Antimicrobial Activity of Sorghum Phenolic Extract on Bovine Foodborne and Mastitis-Causing Pathogens
Antimicrobial resistance in bacterial pathogens associated with bovine mastitis and human foodborne illnesses from contaminated food and water have an impact on animal and human health. Phenolic compounds have antimicrobial properties and some specialty sorghum grains are high in phenolic compounds,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-05-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/5/594 |
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| author | Sydney E. Schnur Raghavendra G. Amachawadi Giovanna Baca Sarah Sexton-Bowser Davina H. Rhodes Dmitriy Smolensky Thomas J. Herald Ramasamy Perumal Daniel U. Thomson Tiruvoor G. Nagaraja |
| author_facet | Sydney E. Schnur Raghavendra G. Amachawadi Giovanna Baca Sarah Sexton-Bowser Davina H. Rhodes Dmitriy Smolensky Thomas J. Herald Ramasamy Perumal Daniel U. Thomson Tiruvoor G. Nagaraja |
| author_sort | Sydney E. Schnur |
| collection | DOAJ |
| description | Antimicrobial resistance in bacterial pathogens associated with bovine mastitis and human foodborne illnesses from contaminated food and water have an impact on animal and human health. Phenolic compounds have antimicrobial properties and some specialty sorghum grains are high in phenolic compounds, and the grain extract may have the potential as a natural antimicrobial alternative. The study’s objective was to determine antimicrobial effects of sorghum phenolic extract on bacterial pathogens that cause bovine mastitis and human foodborne illnesses. Bacterial pathogens tested included <i>Escherichia coli</i>, <i>Salmonella</i> Typhimurium, <i>Campylobacter jejuni</i>, <i>Campylobacter coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i>, <i>Klebsiella oxytoca</i>, <i>Staphylococcus aureus</i>, and <i>Enterococcus faecalis</i>. Antibacterial activities of sorghum phenolic extracts were determined by agar-well diffusion assay. Sorghum phenolic extract was added to the wells in concentrations of 0, 100, 200, 500, 1000, or 4000 µg/mL. The control wells did not receive phenolic extract. Plates were incubated for 18–24 h, and the diameter of each zone of inhibition was measured. The results indicated that sorghum phenolic extract had inhibitory effects on <i>Staphylococcus aureus</i>, <i>Enterococcus faecalis</i>, <i>Campylobacter jejuni</i>, and <i>Campylobacter coli</i>. |
| first_indexed | 2024-03-10T11:21:30Z |
| format | Article |
| id | doaj.art-ea83177bd9b441f9951103ee37380cd3 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T11:21:30Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-ea83177bd9b441f9951103ee37380cd32023-11-21T20:01:50ZengMDPI AGAntibiotics2079-63822021-05-0110559410.3390/antibiotics10050594Antimicrobial Activity of Sorghum Phenolic Extract on Bovine Foodborne and Mastitis-Causing PathogensSydney E. Schnur0Raghavendra G. Amachawadi1Giovanna Baca2Sarah Sexton-Bowser3Davina H. Rhodes4Dmitriy Smolensky5Thomas J. Herald6Ramasamy Perumal7Daniel U. Thomson8Tiruvoor G. Nagaraja9Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS 66506, USADepartment of Clinical Sciences, Kansas State University, Manhattan, KS 66506, USADepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS 66506, USADepartment of Agronomy, Kansas State University, Manhattan, KS 66506, USADepartment of Horticulture & Landscape Architecture, Colorado State University, Fort Collins, CO 80521, USACenter for Grain and Animal Health Research, USDA, Manhattan, KS 66502, USACenter for Grain and Animal Health Research, USDA, Manhattan, KS 66502, USAAgriculture Research Center, Kansas State University, Hays, KS 67601, USADepartment of Animal Sciences, Iowa State University, Ames, IA 50011, USADepartment of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan, KS 66506, USAAntimicrobial resistance in bacterial pathogens associated with bovine mastitis and human foodborne illnesses from contaminated food and water have an impact on animal and human health. Phenolic compounds have antimicrobial properties and some specialty sorghum grains are high in phenolic compounds, and the grain extract may have the potential as a natural antimicrobial alternative. The study’s objective was to determine antimicrobial effects of sorghum phenolic extract on bacterial pathogens that cause bovine mastitis and human foodborne illnesses. Bacterial pathogens tested included <i>Escherichia coli</i>, <i>Salmonella</i> Typhimurium, <i>Campylobacter jejuni</i>, <i>Campylobacter coli</i>, <i>Pseudomonas aeruginosa</i>, <i>Klebsiella pneumoniae</i>, <i>Klebsiella oxytoca</i>, <i>Staphylococcus aureus</i>, and <i>Enterococcus faecalis</i>. Antibacterial activities of sorghum phenolic extracts were determined by agar-well diffusion assay. Sorghum phenolic extract was added to the wells in concentrations of 0, 100, 200, 500, 1000, or 4000 µg/mL. The control wells did not receive phenolic extract. Plates were incubated for 18–24 h, and the diameter of each zone of inhibition was measured. The results indicated that sorghum phenolic extract had inhibitory effects on <i>Staphylococcus aureus</i>, <i>Enterococcus faecalis</i>, <i>Campylobacter jejuni</i>, and <i>Campylobacter coli</i>.https://www.mdpi.com/2079-6382/10/5/594antibacterial activityfoodborne pathogensmastitis pathogenssorghum grain extract |
| spellingShingle | Sydney E. Schnur Raghavendra G. Amachawadi Giovanna Baca Sarah Sexton-Bowser Davina H. Rhodes Dmitriy Smolensky Thomas J. Herald Ramasamy Perumal Daniel U. Thomson Tiruvoor G. Nagaraja Antimicrobial Activity of Sorghum Phenolic Extract on Bovine Foodborne and Mastitis-Causing Pathogens Antibiotics antibacterial activity foodborne pathogens mastitis pathogens sorghum grain extract |
| title | Antimicrobial Activity of Sorghum Phenolic Extract on Bovine Foodborne and Mastitis-Causing Pathogens |
| title_full | Antimicrobial Activity of Sorghum Phenolic Extract on Bovine Foodborne and Mastitis-Causing Pathogens |
| title_fullStr | Antimicrobial Activity of Sorghum Phenolic Extract on Bovine Foodborne and Mastitis-Causing Pathogens |
| title_full_unstemmed | Antimicrobial Activity of Sorghum Phenolic Extract on Bovine Foodborne and Mastitis-Causing Pathogens |
| title_short | Antimicrobial Activity of Sorghum Phenolic Extract on Bovine Foodborne and Mastitis-Causing Pathogens |
| title_sort | antimicrobial activity of sorghum phenolic extract on bovine foodborne and mastitis causing pathogens |
| topic | antibacterial activity foodborne pathogens mastitis pathogens sorghum grain extract |
| url | https://www.mdpi.com/2079-6382/10/5/594 |
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