format | Article |
| id | doaj.art-ea6f5b3345944ff9b81a71a04cfd1a92 |
| institution | Directory Open Access Journal |
| issn | 2831-0896 2831-090X |
| language | English |
| last_indexed | 2024-04-24T23:33:48Z |
| publishDate | 2020-11-01 |
| publisher | Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina |
| record_format | Article |
| series | Biomolecules & Biomedicine |
| spelling | doaj.art-ea6f5b3345944ff9b81a71a04cfd1a922024-03-15T14:29:55ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBiomolecules & Biomedicine2831-08962831-090X2020-11-0120410.17305/bjbms.2020.4656Endothelial loss during the surgical procedure in saphenous veins harvested by open and endoscopic techniques in coronary artery bypass surgeryAleksandra Milutinović0https://orcid.org/0000-0003-4142-4814Ruda Zorc-Pleskovič1https://orcid.org/0000-0001-8208-4663Institute of Histology and Embryology, Faculty of Medicine, University of Ljubljana, Ljubljana, SloveniaInstitute of Histology and Embryology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia; International Center for Cardiovascular Diseases MC Medicor d.d., Izola, Slovenia The patency of the vein graft in coronary artery bypass grafting could be dependent on the conventional open (vsO) or endoscopic (vsE) harvesting and on the hypoxic damage of endothelial cells. We aimed to evaluate both surgical techniques according to endothelial loss that occurs in the time between harvesting and implantation. Twenty-six saphenous veins were divided into vsO (n = 16) and vsE (n = 10) group. Three samples were taken from each vein. The first sample was taken after removal, the second before implantation of the distal part, and the third before the implantation of the proximal part, and they were stained with HE, Movat, and immunohistochemically with CD31. A significant loss of endothelial cells within both groups was found at the time of implantation of the distal and the proximal part of the vein graft compared to the endothelial cells at the time of harvesting. There were no significant differences in the endothelial loss between vsE and vsO groups at the time of harvesting and at the time before the implantation of the distal part. A higher number of endothelial cells was found in vsE group compared to vsO group at the time just before the implantation of the proximal part. The comparison of the implanted portions of vsE and vsO grafts to mammary arteries revealed a significant loss of endothelial cells only in vsO graft. We conclude that, at the time of implantation, the endothelial layer of the vein graft harvested endoscopically is more preserved than of the vein graft harvested openly. https://www.bjbms.org/ojs/index.php/bjbms/article/view/4656Endothelial cellssaphenous veinendoscopic harvestingopen harvestingcoronary artery bypass grafting |
| spellingShingle | Aleksandra Milutinović Ruda Zorc-Pleskovič Endothelial loss during the surgical procedure in saphenous veins harvested by open and endoscopic techniques in coronary artery bypass surgery Biomolecules & Biomedicine Endothelial cells saphenous vein endoscopic harvesting open harvesting coronary artery bypass grafting |
| title | Endothelial loss during the surgical procedure in saphenous veins harvested by open and endoscopic techniques in coronary artery bypass surgery |
| title_full | Endothelial loss during the surgical procedure in saphenous veins harvested by open and endoscopic techniques in coronary artery bypass surgery |
| title_fullStr | Endothelial loss during the surgical procedure in saphenous veins harvested by open and endoscopic techniques in coronary artery bypass surgery |
| title_full_unstemmed | Endothelial loss during the surgical procedure in saphenous veins harvested by open and endoscopic techniques in coronary artery bypass surgery |
| title_short | Endothelial loss during the surgical procedure in saphenous veins harvested by open and endoscopic techniques in coronary artery bypass surgery |
| title_sort | endothelial loss during the surgical procedure in saphenous veins harvested by open and endoscopic techniques in coronary artery bypass surgery |
| topic | Endothelial cells saphenous vein endoscopic harvesting open harvesting coronary artery bypass grafting |
| url | https://www.bjbms.org/ojs/index.php/bjbms/article/view/4656 |
| work_keys_str_mv | AT aleksandramilutinovic endotheliallossduringthesurgicalprocedureinsaphenousveinsharvestedbyopenandendoscopictechniquesincoronaryarterybypasssurgery AT rudazorcpleskovic endotheliallossduringthesurgicalprocedureinsaphenousveinsharvestedbyopenandendoscopictechniquesincoronaryarterybypasssurgery |