Bicarbonate Effects on Antibacterial Immunity and Mucus Glycobiology in the Cystic Fibrosis Lung: A Review With Selected Experimental Observations
Abstract. The primary defect in cystic fibrosis (CF) is abnormal chloride and bicarbonate transport in the CF transmembrane conductance regulator epithelial ion channel. The apical surface of the respiratory tract is lined by an airway surface liquid (ASL) layer composed of mucin comprising mainly M...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Health
2022-09-01
|
| Series: | Infectious Microbes & Diseases |
| Online Access: | http://journals.lww.com/10.1097/IM9.0000000000000101 |
| _version_ | 1828107919797780480 |
|---|---|
| author | Ruth Siew Tzung-Lin Ou Samira Dahesh Kathryn Akong Victor Nizet |
| author_facet | Ruth Siew Tzung-Lin Ou Samira Dahesh Kathryn Akong Victor Nizet |
| author_sort | Ruth Siew |
| collection | DOAJ |
| description | Abstract. The primary defect in cystic fibrosis (CF) is abnormal chloride and bicarbonate transport in the CF transmembrane conductance regulator epithelial ion channel. The apical surface of the respiratory tract is lined by an airway surface liquid (ASL) layer composed of mucin comprising mainly MUC5A and MUC5B glycoproteins. ASL homeostasis depends on sodium bicarbonate secretion into the airways and secretion deficits alter mucus properties leading to airway obstruction, inflammation and infections. Downstream effects of abnormal ion transport in the lungs include altered intrinsic immune defenses. We observed that neutrophils killed Pseudomonas aeruginosa more efficiently when it had been exposed to sodium bicarbonate, and formation of neutrophil extracellular traps by neutrophils was augmented in the presence of increasing bicarbonate concentrations. Physiological levels of bicarbonate sensitized P. aeruginosa to the antimicrobial peptide cathelicidin LL-37, which is present in both lung ASL and neutrophil extracellular traps. Sodium bicarbonate has various uses in clinical medicine and in the care of CF patients and could be further explored as a therapeutic adjunct against Pseudomonas infections. |
| first_indexed | 2024-04-11T10:38:31Z |
| format | Article |
| id | doaj.art-ea7e13c8a93e4c2b829d32bc8a302866 |
| institution | Directory Open Access Journal |
| issn | 2641-5917 |
| language | English |
| last_indexed | 2024-04-11T10:38:31Z |
| publishDate | 2022-09-01 |
| publisher | Wolters Kluwer Health |
| record_format | Article |
| series | Infectious Microbes & Diseases |
| spelling | doaj.art-ea7e13c8a93e4c2b829d32bc8a3028662022-12-22T04:29:14ZengWolters Kluwer HealthInfectious Microbes & Diseases2641-59172022-09-014310311010.1097/IM9.0000000000000101202209000-00004Bicarbonate Effects on Antibacterial Immunity and Mucus Glycobiology in the Cystic Fibrosis Lung: A Review With Selected Experimental ObservationsRuth Siew0Tzung-Lin Ou1Samira Dahesh2Kathryn Akong3Victor Nizet4Author affiliations: 1 Department of Pediatrics, University of California San Diego, La Jolla, California, USA2 School of Medicine, China Medical University, Taichung, TaiwanAuthor affiliations: 1 Department of Pediatrics, University of California San Diego, La Jolla, California, USAAuthor affiliations: 1 Department of Pediatrics, University of California San Diego, La Jolla, California, USAAuthor affiliations: 1 Department of Pediatrics, University of California San Diego, La Jolla, California, USAAbstract. The primary defect in cystic fibrosis (CF) is abnormal chloride and bicarbonate transport in the CF transmembrane conductance regulator epithelial ion channel. The apical surface of the respiratory tract is lined by an airway surface liquid (ASL) layer composed of mucin comprising mainly MUC5A and MUC5B glycoproteins. ASL homeostasis depends on sodium bicarbonate secretion into the airways and secretion deficits alter mucus properties leading to airway obstruction, inflammation and infections. Downstream effects of abnormal ion transport in the lungs include altered intrinsic immune defenses. We observed that neutrophils killed Pseudomonas aeruginosa more efficiently when it had been exposed to sodium bicarbonate, and formation of neutrophil extracellular traps by neutrophils was augmented in the presence of increasing bicarbonate concentrations. Physiological levels of bicarbonate sensitized P. aeruginosa to the antimicrobial peptide cathelicidin LL-37, which is present in both lung ASL and neutrophil extracellular traps. Sodium bicarbonate has various uses in clinical medicine and in the care of CF patients and could be further explored as a therapeutic adjunct against Pseudomonas infections.http://journals.lww.com/10.1097/IM9.0000000000000101 |
| spellingShingle | Ruth Siew Tzung-Lin Ou Samira Dahesh Kathryn Akong Victor Nizet Bicarbonate Effects on Antibacterial Immunity and Mucus Glycobiology in the Cystic Fibrosis Lung: A Review With Selected Experimental Observations Infectious Microbes & Diseases |
| title | Bicarbonate Effects on Antibacterial Immunity and Mucus Glycobiology in the Cystic Fibrosis Lung: A Review With Selected Experimental Observations |
| title_full | Bicarbonate Effects on Antibacterial Immunity and Mucus Glycobiology in the Cystic Fibrosis Lung: A Review With Selected Experimental Observations |
| title_fullStr | Bicarbonate Effects on Antibacterial Immunity and Mucus Glycobiology in the Cystic Fibrosis Lung: A Review With Selected Experimental Observations |
| title_full_unstemmed | Bicarbonate Effects on Antibacterial Immunity and Mucus Glycobiology in the Cystic Fibrosis Lung: A Review With Selected Experimental Observations |
| title_short | Bicarbonate Effects on Antibacterial Immunity and Mucus Glycobiology in the Cystic Fibrosis Lung: A Review With Selected Experimental Observations |
| title_sort | bicarbonate effects on antibacterial immunity and mucus glycobiology in the cystic fibrosis lung a review with selected experimental observations |
| url | http://journals.lww.com/10.1097/IM9.0000000000000101 |
| work_keys_str_mv | AT ruthsiew bicarbonateeffectsonantibacterialimmunityandmucusglycobiologyinthecysticfibrosislungareviewwithselectedexperimentalobservations AT tzunglinou bicarbonateeffectsonantibacterialimmunityandmucusglycobiologyinthecysticfibrosislungareviewwithselectedexperimentalobservations AT samiradahesh bicarbonateeffectsonantibacterialimmunityandmucusglycobiologyinthecysticfibrosislungareviewwithselectedexperimentalobservations AT kathrynakong bicarbonateeffectsonantibacterialimmunityandmucusglycobiologyinthecysticfibrosislungareviewwithselectedexperimentalobservations AT victornizet bicarbonateeffectsonantibacterialimmunityandmucusglycobiologyinthecysticfibrosislungareviewwithselectedexperimentalobservations |