The Degradation Interface of Magnesium Based Alloys in Direct Contact with Human Primary Osteoblast Cells.
Magnesium alloys have been identified as a new generation material of orthopaedic implants. In vitro setups mimicking physiological conditions are promising for material / degradation analysis prior to in vivo studies however the direct influence of cell on the degradation mechanism has never been i...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4915630?pdf=render |
| _version_ | 1811243417884164096 |
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| author | Nezha Ahmad Agha Regine Willumeit-Römer Daniel Laipple Bérengère Luthringer Frank Feyerabend |
| author_facet | Nezha Ahmad Agha Regine Willumeit-Römer Daniel Laipple Bérengère Luthringer Frank Feyerabend |
| author_sort | Nezha Ahmad Agha |
| collection | DOAJ |
| description | Magnesium alloys have been identified as a new generation material of orthopaedic implants. In vitro setups mimicking physiological conditions are promising for material / degradation analysis prior to in vivo studies however the direct influence of cell on the degradation mechanism has never been investigated. For the first time, the direct, active, influence of human primary osteoblasts on magnesium-based materials (pure magnesium, Mg-2Ag and Mg-10Gd alloys) is studied for up to 14 days. Several parameters such as composition of the degradation interface (directly beneath the cells) are analysed with a scanning electron microscope equipped with energy dispersive X-ray and focused ion beam. Furthermore, influence of the materials on cell metabolism is examined via different parameters like active mineralisation process. The results are highlighting the influences of the selected alloying element on the initial cells metabolic activity. |
| first_indexed | 2024-04-12T14:07:04Z |
| format | Article |
| id | doaj.art-ea844871bb784f8dbe03aead67bb62e4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T14:07:04Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ea844871bb784f8dbe03aead67bb62e42022-12-22T03:30:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01116e015787410.1371/journal.pone.0157874The Degradation Interface of Magnesium Based Alloys in Direct Contact with Human Primary Osteoblast Cells.Nezha Ahmad AghaRegine Willumeit-RömerDaniel LaippleBérengère LuthringerFrank FeyerabendMagnesium alloys have been identified as a new generation material of orthopaedic implants. In vitro setups mimicking physiological conditions are promising for material / degradation analysis prior to in vivo studies however the direct influence of cell on the degradation mechanism has never been investigated. For the first time, the direct, active, influence of human primary osteoblasts on magnesium-based materials (pure magnesium, Mg-2Ag and Mg-10Gd alloys) is studied for up to 14 days. Several parameters such as composition of the degradation interface (directly beneath the cells) are analysed with a scanning electron microscope equipped with energy dispersive X-ray and focused ion beam. Furthermore, influence of the materials on cell metabolism is examined via different parameters like active mineralisation process. The results are highlighting the influences of the selected alloying element on the initial cells metabolic activity.http://europepmc.org/articles/PMC4915630?pdf=render |
| spellingShingle | Nezha Ahmad Agha Regine Willumeit-Römer Daniel Laipple Bérengère Luthringer Frank Feyerabend The Degradation Interface of Magnesium Based Alloys in Direct Contact with Human Primary Osteoblast Cells. PLoS ONE |
| title | The Degradation Interface of Magnesium Based Alloys in Direct Contact with Human Primary Osteoblast Cells. |
| title_full | The Degradation Interface of Magnesium Based Alloys in Direct Contact with Human Primary Osteoblast Cells. |
| title_fullStr | The Degradation Interface of Magnesium Based Alloys in Direct Contact with Human Primary Osteoblast Cells. |
| title_full_unstemmed | The Degradation Interface of Magnesium Based Alloys in Direct Contact with Human Primary Osteoblast Cells. |
| title_short | The Degradation Interface of Magnesium Based Alloys in Direct Contact with Human Primary Osteoblast Cells. |
| title_sort | degradation interface of magnesium based alloys in direct contact with human primary osteoblast cells |
| url | http://europepmc.org/articles/PMC4915630?pdf=render |
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