| Summary: | The study was conducted to screen high 1,2-propanediol produced by <i>Lactobacillus buchneri</i> strains, isolated from baled silages stored for 1 or 2 years, and to evaluate their effects on fermentation quality and aerobic stability of whole-plant corn silage. In total, 31 <i>L. buchneri strains</i> were isolated from alfalfa, whole-plant corn and oat silages. Based on growth performance and 1,2-propanediol and acetic acid production, two strains, <i>L. buchneri</i> 9-2 and <i>L. buchneri</i> 10-1, from alfalfa silage, were further assessed in an ensiling trial on whole-plant corn. The corn silage inoculated with <i>L. buchneri</i> 9-2 or <i>L. buchneri</i> 10-1 had a higher concentration of 1,2-propanediol (34.7 or 34.6 g/kg dry matter (DM)) and acetic acid (47.2 or 45.9 g/kg DM) in comparison with <i>L. buchneri</i> 40788 (reference strain) treated silage (19.5 and 35.9 g/kg DM) after 90 d of fermentation. In addition, these two strains performed better in improving silage aerobic stability relative to control and <i>L. buchneri</i> 40788. The results above indicated that <i>L. buchneri</i> 9-2 and <i>L. buchneri</i> 10-1 could be candidate strains to increase 1,2-propanediol and acetic acid concentrations and improve the aerobic stability of whole-plant corn silage.
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