Alterations of the gut microbiome and metabolic profile in CVB3-induced mice acute viral myocarditis
Abstract Background Acute viral myocarditis (AVMC) is an inflammatory disease of the myocardium. Evidence indicates that dysbiosis of gut microbiome and related metabolites intimately associated with cardiovascular diseases through the gut-heart axis. Methods We built mouse models of AVMC, then appl...
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| Format: | Article |
| Language: | English |
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BMC
2023-05-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-02863-4 |
| _version_ | 1797823047925760000 |
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| author | Qing Kong Lili Chen Xiaochun Zeng Feiyu Lu Yanlan Huang Weifeng Wu |
| author_facet | Qing Kong Lili Chen Xiaochun Zeng Feiyu Lu Yanlan Huang Weifeng Wu |
| author_sort | Qing Kong |
| collection | DOAJ |
| description | Abstract Background Acute viral myocarditis (AVMC) is an inflammatory disease of the myocardium. Evidence indicates that dysbiosis of gut microbiome and related metabolites intimately associated with cardiovascular diseases through the gut-heart axis. Methods We built mouse models of AVMC, then applied 16 S rDNA gene sequencing and UPLC-MS/MS metabolomics to explore variations of gut microbiome and disturbances of cardiac metabolic profiles. Results Compared with Control group, analysis of gut microbiota showed lower diversity in AVMC, decreased relative abundance of genera mainly belonging to the phyla Bacteroidetes, and increased of phyla Proteobacteria. Metabolomics analysis showed disturbances of cardiac metabolomics, including 62 increased and 84 decreased metabolites, and mainly assigned to lipid, amino acid, carbohydrate and nucleotide metabolism. The steroid hormone biosynthesis, cortisol synthesis and secretion pathway were particularly enriched in AVMC. Among them, such as estrone 3-sulfate, desoxycortone positively correlated with disturbed gut microbiome. Conclusion In summary, both the structure of the gut microbiome community and the cardiac metabolome were significantly changed in AVMC. Our findings suggest that gut microbiome may participate in the development of AVMC, the mechanism may be related to its role in dysregulated metabolites such as steroid hormone biosynthesis. |
| first_indexed | 2024-03-13T10:17:18Z |
| format | Article |
| id | doaj.art-ea8fdb5fea22443f9fe562fee1e49a83 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-13T10:17:18Z |
| publishDate | 2023-05-01 |
| publisher | BMC |
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| series | BMC Microbiology |
| spelling | doaj.art-ea8fdb5fea22443f9fe562fee1e49a832023-05-21T11:10:12ZengBMCBMC Microbiology1471-21802023-05-0123111110.1186/s12866-023-02863-4Alterations of the gut microbiome and metabolic profile in CVB3-induced mice acute viral myocarditisQing Kong0Lili Chen1Xiaochun Zeng2Feiyu Lu3Yanlan Huang4Weifeng Wu5Department of Cardiology, the First Affiliated Hospital of Guangxi Medical UniversityDepartment of Cardiology, the First Affiliated Hospital of Guangxi Medical UniversityDepartment of Cardiology, the First Affiliated Hospital of Guangxi Medical UniversityDepartment of Cardiology, the First Affiliated Hospital of Guangxi Medical UniversityDepartment of Cardiology, the First Affiliated Hospital of Guangxi Medical UniversityDepartment of Cardiology, the First Affiliated Hospital of Guangxi Medical UniversityAbstract Background Acute viral myocarditis (AVMC) is an inflammatory disease of the myocardium. Evidence indicates that dysbiosis of gut microbiome and related metabolites intimately associated with cardiovascular diseases through the gut-heart axis. Methods We built mouse models of AVMC, then applied 16 S rDNA gene sequencing and UPLC-MS/MS metabolomics to explore variations of gut microbiome and disturbances of cardiac metabolic profiles. Results Compared with Control group, analysis of gut microbiota showed lower diversity in AVMC, decreased relative abundance of genera mainly belonging to the phyla Bacteroidetes, and increased of phyla Proteobacteria. Metabolomics analysis showed disturbances of cardiac metabolomics, including 62 increased and 84 decreased metabolites, and mainly assigned to lipid, amino acid, carbohydrate and nucleotide metabolism. The steroid hormone biosynthesis, cortisol synthesis and secretion pathway were particularly enriched in AVMC. Among them, such as estrone 3-sulfate, desoxycortone positively correlated with disturbed gut microbiome. Conclusion In summary, both the structure of the gut microbiome community and the cardiac metabolome were significantly changed in AVMC. Our findings suggest that gut microbiome may participate in the development of AVMC, the mechanism may be related to its role in dysregulated metabolites such as steroid hormone biosynthesis.https://doi.org/10.1186/s12866-023-02863-4Gut microbiotaMetabolomicsOmics integrationMetabolic pathwaysViral myocarditis |
| spellingShingle | Qing Kong Lili Chen Xiaochun Zeng Feiyu Lu Yanlan Huang Weifeng Wu Alterations of the gut microbiome and metabolic profile in CVB3-induced mice acute viral myocarditis BMC Microbiology Gut microbiota Metabolomics Omics integration Metabolic pathways Viral myocarditis |
| title | Alterations of the gut microbiome and metabolic profile in CVB3-induced mice acute viral myocarditis |
| title_full | Alterations of the gut microbiome and metabolic profile in CVB3-induced mice acute viral myocarditis |
| title_fullStr | Alterations of the gut microbiome and metabolic profile in CVB3-induced mice acute viral myocarditis |
| title_full_unstemmed | Alterations of the gut microbiome and metabolic profile in CVB3-induced mice acute viral myocarditis |
| title_short | Alterations of the gut microbiome and metabolic profile in CVB3-induced mice acute viral myocarditis |
| title_sort | alterations of the gut microbiome and metabolic profile in cvb3 induced mice acute viral myocarditis |
| topic | Gut microbiota Metabolomics Omics integration Metabolic pathways Viral myocarditis |
| url | https://doi.org/10.1186/s12866-023-02863-4 |
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