Porphyromonas gingivalis Infection Induces Lipopolysaccharide and Peptidoglycan Penetration Through Gingival Epithelium
Periodontal diseases initiate on epithelial surfaces of the subgingival compartment, while the gingival epithelium functions as an epithelial barrier against microbial infection and orchestrates immune responses. Porphyromonas gingivalis is a major pathogen of periodontal diseases and has an ability...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Oral Health |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/froh.2022.845002/full |
| _version_ | 1818285727937462272 |
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| author | Hiroki Takeuchi Eriko Nakamura Shunsuke Yamaga Atsuo Amano |
| author_facet | Hiroki Takeuchi Eriko Nakamura Shunsuke Yamaga Atsuo Amano |
| author_sort | Hiroki Takeuchi |
| collection | DOAJ |
| description | Periodontal diseases initiate on epithelial surfaces of the subgingival compartment, while the gingival epithelium functions as an epithelial barrier against microbial infection and orchestrates immune responses. Porphyromonas gingivalis is a major pathogen of periodontal diseases and has an ability to penetrate the epithelial barrier. To assess the molecular basis of gingival epithelial barrier dysfunction associated with P. gingivalis, we newly developed a three-dimensional multilayered tissue model of gingival epithelium with gene manipulation. Using this novel approach, P. gingivalis gingipains including Arg- or Lys-specific cysteine proteases were found to specifically degrade junctional adhesion molecule 1 and coxsackievirus and adenovirus receptor in the tissue model, leading to increased permeability for lipopolysaccharide, peptidoglycan, and gingipains. This review summarizes the strategy used by P. gingivalis to disable the epithelial barrier by disrupting specific junctional adhesion molecules. |
| first_indexed | 2024-12-13T01:13:18Z |
| format | Article |
| id | doaj.art-ea78f59fc5ad4fd48224033335a9361d |
| institution | Directory Open Access Journal |
| issn | 2673-4842 |
| language | English |
| last_indexed | 2024-12-13T01:13:18Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oral Health |
| spelling | doaj.art-ea78f59fc5ad4fd48224033335a9361d2022-12-22T00:04:24ZengFrontiers Media S.A.Frontiers in Oral Health2673-48422022-02-01310.3389/froh.2022.845002845002Porphyromonas gingivalis Infection Induces Lipopolysaccharide and Peptidoglycan Penetration Through Gingival EpitheliumHiroki Takeuchi0Eriko Nakamura1Shunsuke Yamaga2Atsuo Amano3Department of Preventive Dentistry, Osaka University Dental Hospital, Suita, JapanDepartment of Preventive Dentistry, Graduate School of Dentistry, Osaka University, Suita, JapanDepartment of Preventive Dentistry, Graduate School of Dentistry, Osaka University, Suita, JapanDepartment of Preventive Dentistry, Graduate School of Dentistry, Osaka University, Suita, JapanPeriodontal diseases initiate on epithelial surfaces of the subgingival compartment, while the gingival epithelium functions as an epithelial barrier against microbial infection and orchestrates immune responses. Porphyromonas gingivalis is a major pathogen of periodontal diseases and has an ability to penetrate the epithelial barrier. To assess the molecular basis of gingival epithelial barrier dysfunction associated with P. gingivalis, we newly developed a three-dimensional multilayered tissue model of gingival epithelium with gene manipulation. Using this novel approach, P. gingivalis gingipains including Arg- or Lys-specific cysteine proteases were found to specifically degrade junctional adhesion molecule 1 and coxsackievirus and adenovirus receptor in the tissue model, leading to increased permeability for lipopolysaccharide, peptidoglycan, and gingipains. This review summarizes the strategy used by P. gingivalis to disable the epithelial barrier by disrupting specific junctional adhesion molecules.https://www.frontiersin.org/articles/10.3389/froh.2022.845002/fullperiodontitisPorphyromonas gingivalisgingipainsgingival epitheliumlipopolysaccharidepeptidoglycan |
| spellingShingle | Hiroki Takeuchi Eriko Nakamura Shunsuke Yamaga Atsuo Amano Porphyromonas gingivalis Infection Induces Lipopolysaccharide and Peptidoglycan Penetration Through Gingival Epithelium Frontiers in Oral Health periodontitis Porphyromonas gingivalis gingipains gingival epithelium lipopolysaccharide peptidoglycan |
| title | Porphyromonas gingivalis Infection Induces Lipopolysaccharide and Peptidoglycan Penetration Through Gingival Epithelium |
| title_full | Porphyromonas gingivalis Infection Induces Lipopolysaccharide and Peptidoglycan Penetration Through Gingival Epithelium |
| title_fullStr | Porphyromonas gingivalis Infection Induces Lipopolysaccharide and Peptidoglycan Penetration Through Gingival Epithelium |
| title_full_unstemmed | Porphyromonas gingivalis Infection Induces Lipopolysaccharide and Peptidoglycan Penetration Through Gingival Epithelium |
| title_short | Porphyromonas gingivalis Infection Induces Lipopolysaccharide and Peptidoglycan Penetration Through Gingival Epithelium |
| title_sort | porphyromonas gingivalis infection induces lipopolysaccharide and peptidoglycan penetration through gingival epithelium |
| topic | periodontitis Porphyromonas gingivalis gingipains gingival epithelium lipopolysaccharide peptidoglycan |
| url | https://www.frontiersin.org/articles/10.3389/froh.2022.845002/full |
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