An open source extrusion bioprinter based on the E3D motion system and tool changer to enable FRESH and multimaterial bioprinting
Abstract Bioprinting is increasingly used to create complex tissue constructs for an array of research applications, and there are also increasing efforts to print tissues for transplantation. Bioprinting may also prove valuable in the context of drug screening for personalized medicine for treatmen...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2021-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-00931-1 |
| _version_ | 1818724295815200768 |
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| author | Adam Engberg Christina Stelzl Olle Eriksson Paul O’Callaghan Johan Kreuger |
| author_facet | Adam Engberg Christina Stelzl Olle Eriksson Paul O’Callaghan Johan Kreuger |
| author_sort | Adam Engberg |
| collection | DOAJ |
| description | Abstract Bioprinting is increasingly used to create complex tissue constructs for an array of research applications, and there are also increasing efforts to print tissues for transplantation. Bioprinting may also prove valuable in the context of drug screening for personalized medicine for treatment of diseases such as cancer. However, the rapidly expanding bioprinting research field is currently limited by access to bioprinters. To increase the availability of bioprinting technologies we present here an open source extrusion bioprinter based on the E3D motion system and tool changer to enable high-resolution multimaterial bioprinting. As proof of concept, the bioprinter is used to create collagen constructs using freeform reversible embedding of suspended hydrogels (FRESH) methodology, as well as multimaterial constructs composed of distinct sections of laminin and collagen. Data is presented demonstrating that the bioprinted constructs support growth of cells either seeded onto printed constructs or included in the bioink prior to bioprinting. This open source bioprinter is easily adapted for different bioprinting applications, and additional tools can be incorporated to increase the capabilities of the system. |
| first_indexed | 2024-12-17T21:24:09Z |
| format | Article |
| id | doaj.art-ea7f6922969646c992d59c4e42d5dff0 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-17T21:24:09Z |
| publishDate | 2021-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-ea7f6922969646c992d59c4e42d5dff02022-12-21T21:32:05ZengNature PortfolioScientific Reports2045-23222021-11-0111111110.1038/s41598-021-00931-1An open source extrusion bioprinter based on the E3D motion system and tool changer to enable FRESH and multimaterial bioprintingAdam Engberg0Christina Stelzl1Olle Eriksson2Paul O’Callaghan3Johan Kreuger4Department of Medical Cell Biology, Uppsala UniversityDepartment of Medical Cell Biology, Uppsala UniversityDepartment of Medical Cell Biology, Uppsala UniversityDepartment of Medical Cell Biology, Uppsala UniversityDepartment of Medical Cell Biology, Uppsala UniversityAbstract Bioprinting is increasingly used to create complex tissue constructs for an array of research applications, and there are also increasing efforts to print tissues for transplantation. Bioprinting may also prove valuable in the context of drug screening for personalized medicine for treatment of diseases such as cancer. However, the rapidly expanding bioprinting research field is currently limited by access to bioprinters. To increase the availability of bioprinting technologies we present here an open source extrusion bioprinter based on the E3D motion system and tool changer to enable high-resolution multimaterial bioprinting. As proof of concept, the bioprinter is used to create collagen constructs using freeform reversible embedding of suspended hydrogels (FRESH) methodology, as well as multimaterial constructs composed of distinct sections of laminin and collagen. Data is presented demonstrating that the bioprinted constructs support growth of cells either seeded onto printed constructs or included in the bioink prior to bioprinting. This open source bioprinter is easily adapted for different bioprinting applications, and additional tools can be incorporated to increase the capabilities of the system.https://doi.org/10.1038/s41598-021-00931-1 |
| spellingShingle | Adam Engberg Christina Stelzl Olle Eriksson Paul O’Callaghan Johan Kreuger An open source extrusion bioprinter based on the E3D motion system and tool changer to enable FRESH and multimaterial bioprinting Scientific Reports |
| title | An open source extrusion bioprinter based on the E3D motion system and tool changer to enable FRESH and multimaterial bioprinting |
| title_full | An open source extrusion bioprinter based on the E3D motion system and tool changer to enable FRESH and multimaterial bioprinting |
| title_fullStr | An open source extrusion bioprinter based on the E3D motion system and tool changer to enable FRESH and multimaterial bioprinting |
| title_full_unstemmed | An open source extrusion bioprinter based on the E3D motion system and tool changer to enable FRESH and multimaterial bioprinting |
| title_short | An open source extrusion bioprinter based on the E3D motion system and tool changer to enable FRESH and multimaterial bioprinting |
| title_sort | open source extrusion bioprinter based on the e3d motion system and tool changer to enable fresh and multimaterial bioprinting |
| url | https://doi.org/10.1038/s41598-021-00931-1 |
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