<i>Rhodotorula toruloides</i> Single Cell Oil Production Using <i>Eucalyptus urograndis</i> Hemicellulose Hydrolysate as a Carbon Source
Microbial oil is a potential substitute for vegetable oils in the biodiesel industry. Efforts to obtain cheap carbon sources for the cultivation of lipid-producing microorganisms comprise an active research area. This work aimed to extract the hemicellulose fraction from <i>Eucalyptus uograndi...
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| Format: | Article |
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MDPI AG
2020-02-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/13/4/795 |
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| author | Helberth Júnnior Santos Lopes Nemailla Bonturi Everson Alves Miranda |
| author_facet | Helberth Júnnior Santos Lopes Nemailla Bonturi Everson Alves Miranda |
| author_sort | Helberth Júnnior Santos Lopes |
| collection | DOAJ |
| description | Microbial oil is a potential substitute for vegetable oils in the biodiesel industry. Efforts to obtain cheap carbon sources for the cultivation of lipid-producing microorganisms comprise an active research area. This work aimed to extract the hemicellulose fraction from <i>Eucalyptus uograndis</i> and to use its hydrolysate as a carbon source for <i>Rhodotorula toruloides</i> (an oleaginous yeast) cultivation for microbial oil production. Hemicellulose hydrothermal extractions were performed at different temperatures, times, and ratios of solid to liquid (S/L). Temperature and time showed a stronger effect on the solubilization of hemicellulose. Hemicellulose extraction at 155 °C, 195 min, and an S/L ratio of 1/2 resulted in a hydrolysate with a xylose content of 37.0 g/l. <i>R. toruloides</i> cultivation in this hydrolysate showed that initial pH had a strong influence on cell growth. At an initial pH of 6.2, cells grew to 6.0 g/l of biomass with a lipid content of 50%. Therefore, we believe that <i>E. urograndis</i> hemicellulose hydrolysate could be a potential substrate for <i>R. toruloides</i> for lipid production based on the biorefinery concept. |
| first_indexed | 2024-04-11T13:04:22Z |
| format | Article |
| id | doaj.art-ea8518cd4ac0496db08d0106bfea1fe7 |
| institution | Directory Open Access Journal |
| issn | 1996-1073 |
| language | English |
| last_indexed | 2024-04-11T13:04:22Z |
| publishDate | 2020-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj.art-ea8518cd4ac0496db08d0106bfea1fe72022-12-22T04:22:51ZengMDPI AGEnergies1996-10732020-02-0113479510.3390/en13040795en13040795<i>Rhodotorula toruloides</i> Single Cell Oil Production Using <i>Eucalyptus urograndis</i> Hemicellulose Hydrolysate as a Carbon SourceHelberth Júnnior Santos Lopes0Nemailla Bonturi1Everson Alves Miranda2Department of Materials and Bioprocess Engineering, School of Chemical Engineering, State University of Campinas, Campinas SP 13083-852, BrazilInstitute of Technology, University of Tartu, 50411 Tartu, EstoniaDepartment of Materials and Bioprocess Engineering, School of Chemical Engineering, State University of Campinas, Campinas SP 13083-852, BrazilMicrobial oil is a potential substitute for vegetable oils in the biodiesel industry. Efforts to obtain cheap carbon sources for the cultivation of lipid-producing microorganisms comprise an active research area. This work aimed to extract the hemicellulose fraction from <i>Eucalyptus uograndis</i> and to use its hydrolysate as a carbon source for <i>Rhodotorula toruloides</i> (an oleaginous yeast) cultivation for microbial oil production. Hemicellulose hydrothermal extractions were performed at different temperatures, times, and ratios of solid to liquid (S/L). Temperature and time showed a stronger effect on the solubilization of hemicellulose. Hemicellulose extraction at 155 °C, 195 min, and an S/L ratio of 1/2 resulted in a hydrolysate with a xylose content of 37.0 g/l. <i>R. toruloides</i> cultivation in this hydrolysate showed that initial pH had a strong influence on cell growth. At an initial pH of 6.2, cells grew to 6.0 g/l of biomass with a lipid content of 50%. Therefore, we believe that <i>E. urograndis</i> hemicellulose hydrolysate could be a potential substrate for <i>R. toruloides</i> for lipid production based on the biorefinery concept.https://www.mdpi.com/1996-1073/13/4/795<i>eucalyptus urograndis</i><i>rhodotorula toruloides</i>hemicellulosehydrolysateacetic acidsingle cell oil |
| spellingShingle | Helberth Júnnior Santos Lopes Nemailla Bonturi Everson Alves Miranda <i>Rhodotorula toruloides</i> Single Cell Oil Production Using <i>Eucalyptus urograndis</i> Hemicellulose Hydrolysate as a Carbon Source Energies <i>eucalyptus urograndis</i> <i>rhodotorula toruloides</i> hemicellulose hydrolysate acetic acid single cell oil |
| title | <i>Rhodotorula toruloides</i> Single Cell Oil Production Using <i>Eucalyptus urograndis</i> Hemicellulose Hydrolysate as a Carbon Source |
| title_full | <i>Rhodotorula toruloides</i> Single Cell Oil Production Using <i>Eucalyptus urograndis</i> Hemicellulose Hydrolysate as a Carbon Source |
| title_fullStr | <i>Rhodotorula toruloides</i> Single Cell Oil Production Using <i>Eucalyptus urograndis</i> Hemicellulose Hydrolysate as a Carbon Source |
| title_full_unstemmed | <i>Rhodotorula toruloides</i> Single Cell Oil Production Using <i>Eucalyptus urograndis</i> Hemicellulose Hydrolysate as a Carbon Source |
| title_short | <i>Rhodotorula toruloides</i> Single Cell Oil Production Using <i>Eucalyptus urograndis</i> Hemicellulose Hydrolysate as a Carbon Source |
| title_sort | i rhodotorula toruloides i single cell oil production using i eucalyptus urograndis i hemicellulose hydrolysate as a carbon source |
| topic | <i>eucalyptus urograndis</i> <i>rhodotorula toruloides</i> hemicellulose hydrolysate acetic acid single cell oil |
| url | https://www.mdpi.com/1996-1073/13/4/795 |
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