Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review

The low grade ejaculates are very common in bulls. Low grade ejaculates might be due to age or non specific factors like thermal stress, transport and vaccination stress during the dynamic life of bulls. Lipid peroxidation of membrane induced sperm damage further aggravates the situation. Researches...

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Main Authors: Shailendra Chaurasia, Indrasen Chauhan, Navinkumar B. Patel, Thakur Krishna Shankar Rao, Niranjan Kumar
Format: Article
Language:English
Published: Veterinary World 2013-06-01
Series:Veterinary World
Subjects:
Online Access:http://www.veterinaryworld.org/Vol.6/August - 2013/Sperm selection techniques and antioxidant fortification in low grade semen of bulls.pdf
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author Shailendra Chaurasia
Indrasen Chauhan
Navinkumar B. Patel
Thakur Krishna Shankar Rao
Niranjan Kumar
author_facet Shailendra Chaurasia
Indrasen Chauhan
Navinkumar B. Patel
Thakur Krishna Shankar Rao
Niranjan Kumar
author_sort Shailendra Chaurasia
collection DOAJ
description The low grade ejaculates are very common in bulls. Low grade ejaculates might be due to age or non specific factors like thermal stress, transport and vaccination stress during the dynamic life of bulls. Lipid peroxidation of membrane induced sperm damage further aggravates the situation. Researches reveal that selection of sperm and antioxidant fortification play crucial role in improving the quality of semen. Different methods used for semen up-gradation like washing, sedimentation, swim up procedure and filtration like percoll gradient, glass wool, sephadex and sephadex ion exchanger with significant improvement in motility, Hypo osmotic swelling test reactivity, viability and acrosomal integrity. Antioxidants are added directly to extender at standard dose rate with positive result. Among different filtration column used sephadex ion exchanger (FS+IE) was superior over other in improving the semen quality especially when fortified with Vitamin E. Moreover a complete protocol is required, which contain both antioxidant fortification and sperm selection simultaneously to handle the low grade semen from sub-fertile bulls. [Vet World 2013; 6(8.000): 579-585]
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spelling doaj.art-ea763b076f7b4020a522c344d864c7782022-12-21T22:15:29ZengVeterinary WorldVeterinary World0972-89882231-09162013-06-016857958510.5455/vetworld.2013.579-585Sperm selection techniques and antioxidant fortification in low grade semen of bulls: ReviewShailendra ChaurasiaIndrasen ChauhanNavinkumar B. PatelThakur Krishna Shankar RaoNiranjan KumarThe low grade ejaculates are very common in bulls. Low grade ejaculates might be due to age or non specific factors like thermal stress, transport and vaccination stress during the dynamic life of bulls. Lipid peroxidation of membrane induced sperm damage further aggravates the situation. Researches reveal that selection of sperm and antioxidant fortification play crucial role in improving the quality of semen. Different methods used for semen up-gradation like washing, sedimentation, swim up procedure and filtration like percoll gradient, glass wool, sephadex and sephadex ion exchanger with significant improvement in motility, Hypo osmotic swelling test reactivity, viability and acrosomal integrity. Antioxidants are added directly to extender at standard dose rate with positive result. Among different filtration column used sephadex ion exchanger (FS+IE) was superior over other in improving the semen quality especially when fortified with Vitamin E. Moreover a complete protocol is required, which contain both antioxidant fortification and sperm selection simultaneously to handle the low grade semen from sub-fertile bulls. [Vet World 2013; 6(8.000): 579-585]http://www.veterinaryworld.org/Vol.6/August - 2013/Sperm selection techniques and antioxidant fortification in low grade semen of bulls.pdfantioxidantbullgeneticsemensperm
spellingShingle Shailendra Chaurasia
Indrasen Chauhan
Navinkumar B. Patel
Thakur Krishna Shankar Rao
Niranjan Kumar
Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review
Veterinary World
antioxidant
bull
genetic
semen
sperm
title Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review
title_full Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review
title_fullStr Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review
title_full_unstemmed Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review
title_short Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review
title_sort sperm selection techniques and antioxidant fortification in low grade semen of bulls review
topic antioxidant
bull
genetic
semen
sperm
url http://www.veterinaryworld.org/Vol.6/August - 2013/Sperm selection techniques and antioxidant fortification in low grade semen of bulls.pdf
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