The Activity of Antimicrobial Peptides in Pediatric Celiac Disease
BackgroundCeliac disease (CD) is an immune-mediated disorder of the gut in which innate and adaptive responses are involved. Antimicrobial peptides (AMPs) constitute an arsenal of innate immunity regulators of paramount importance in the gut. However, the role of AMPs in CD is unclear.AimsTo evaluat...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Pediatrics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fped.2022.873793/full |
| _version_ | 1818470063690219520 |
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| author | Altinoy T. Kamilova Gulnoza K. Azizova Zulkhumar E. Umarnazarova Dilrabo A. Abdullaeva Svetlana I. Geller |
| author_facet | Altinoy T. Kamilova Gulnoza K. Azizova Zulkhumar E. Umarnazarova Dilrabo A. Abdullaeva Svetlana I. Geller |
| author_sort | Altinoy T. Kamilova |
| collection | DOAJ |
| description | BackgroundCeliac disease (CD) is an immune-mediated disorder of the gut in which innate and adaptive responses are involved. Antimicrobial peptides (AMPs) constitute an arsenal of innate immunity regulators of paramount importance in the gut. However, the role of AMPs in CD is unclear.AimsTo evaluate the levels of fecal β-defensin-2, fecal calprotectin (FC), and antibodies against bactericidal/permeability-increasing protein (BPI) in the serum of children with active CD and to compare them with those of healthy controls (HCs).MethodsWe examined 76 children with recently diagnosed CD between the age of 2–10 years (average age: 6.1 ± 1.2 years) and 32 HC (average age: 6.2 ± 3.8 years) in this study. We evaluated the level of fecal β-defensin-2 and FC levels in coprofiltrates, and the level of anti-BPI antibodies in blood serum. Correlation relationships between the parameters were assessed according to Pearson correlation coefficient.ResultsFecal β-defensin-2 concentration was greater in the CD group than in HC group, amounting to 99.6 ± 15.5 ng/mL and 64.0 ± 2.4 ng/mL, respectively (p < 0.02). The level of FC in the CD children was 35.4 ± 8.1 μg/g, while that in the control group was 19.1 ± 1.1 μg/g, (p < 0.05), representing a slightly increase. The concentration of anti-BPI antibodies in the CD and HC groups was 35.9 ± 10.1 U/mL and 5.2 ± 3.2 U/mL, respectively (p < 0.002). There was a strong and direct correlation between fecal β-defensin-2 and FC (r = 0.69), as well as a direct but weak relationship between fecal β-defensin-2 and anti-BPI antibodies (r = 0.35).ConclusionsOur data reinforce that fecal β-defensin-2 and anti-BPI antibodies are greatly increased in patients with active CD. These biomarkers may be components of epithelial innate immunity in the intestine, with each having a distinct functional role in intestinal6 mucosal defense. |
| first_indexed | 2024-04-13T21:32:54Z |
| format | Article |
| id | doaj.art-ea8bb27318414064a46574cfcaa2e383 |
| institution | Directory Open Access Journal |
| issn | 2296-2360 |
| language | English |
| last_indexed | 2024-04-13T21:32:54Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pediatrics |
| spelling | doaj.art-ea8bb27318414064a46574cfcaa2e3832022-12-22T02:29:05ZengFrontiers Media S.A.Frontiers in Pediatrics2296-23602022-06-011010.3389/fped.2022.873793873793The Activity of Antimicrobial Peptides in Pediatric Celiac DiseaseAltinoy T. KamilovaGulnoza K. AzizovaZulkhumar E. UmarnazarovaDilrabo A. AbdullaevaSvetlana I. GellerBackgroundCeliac disease (CD) is an immune-mediated disorder of the gut in which innate and adaptive responses are involved. Antimicrobial peptides (AMPs) constitute an arsenal of innate immunity regulators of paramount importance in the gut. However, the role of AMPs in CD is unclear.AimsTo evaluate the levels of fecal β-defensin-2, fecal calprotectin (FC), and antibodies against bactericidal/permeability-increasing protein (BPI) in the serum of children with active CD and to compare them with those of healthy controls (HCs).MethodsWe examined 76 children with recently diagnosed CD between the age of 2–10 years (average age: 6.1 ± 1.2 years) and 32 HC (average age: 6.2 ± 3.8 years) in this study. We evaluated the level of fecal β-defensin-2 and FC levels in coprofiltrates, and the level of anti-BPI antibodies in blood serum. Correlation relationships between the parameters were assessed according to Pearson correlation coefficient.ResultsFecal β-defensin-2 concentration was greater in the CD group than in HC group, amounting to 99.6 ± 15.5 ng/mL and 64.0 ± 2.4 ng/mL, respectively (p < 0.02). The level of FC in the CD children was 35.4 ± 8.1 μg/g, while that in the control group was 19.1 ± 1.1 μg/g, (p < 0.05), representing a slightly increase. The concentration of anti-BPI antibodies in the CD and HC groups was 35.9 ± 10.1 U/mL and 5.2 ± 3.2 U/mL, respectively (p < 0.002). There was a strong and direct correlation between fecal β-defensin-2 and FC (r = 0.69), as well as a direct but weak relationship between fecal β-defensin-2 and anti-BPI antibodies (r = 0.35).ConclusionsOur data reinforce that fecal β-defensin-2 and anti-BPI antibodies are greatly increased in patients with active CD. These biomarkers may be components of epithelial innate immunity in the intestine, with each having a distinct functional role in intestinal6 mucosal defense.https://www.frontiersin.org/articles/10.3389/fped.2022.873793/fullceliac diseasechildrenantimicrobial peptidesβ-defensin 2fecal calprotectinbactericidal permeability increasing protein |
| spellingShingle | Altinoy T. Kamilova Gulnoza K. Azizova Zulkhumar E. Umarnazarova Dilrabo A. Abdullaeva Svetlana I. Geller The Activity of Antimicrobial Peptides in Pediatric Celiac Disease Frontiers in Pediatrics celiac disease children antimicrobial peptides β-defensin 2 fecal calprotectin bactericidal permeability increasing protein |
| title | The Activity of Antimicrobial Peptides in Pediatric Celiac Disease |
| title_full | The Activity of Antimicrobial Peptides in Pediatric Celiac Disease |
| title_fullStr | The Activity of Antimicrobial Peptides in Pediatric Celiac Disease |
| title_full_unstemmed | The Activity of Antimicrobial Peptides in Pediatric Celiac Disease |
| title_short | The Activity of Antimicrobial Peptides in Pediatric Celiac Disease |
| title_sort | activity of antimicrobial peptides in pediatric celiac disease |
| topic | celiac disease children antimicrobial peptides β-defensin 2 fecal calprotectin bactericidal permeability increasing protein |
| url | https://www.frontiersin.org/articles/10.3389/fped.2022.873793/full |
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