The alteration of the structure and macroscopic mechanical response of porcine patellar tendon by elastase digestion
Background: The treatment of patellar tendon injury has always been an unsolved problem, and mechanical characterization is very important for its repair and reconstruction. Elastin is a contributor to mechanics, but it is not clear how it affects the elasticity, viscoelastic properties, and structu...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-04-01
|
| Series: | Frontiers in Bioengineering and Biotechnology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1374352/full |
| _version_ | 1827279214724775936 |
|---|---|
| author | Xiaoyun Liu Xiaoyun Liu Yuping Deng Yuping Deng Yuping Deng Zeyu Liang Dan Qiao Wentian Zhang Wentian Zhang Mian Wang Mian Wang Mian Wang Feifei Li Jiannan Liu Yaobing Wu Guangxin Chen Yan Liu Wenchang Tan Jian Xing Wenhua Huang Wenhua Huang Wenhua Huang Dongliang Zhao Yanbing Li |
| author_facet | Xiaoyun Liu Xiaoyun Liu Yuping Deng Yuping Deng Yuping Deng Zeyu Liang Dan Qiao Wentian Zhang Wentian Zhang Mian Wang Mian Wang Mian Wang Feifei Li Jiannan Liu Yaobing Wu Guangxin Chen Yan Liu Wenchang Tan Jian Xing Wenhua Huang Wenhua Huang Wenhua Huang Dongliang Zhao Yanbing Li |
| author_sort | Xiaoyun Liu |
| collection | DOAJ |
| description | Background: The treatment of patellar tendon injury has always been an unsolved problem, and mechanical characterization is very important for its repair and reconstruction. Elastin is a contributor to mechanics, but it is not clear how it affects the elasticity, viscoelastic properties, and structure of patellar tendon.Methods: The patellar tendons from six fresh adult experimental pigs were used in this study and they were made into 77 samples. The patellar tendon was specifically degraded by elastase, and the regional mechanical response and structural changes were investigated by: (1) Based on the previous study of elastase treatment conditions, the biochemical quantification of collagen, glycosaminoglycan and total protein was carried out; (2) The patellar tendon was divided into the proximal, central, and distal regions, and then the axial tensile test and stress relaxation test were performed before and after phosphate-buffered saline (PBS) or elastase treatment; (3) The dynamic constitutive model was established by the obtained mechanical data; (4) The structural relationship between elastin and collagen fibers was analyzed by two-photon microscopy and histology.Results: There was no statistical difference in mechanics between patellar tendon regions. Compared with those before elastase treatment, the low tensile modulus decreased by 75%–80%, the high tensile modulus decreased by 38%–47%, and the transition strain was prolonged after treatment. For viscoelastic behavior, the stress relaxation increased, the initial slope increased by 55%, the saturation slope increased by 44%, and the transition time increased by 25% after enzyme treatment. Elastin degradation made the collagen fibers of patellar tendon become disordered and looser, and the fiber wavelength increased significantly.Conclusion: The results of this study show that elastin plays an important role in the mechanical properties and fiber structure stability of patellar tendon, which supplements the structure-function relationship information of patellar tendon. The established constitutive model is of great significance to the prediction, repair and replacement of patellar tendon injury. In addition, human patellar tendon has a higher elastin content, so the results of this study can provide supporting information on the natural properties of tendon elastin degradation and guide the development of artificial patellar tendon biomaterials. |
| first_indexed | 2024-04-24T08:15:32Z |
| format | Article |
| id | doaj.art-ea7ca44565e945e795c6fedb38dab5db |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-24T08:15:32Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-ea7ca44565e945e795c6fedb38dab5db2024-04-17T04:16:12ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852024-04-011210.3389/fbioe.2024.13743521374352The alteration of the structure and macroscopic mechanical response of porcine patellar tendon by elastase digestionXiaoyun Liu0Xiaoyun Liu1Yuping Deng2Yuping Deng3Yuping Deng4Zeyu Liang5Dan Qiao6Wentian Zhang7Wentian Zhang8Mian Wang9Mian Wang10Mian Wang11Feifei Li12Jiannan Liu13Yaobing Wu14Guangxin Chen15Yan Liu16Wenchang Tan17Jian Xing18Wenhua Huang19Wenhua Huang20Wenhua Huang21Dongliang Zhao22Yanbing Li23National Key Discipline of Human Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaInstitute of Biomedical Engineering, Shenzhen Bay Laboratory, Peking University Shenzhen Graduate School, Shenzhen, ChinaNational Key Discipline of Human Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaInstitute of Biomedical Engineering, Shenzhen Bay Laboratory, Peking University Shenzhen Graduate School, Shenzhen, ChinaDepartment of Orthopedics and Traumatology, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou, ChinaNational Key Discipline of Human Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaDepartment of Pathology, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, Zhejiang, ChinaNational Key Discipline of Human Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaThe School of Basic Medical Sciences, Fujian Medical University, Fujian, ChinaNational Key Discipline of Human Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaInstitute of Biomedical Engineering, Shenzhen Bay Laboratory, Peking University Shenzhen Graduate School, Shenzhen, ChinaDepartment of Orthopaedics, Pingshan General Hospital of Southern Medical University, Shenzhen, ChinaNational Key Discipline of Human Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaNational Key Discipline of Human Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaNational Key Discipline of Human Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaMedical Image College, Mudanjiang Medical University, Mudanjiang, Heilongjiang, ChinaDepartment of Anatomy, Gannan Healthcare Vocational College, Ganzhou, ChinaInstitute of Biomedical Engineering, Shenzhen Bay Laboratory, Peking University Shenzhen Graduate School, Shenzhen, ChinaMedical Image College, Mudanjiang Medical University, Mudanjiang, Heilongjiang, ChinaNational Key Discipline of Human Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaDepartment of Orthopedics and Traumatology, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou, ChinaGuangdong Medical Innovation Platform for Translation of 3D Printing Application, The Third Affiliated Hospital of Southern Medical University, Guangzhou, ChinaInstitute of Biomedical Engineering, Shenzhen Bay Laboratory, Peking University Shenzhen Graduate School, Shenzhen, ChinaNational Key Discipline of Human Anatomy, Guangdong Provincial Key Laboratory of Medical Biomechanics, Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaBackground: The treatment of patellar tendon injury has always been an unsolved problem, and mechanical characterization is very important for its repair and reconstruction. Elastin is a contributor to mechanics, but