Combination of Transcriptomic, Proteomic, and Metabolomic Analysis Reveals the Ripening Mechanism of Banana Pulp
The banana is one of the most important fruits in the world. Bananas undergo a rapid ripening process after harvest, resulting in a short shelf. In this study, the mechanism underlying pulp ripening of harvested bananas was investigated using integrated transcriptomic, proteomic, and metabolomic ana...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-09-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/9/10/523 |
| _version_ | 1818019974159007744 |
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| author | Taotao Li Ze Yun Qixian Wu Hongxia Qu Xuewu Duan Yueming Jiang |
| author_facet | Taotao Li Ze Yun Qixian Wu Hongxia Qu Xuewu Duan Yueming Jiang |
| author_sort | Taotao Li |
| collection | DOAJ |
| description | The banana is one of the most important fruits in the world. Bananas undergo a rapid ripening process after harvest, resulting in a short shelf. In this study, the mechanism underlying pulp ripening of harvested bananas was investigated using integrated transcriptomic, proteomic, and metabolomic analysis. Ribonucleic acid sequencing (RNA-Seq) revealed that a great number of genes related to transcriptional regulation, signal transduction, cell wall modification, and secondary metabolism were up-regulated during pulp ripening. At the protein level, 84 proteins were differentially expressed during pulp ripening, most of which were associated with energy metabolism, oxidation-reduction, cell wall metabolism, and starch degradation. According to partial least squares discriminant analysis, 33 proteins were identified as potential markers for separating different ripening stages of the fruit. In addition to ethylene’s central role, auxin signal transduction might be involved in regulating pulp ripening. Moreover, secondary metabolism, energy metabolism, and the protein metabolic process also played an important role in pulp ripening. In all, this study provided a better understanding of pulp ripening of harvested bananas. |
| first_indexed | 2024-04-14T07:59:00Z |
| format | Article |
| id | doaj.art-ea843b5c69fa41cc8008c09a8eecd691 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-04-14T07:59:00Z |
| publishDate | 2019-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-ea843b5c69fa41cc8008c09a8eecd6912022-12-22T02:04:57ZengMDPI AGBiomolecules2218-273X2019-09-0191052310.3390/biom9100523biom9100523Combination of Transcriptomic, Proteomic, and Metabolomic Analysis Reveals the Ripening Mechanism of Banana PulpTaotao Li0Ze Yun1Qixian Wu2Hongxia Qu3Xuewu Duan4Yueming Jiang5Key Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture/Guangdong Provincial Key Laboratory of Applied Botany/Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaKey Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture/Guangdong Provincial Key Laboratory of Applied Botany/Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaKey Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture/Guangdong Provincial Key Laboratory of Applied Botany/Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaKey Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture/Guangdong Provincial Key Laboratory of Applied Botany/Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaKey Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture/Guangdong Provincial Key Laboratory of Applied Botany/Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaKey Laboratory of Post-Harvest Handling of Fruits, Ministry of Agriculture/Guangdong Provincial Key Laboratory of Applied Botany/Key Laboratory of Plant Resources Conservation and Sustainable Utilization, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, ChinaThe banana is one of the most important fruits in the world. Bananas undergo a rapid ripening process after harvest, resulting in a short shelf. In this study, the mechanism underlying pulp ripening of harvested bananas was investigated using integrated transcriptomic, proteomic, and metabolomic analysis. Ribonucleic acid sequencing (RNA-Seq) revealed that a great number of genes related to transcriptional regulation, signal transduction, cell wall modification, and secondary metabolism were up-regulated during pulp ripening. At the protein level, 84 proteins were differentially expressed during pulp ripening, most of which were associated with energy metabolism, oxidation-reduction, cell wall metabolism, and starch degradation. According to partial least squares discriminant analysis, 33 proteins were identified as potential markers for separating different ripening stages of the fruit. In addition to ethylene’s central role, auxin signal transduction might be involved in regulating pulp ripening. Moreover, secondary metabolism, energy metabolism, and the protein metabolic process also played an important role in pulp ripening. In all, this study provided a better understanding of pulp ripening of harvested bananas.https://www.mdpi.com/2218-273X/9/10/523fruitsofteningauxinproteomictranscriptomicsignal transduction |
| spellingShingle | Taotao Li Ze Yun Qixian Wu Hongxia Qu Xuewu Duan Yueming Jiang Combination of Transcriptomic, Proteomic, and Metabolomic Analysis Reveals the Ripening Mechanism of Banana Pulp Biomolecules fruit softening auxin proteomic transcriptomic signal transduction |
| title | Combination of Transcriptomic, Proteomic, and Metabolomic Analysis Reveals the Ripening Mechanism of Banana Pulp |
| title_full | Combination of Transcriptomic, Proteomic, and Metabolomic Analysis Reveals the Ripening Mechanism of Banana Pulp |
| title_fullStr | Combination of Transcriptomic, Proteomic, and Metabolomic Analysis Reveals the Ripening Mechanism of Banana Pulp |
| title_full_unstemmed | Combination of Transcriptomic, Proteomic, and Metabolomic Analysis Reveals the Ripening Mechanism of Banana Pulp |
| title_short | Combination of Transcriptomic, Proteomic, and Metabolomic Analysis Reveals the Ripening Mechanism of Banana Pulp |
| title_sort | combination of transcriptomic proteomic and metabolomic analysis reveals the ripening mechanism of banana pulp |
| topic | fruit softening auxin proteomic transcriptomic signal transduction |
| url | https://www.mdpi.com/2218-273X/9/10/523 |
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