Type IV SCCmec found in decade old Brazilian MRSA isolates
Methicillin-resistant Staphylococcus aureus (MRSA) commonly causes infection in hospitalized patients. Since its appearance in the 1960s, the SCCmec has evolved throughout the years into 5 different types (I-V), each bearing a different set of genes. Infection with MRSA SCCmec types I, II or III is...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
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| Series: | Brazilian Journal of Infectious Diseases |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300010&lng=en&tlng=en |
| _version_ | 1818612998196953088 |
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| author | Cristina Reinert John Anthony McCulloch Shinya Watanabe Teruyo Ito Keiichi Hiramatsu Elsa Masae Mamizuka |
| author_facet | Cristina Reinert John Anthony McCulloch Shinya Watanabe Teruyo Ito Keiichi Hiramatsu Elsa Masae Mamizuka |
| author_sort | Cristina Reinert |
| collection | DOAJ |
| description | Methicillin-resistant Staphylococcus aureus (MRSA) commonly causes infection in hospitalized patients. Since its appearance in the 1960s, the SCCmec has evolved throughout the years into 5 different types (I-V), each bearing a different set of genes. Infection with MRSA SCCmec types I, II or III is almost exclusively restricted to hospitalised patients. However, recently, community acquired MRSA (CA-MRSA) infections have been reported with increasing frequency, usually caused by a type IV SCCmec MRSA in nosocomial settings. We studied the prevalence of SCCmec types in 50 nosocomial strains collected from 1995 to 1999. The SCCmec complex type and presence of Panton-Valentine leukocidin (PVL) were determined by PCR. Strains had been previously typed by PFGE and were now typed by MLST. We found that 3 of the isolates studied bore a type IVc SCCmec all having different PFGE and MLST profiles (ST3, ST5 and ST88). All strains bearing a type III SCCmec belonged to MLST ST239 (Brazilian/Iberian clone). Only the strain which presented the ST5 profile bore the pvl gene. The type IVc SCCmec strains presented relatively lower levels of resistance to oxacillin in comparison to the type III SCCmec strains. The pattern of dissemination of the type IV SCCmec remains to be elucidated. The finding of strains carrying a type IV SCCmec in the present study among strains isolated at least 7 years ago indicates that clones bearing a type IV SCCmec have been present in Brazil for quite some time, and must have gone by undetected. |
| first_indexed | 2024-12-16T15:55:07Z |
| format | Article |
| id | doaj.art-ea8a7bdb8a6e432482f36117a846e0d7 |
| institution | Directory Open Access Journal |
| issn | 1678-4391 |
| language | English |
| last_indexed | 2024-12-16T15:55:07Z |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Infectious Diseases |
| spelling | doaj.art-ea8a7bdb8a6e432482f36117a846e0d72022-12-21T22:25:36ZengElsevierBrazilian Journal of Infectious Diseases1678-439112321321610.1590/S1413-86702008000300010S1413-86702008000300010Type IV SCCmec found in decade old Brazilian MRSA isolatesCristina Reinert0John Anthony McCulloch1Shinya Watanabe2Teruyo Ito3Keiichi Hiramatsu4Elsa Masae Mamizuka5Universidade de São PauloUniversidade de São PauloJuntendo UniversityJuntendo UniversityJuntendo UniversityUniversidade de São PauloMethicillin-resistant Staphylococcus aureus (MRSA) commonly causes infection in hospitalized patients. Since its appearance in the 1960s, the SCCmec has evolved throughout the years into 5 different types (I-V), each bearing a different set of genes. Infection with MRSA SCCmec types I, II or III is almost exclusively restricted to hospitalised patients. However, recently, community acquired MRSA (CA-MRSA) infections have been reported with increasing frequency, usually caused by a type IV SCCmec MRSA in nosocomial settings. We studied the prevalence of SCCmec types in 50 nosocomial strains collected from 1995 to 1999. The SCCmec complex type and presence of Panton-Valentine leukocidin (PVL) were determined by PCR. Strains had been previously typed by PFGE and were now typed by MLST. We found that 3 of the isolates studied bore a type IVc SCCmec all having different PFGE and MLST profiles (ST3, ST5 and ST88). All strains bearing a type III SCCmec belonged to MLST ST239 (Brazilian/Iberian clone). Only the strain which presented the ST5 profile bore the pvl gene. The type IVc SCCmec strains presented relatively lower levels of resistance to oxacillin in comparison to the type III SCCmec strains. The pattern of dissemination of the type IV SCCmec remains to be elucidated. The finding of strains carrying a type IV SCCmec in the present study among strains isolated at least 7 years ago indicates that clones bearing a type IV SCCmec have been present in Brazil for quite some time, and must have gone by undetected.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300010&lng=en&tlng=enNosocomial infectionstype IV SCCmecStaphylococcus aureusmethicillin-resistantBrazil |
| spellingShingle | Cristina Reinert John Anthony McCulloch Shinya Watanabe Teruyo Ito Keiichi Hiramatsu Elsa Masae Mamizuka Type IV SCCmec found in decade old Brazilian MRSA isolates Brazilian Journal of Infectious Diseases Nosocomial infections type IV SCCmec Staphylococcus aureus methicillin-resistant Brazil |
| title | Type IV SCCmec found in decade old Brazilian MRSA isolates |
| title_full | Type IV SCCmec found in decade old Brazilian MRSA isolates |
| title_fullStr | Type IV SCCmec found in decade old Brazilian MRSA isolates |
| title_full_unstemmed | Type IV SCCmec found in decade old Brazilian MRSA isolates |
| title_short | Type IV SCCmec found in decade old Brazilian MRSA isolates |
| title_sort | type iv sccmec found in decade old brazilian mrsa isolates |
| topic | Nosocomial infections type IV SCCmec Staphylococcus aureus methicillin-resistant Brazil |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300010&lng=en&tlng=en |
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