Association mapping and validation of QTLs for flour yield in the soft winter wheat variety Kitahonami.
The winter wheat variety Kitahonami shows a superior flour yield in comparison to other Japanese soft wheat varieties. To map the quantitative trait loci (QTL) associated with this trait, association mapping was performed using a panel of lines from Kitahonami's pedigree, along with leading Jap...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4215981?pdf=render |
| _version_ | 1818409280482574336 |
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| author | Goro Ishikawa Kazuhiro Nakamura Hiroyuki Ito Mika Saito Mikako Sato Hironobu Jinno Yasuhiro Yoshimura Tsutomu Nishimura Hidekazu Maejima Yasushi Uehara Fuminori Kobayashi Toshiki Nakamura |
| author_facet | Goro Ishikawa Kazuhiro Nakamura Hiroyuki Ito Mika Saito Mikako Sato Hironobu Jinno Yasuhiro Yoshimura Tsutomu Nishimura Hidekazu Maejima Yasushi Uehara Fuminori Kobayashi Toshiki Nakamura |
| author_sort | Goro Ishikawa |
| collection | DOAJ |
| description | The winter wheat variety Kitahonami shows a superior flour yield in comparison to other Japanese soft wheat varieties. To map the quantitative trait loci (QTL) associated with this trait, association mapping was performed using a panel of lines from Kitahonami's pedigree, along with leading Japanese varieties and advanced breeding lines. Using a mixed linear model corrected for kernel types and familial relatedness, 62 marker-trait associations for flour yield were identified and classified into 21 QTLs. In eighteen of these, Kitahonami alleles showed positive effects. Pedigree analysis demonstrated that a continuous pyramiding of QTLs had occurred throughout the breeding history of Kitahonami. Linkage analyses using three sets of doubled haploid populations from crosses in which Kitahonami was used as a parent were performed, leading to the validation of five of the eight QTLs tested. Among these, QTLs on chromosomes 3B and 7A showed highly significant and consistent effects across the three populations. This study shows that pedigree-based association mapping using breeding materials can be a useful method for QTL identification at the early stages of breeding programs. |
| first_indexed | 2024-12-14T09:57:07Z |
| format | Article |
| id | doaj.art-ea9189b76bdb48f78c16be8e446f8d06 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T09:57:07Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ea9189b76bdb48f78c16be8e446f8d062022-12-21T23:07:22ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01910e11133710.1371/journal.pone.0111337Association mapping and validation of QTLs for flour yield in the soft winter wheat variety Kitahonami.Goro IshikawaKazuhiro NakamuraHiroyuki ItoMika SaitoMikako SatoHironobu JinnoYasuhiro YoshimuraTsutomu NishimuraHidekazu MaejimaYasushi UeharaFuminori KobayashiToshiki NakamuraThe winter wheat variety Kitahonami shows a superior flour yield in comparison to other Japanese soft wheat varieties. To map the quantitative trait loci (QTL) associated with this trait, association mapping was performed using a panel of lines from Kitahonami's pedigree, along with leading Japanese varieties and advanced breeding lines. Using a mixed linear model corrected for kernel types and familial relatedness, 62 marker-trait associations for flour yield were identified and classified into 21 QTLs. In eighteen of these, Kitahonami alleles showed positive effects. Pedigree analysis demonstrated that a continuous pyramiding of QTLs had occurred throughout the breeding history of Kitahonami. Linkage analyses using three sets of doubled haploid populations from crosses in which Kitahonami was used as a parent were performed, leading to the validation of five of the eight QTLs tested. Among these, QTLs on chromosomes 3B and 7A showed highly significant and consistent effects across the three populations. This study shows that pedigree-based association mapping using breeding materials can be a useful method for QTL identification at the early stages of breeding programs.http://europepmc.org/articles/PMC4215981?pdf=render |
| spellingShingle | Goro Ishikawa Kazuhiro Nakamura Hiroyuki Ito Mika Saito Mikako Sato Hironobu Jinno Yasuhiro Yoshimura Tsutomu Nishimura Hidekazu Maejima Yasushi Uehara Fuminori Kobayashi Toshiki Nakamura Association mapping and validation of QTLs for flour yield in the soft winter wheat variety Kitahonami. PLoS ONE |
| title | Association mapping and validation of QTLs for flour yield in the soft winter wheat variety Kitahonami. |
| title_full | Association mapping and validation of QTLs for flour yield in the soft winter wheat variety Kitahonami. |
| title_fullStr | Association mapping and validation of QTLs for flour yield in the soft winter wheat variety Kitahonami. |
| title_full_unstemmed | Association mapping and validation of QTLs for flour yield in the soft winter wheat variety Kitahonami. |
| title_short | Association mapping and validation of QTLs for flour yield in the soft winter wheat variety Kitahonami. |
| title_sort | association mapping and validation of qtls for flour yield in the soft winter wheat variety kitahonami |
| url | http://europepmc.org/articles/PMC4215981?pdf=render |
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