Spatial Variations of Aquatic Bacterial Community Structure and Co-Occurrence Patterns in a Coal Mining Subsidence Lake
Land subsidence caused by underground coal mining critically influences the structure and function of ecosystems in mining areas. However, knowledge on the aquatic bacterial community structure and interspecies interactions in subsidence lakes are still limited. To address this issue, we collected w...
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MDPI AG
2022-08-01
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| author | Tingyu Fan Wangkai Fang Yifan Zhao Akang Lu Shun Wang Xingming Wang Liangji Xu Xiangping Wei Lei Zhang |
| author_facet | Tingyu Fan Wangkai Fang Yifan Zhao Akang Lu Shun Wang Xingming Wang Liangji Xu Xiangping Wei Lei Zhang |
| author_sort | Tingyu Fan |
| collection | DOAJ |
| description | Land subsidence caused by underground coal mining critically influences the structure and function of ecosystems in mining areas. However, knowledge on the aquatic bacterial community structure and interspecies interactions in subsidence lakes are still limited. To address this issue, we collected water samples from a coal mining subsidence lake and its connected river and investigated the spatial distribution and co-occurrence patterns of the bacterial community using 16S rRNA amplicon sequencing. The results revealed that the bacterial α-diversity in the subsidence lake was higher than that in the river. The bacterial community composition was also significantly different between the subsidence lake and its connected river. Total nitrogen explained 21.4% of the bacterial community composition variation, while sulfate explained 38.4% of the bacterial functional composition variation. Co-occurrence network analysis indicated that the modularity indices and stability of the microbial network in the subsidence lake were significantly higher than those in rivers, which presented more resistance to environmental disturbance. Keystone bacterial taxa in the subsidence lake and river included the <i>Clostridiaceae 1</i> family, and the <i>Shewanella</i>, <i>Flavobacterium</i>, and <i>Limnohabitans</i> genera, which play vital roles in the carbon, sulfur, and nitrogen cycles. Moreover, functional analysis showed that assimilatory sulfate reduction processes had a major role in the sulfur cycle of the subsidence lake and its connected river ecosystem. Overall, our findings provide new insights into the microbial community structure and assembly in subsidence lakes and its connected river ecosystems, with significant implications for the responsible utilization of water resources and the promotion of sustainable development in mining areas. |
| first_indexed | 2024-03-09T09:58:51Z |
| format | Article |
| id | doaj.art-ea714a3c78864ca68c848edfc107b014 |
| institution | Directory Open Access Journal |
| issn | 1424-2818 |
| language | English |
| last_indexed | 2024-03-09T09:58:51Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diversity |
| spelling | doaj.art-ea714a3c78864ca68c848edfc107b0142023-12-01T23:37:26ZengMDPI AGDiversity1424-28182022-08-0114867410.3390/d14080674Spatial Variations of Aquatic Bacterial Community Structure and Co-Occurrence Patterns in a Coal Mining Subsidence LakeTingyu Fan0Wangkai Fang1Yifan Zhao2Akang Lu3Shun Wang4Xingming Wang5Liangji Xu6Xiangping Wei7Lei Zhang8School of Earth and Environment, Anhui University of Science and Technology, Huainan 232001, ChinaSchool of Earth and Environment, Anhui University of Science and Technology, Huainan 232001, ChinaSchool of Earth and Environment, Anhui University of Science and Technology, Huainan 232001, ChinaSchool of Earth and Environment, Anhui University of Science and Technology, Huainan 232001, ChinaSchool of Earth and Environment, Anhui University of Science and Technology, Huainan 232001, ChinaSchool of Earth and Environment, Anhui University of Science and Technology, Huainan 232001, ChinaSchool of Earth and Environment, Anhui University of Science and Technology, Huainan 232001, ChinaHuaibei Mining Group, Huaibei 235000, ChinaSchool of Civil Engineering and Architecture, Chuzhou University, Chuzhou 239000, ChinaLand subsidence caused by underground coal mining critically influences the structure and function of ecosystems in mining areas. However, knowledge on the aquatic bacterial community structure and interspecies interactions in subsidence lakes are still limited. To address this issue, we collected water samples from a coal mining subsidence lake and its connected river and investigated the spatial distribution and co-occurrence patterns of the bacterial community using 16S rRNA amplicon sequencing. The results revealed that the bacterial α-diversity in the subsidence lake was higher than that in the river. The bacterial community composition was also significantly different between the subsidence lake and its connected river. Total nitrogen explained 21.4% of the bacterial community composition variation, while sulfate explained 38.4% of the bacterial functional composition variation. Co-occurrence network analysis indicated that the modularity indices and stability of the microbial network in the subsidence lake were significantly higher than those in rivers, which presented more resistance to environmental disturbance. Keystone bacterial taxa in the subsidence lake and river included the <i>Clostridiaceae 1</i> family, and the <i>Shewanella</i>, <i>Flavobacterium</i>, and <i>Limnohabitans</i> genera, which play vital roles in the carbon, sulfur, and nitrogen cycles. Moreover, functional analysis showed that assimilatory sulfate reduction processes had a major role in the sulfur cycle of the subsidence lake and its connected river ecosystem. Overall, our findings provide new insights into the microbial community structure and assembly in subsidence lakes and its connected river ecosystems, with significant implications for the responsible utilization of water resources and the promotion of sustainable development in mining areas.https://www.mdpi.com/1424-2818/14/8/674coal mining subsidence lakebacterial diversitybacterial community structureco-occurrence networksulfur cycle |
| spellingShingle | Tingyu Fan Wangkai Fang Yifan Zhao Akang Lu Shun Wang Xingming Wang Liangji Xu Xiangping Wei Lei Zhang Spatial Variations of Aquatic Bacterial Community Structure and Co-Occurrence Patterns in a Coal Mining Subsidence Lake Diversity coal mining subsidence lake bacterial diversity bacterial community structure co-occurrence network sulfur cycle |
| title | Spatial Variations of Aquatic Bacterial Community Structure and Co-Occurrence Patterns in a Coal Mining Subsidence Lake |
| title_full | Spatial Variations of Aquatic Bacterial Community Structure and Co-Occurrence Patterns in a Coal Mining Subsidence Lake |
| title_fullStr | Spatial Variations of Aquatic Bacterial Community Structure and Co-Occurrence Patterns in a Coal Mining Subsidence Lake |
| title_full_unstemmed | Spatial Variations of Aquatic Bacterial Community Structure and Co-Occurrence Patterns in a Coal Mining Subsidence Lake |
| title_short | Spatial Variations of Aquatic Bacterial Community Structure and Co-Occurrence Patterns in a Coal Mining Subsidence Lake |
| title_sort | spatial variations of aquatic bacterial community structure and co occurrence patterns in a coal mining subsidence lake |
| topic | coal mining subsidence lake bacterial diversity bacterial community structure co-occurrence network sulfur cycle |
| url | https://www.mdpi.com/1424-2818/14/8/674 |
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