it is not clear how it affects the elasticity, viscoelastic properties, and structure of patellar tendon.Methods: The patellar tendons from six fresh adult experimental pigs were used in this study and they were made into 77 samples. The patellar tendon was specifically degraded by elastase, and the regional mechanical response and structural changes were investigated by: (1) Based on the previous study of elastase treatment conditions, the biochemical quantification of collagen, glycosaminoglycan and total protein was carried out; (2) The patellar tendon was divided into the proximal, central, and distal regions, and then the axial tensile test and stress relaxation test were performed before and after phosphate-buffered saline (PBS) or elastase treatment; (3) The dynamic constitutive model was established by the obtained mechanical data; (4) The structural relationship between elastin and collagen fibers was analyzed by two-photon microscopy and histology.Results: There was no statistical difference in mechanics between patellar tendon regions. Compared with those before elastase treatment, the low tensile modulus decreased by 75%–80%, the high tensile modulus decreased by 38%–47%, and the transition strain was prolonged after treatment. For viscoelastic behavior, the stress relaxation increased, the initial slope increased by 55%, the saturation slope increased by 44%, and the transition time increased by 25% after enzyme treatment. Elastin degradation made the collagen fibers of patellar tendon become disordered and looser, and the fiber wavelength increased significantly.Conclusion: The results of this study show that elastin plays an important role in the mechanical properties and fiber structure stability of patellar tendon, which supplements the structure-function relationship information of patellar tendon. The established constitutive model is of great significance to the prediction, repair and replacement of patellar tendon injury. In addition, human patellar tendon has a higher elastin content, so the results of this study can provide supporting information on the natural properties of tendon elastin degradation and guide the development of artificial patellar tendon biomaterials.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1374352/fullpatellar tendonelastinnonlinearitystress relaxationtwo-photon imaging |
| spellingShingle | Xiaoyun Liu Xiaoyun Liu Yuping Deng Yuping Deng Yuping Deng Zeyu Liang Dan Qiao Wentian Zhang Wentian Zhang Mian Wang Mian Wang Mian Wang Feifei Li Jiannan Liu Yaobing Wu Guangxin Chen Yan Liu Wenchang Tan Jian Xing Wenhua Huang Wenhua Huang Wenhua Huang Dongliang Zhao Yanbing Li The alteration of the structure and macroscopic mechanical response of porcine patellar tendon by elastase digestion Frontiers in Bioengineering and Biotechnology patellar tendon elastin nonlinearity stress relaxation two-photon imaging |
| title | The alteration of the structure and macroscopic mechanical response of porcine patellar tendon by elastase digestion |
| title_full | The alteration of the structure and macroscopic mechanical response of porcine patellar tendon by elastase digestion |
| title_fullStr | The alteration of the structure and macroscopic mechanical response of porcine patellar tendon by elastase digestion |
| title_full_unstemmed | The alteration of the structure and macroscopic mechanical response of porcine patellar tendon by elastase digestion |
| title_short | The alteration of the structure and macroscopic mechanical response of porcine patellar tendon by elastase digestion |
| title_sort | alteration of the structure and macroscopic mechanical response of porcine patellar tendon by elastase digestion |
| topic | patellar tendon elastin nonlinearity stress relaxation two-photon imaging |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1374352/full |
| work_keys_str_mv | AT xiaoyunliu thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT xiaoyunliu thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT yupingdeng thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT yupingdeng thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT yupingdeng thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT zeyuliang thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT danqiao thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT wentianzhang thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT wentianzhang thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT mianwang thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT mianwang thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT mianwang thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT feifeili thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT jiannanliu thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT yaobingwu thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT guangxinchen thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT yanliu thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT wenchangtan thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT jianxing thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT wenhuahuang thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT wenhuahuang thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT wenhuahuang thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT dongliangzhao thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT yanbingli thealterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT xiaoyunliu alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT xiaoyunliu alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT yupingdeng alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT yupingdeng alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT yupingdeng alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT zeyuliang alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT danqiao alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT wentianzhang alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT wentianzhang alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT mianwang alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT mianwang alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT mianwang alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT feifeili alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT jiannanliu alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT yaobingwu alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT guangxinchen alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT yanliu alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT wenchangtan alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT jianxing alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT wenhuahuang alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT wenhuahuang alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT wenhuahuang alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT dongliangzhao alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion AT yanbingli alterationofthestructureandmacroscopicmechanicalresponseofporcinepatellartendonbyelastasedigestion |