Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review
The low grade ejaculates are very common in bulls. Low grade ejaculates might be due to age or non specific factors like thermal stress, transport and vaccination stress during the dynamic life of bulls. Lipid peroxidation of membrane induced sperm damage further aggravates the situation. Researches...
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Format: | Article |
Language: | English |
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Veterinary World
2013-06-01
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Series: | Veterinary World |
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Online Access: | http://www.veterinaryworld.org/Vol.6/August - 2013/Sperm selection techniques and antioxidant fortification in low grade semen of bulls.pdf |
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author | Shailendra Chaurasia Indrasen Chauhan Navinkumar B. Patel Thakur Krishna Shankar Rao Niranjan Kumar |
author_facet | Shailendra Chaurasia Indrasen Chauhan Navinkumar B. Patel Thakur Krishna Shankar Rao Niranjan Kumar |
author_sort | Shailendra Chaurasia |
collection | DOAJ |
description | The low grade ejaculates are very common in bulls. Low grade ejaculates might be due to age or non specific factors like thermal stress, transport and vaccination stress during the dynamic life of bulls. Lipid peroxidation of membrane induced sperm damage further aggravates the situation. Researches reveal that selection of sperm and antioxidant fortification play crucial role in improving the quality of semen. Different methods used for semen up-gradation like washing, sedimentation, swim up procedure and filtration like percoll gradient, glass wool, sephadex and sephadex ion exchanger with significant improvement in motility, Hypo osmotic swelling test reactivity, viability and acrosomal integrity. Antioxidants are added directly to extender at standard dose rate with positive result. Among different filtration column used sephadex ion exchanger (FS+IE) was superior over other in improving the semen quality especially when fortified with Vitamin E. Moreover a complete protocol is required, which contain both antioxidant fortification and sperm selection simultaneously to handle the low grade semen from sub-fertile bulls. [Vet World 2013; 6(8.000): 579-585] |
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institution | Directory Open Access Journal |
issn | 0972-8988 2231-0916 |
language | English |
last_indexed | 2024-12-16T21:33:16Z |
publishDate | 2013-06-01 |
publisher | Veterinary World |
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series | Veterinary World |
spelling | doaj.art-ea763b076f7b4020a522c344d864c7782022-12-21T22:15:29ZengVeterinary WorldVeterinary World0972-89882231-09162013-06-016857958510.5455/vetworld.2013.579-585Sperm selection techniques and antioxidant fortification in low grade semen of bulls: ReviewShailendra ChaurasiaIndrasen ChauhanNavinkumar B. PatelThakur Krishna Shankar RaoNiranjan KumarThe low grade ejaculates are very common in bulls. Low grade ejaculates might be due to age or non specific factors like thermal stress, transport and vaccination stress during the dynamic life of bulls. Lipid peroxidation of membrane induced sperm damage further aggravates the situation. Researches reveal that selection of sperm and antioxidant fortification play crucial role in improving the quality of semen. Different methods used for semen up-gradation like washing, sedimentation, swim up procedure and filtration like percoll gradient, glass wool, sephadex and sephadex ion exchanger with significant improvement in motility, Hypo osmotic swelling test reactivity, viability and acrosomal integrity. Antioxidants are added directly to extender at standard dose rate with positive result. Among different filtration column used sephadex ion exchanger (FS+IE) was superior over other in improving the semen quality especially when fortified with Vitamin E. Moreover a complete protocol is required, which contain both antioxidant fortification and sperm selection simultaneously to handle the low grade semen from sub-fertile bulls. [Vet World 2013; 6(8.000): 579-585]http://www.veterinaryworld.org/Vol.6/August - 2013/Sperm selection techniques and antioxidant fortification in low grade semen of bulls.pdfantioxidantbullgeneticsemensperm |
spellingShingle | Shailendra Chaurasia Indrasen Chauhan Navinkumar B. Patel Thakur Krishna Shankar Rao Niranjan Kumar Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review Veterinary World antioxidant bull genetic semen sperm |
title | Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review |
title_full | Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review |
title_fullStr | Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review |
title_full_unstemmed | Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review |
title_short | Sperm selection techniques and antioxidant fortification in low grade semen of bulls: Review |
title_sort | sperm selection techniques and antioxidant fortification in low grade semen of bulls review |
topic | antioxidant bull genetic semen sperm |
url | http://www.veterinaryworld.org/Vol.6/August - 2013/Sperm selection techniques and antioxidant fortification in low grade semen of bulls.pdf |
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