Protective functions of salvianolic acid B in PC-12 cells against hydrogen peroxide-triggered damage by mediation of microRNA-26a
Spinal cord injury (SCI) can lead to varying degrees of sensory and motor dysfunction. Salvianolic acid B (Sal-B) is the dominating bioactive constituent of Danshen, which has been reported to alleviate liver fibrosis and exert neuroprotective effects. But, the influence of Sal-B in SCI remains myst...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1673766 |
| _version_ | 1811291905073348608 |
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| author | Niansheng Liu Mingfu Fan |
| author_facet | Niansheng Liu Mingfu Fan |
| author_sort | Niansheng Liu |
| collection | DOAJ |
| description | Spinal cord injury (SCI) can lead to varying degrees of sensory and motor dysfunction. Salvianolic acid B (Sal-B) is the dominating bioactive constituent of Danshen, which has been reported to alleviate liver fibrosis and exert neuroprotective effects. But, the influence of Sal-B in SCI remains mysterious. The research planned to delve the protective function of Sal-B in hydrogen peroxide (H2O2)-caused PC-12 cell injury. H2O2-caused PC-12 cells injury model was built, CCK-8, Transwell and flow cytometry experiments were enforced to assess cell proliferation, migration and apoptosis. The microRNA (miR)-26a plasmid and the matching control were transfected into PC-12 cells, subsequently, the influence of miR-26a inhibition in H2O2-corrupted PC-12 cells was evaluated. The cell growth-correlated factors and PI3K/AKT and MEK/ERK pathways were assayed through western blot assay. Results corroborated that Sal-B eased H2O2-evoked injury in PC-12 cells. Ascended miR-26a was monitored in Sal-B and H2O2-exposed cells. MiR-26a inhibition annulled the protective action of Sal-B in H2O2-corrupted cells. The protective function of Sal-B was enabled through activating PI3K/AKT and MEK/ERK pathways. These findings delineated that Sal-B protected PC-12 cells against H2O2-caused injury through ascending miR-26a via initiating PI3K/AKT and MEK/ERK pathways.HighlightsH2O2 causes PC-12 cell injury;Sal-B eases H2O2-caused PC-12 cell injury;Sal-B protects PC-12 cells against H2O2-caused injury via elevating miR-26a;Sal-B activates AKT and MEK/ERK pathways via modulating miR-26a. |
| first_indexed | 2024-04-13T04:36:24Z |
| format | Article |
| id | doaj.art-ea956c48a62943feb4a2010f7d60cfe2 |
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| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-04-13T04:36:24Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
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| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-ea956c48a62943feb4a2010f7d60cfe22022-12-22T03:02:09ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014714030403710.1080/21691401.2019.1673766Protective functions of salvianolic acid B in PC-12 cells against hydrogen peroxide-triggered damage by mediation of microRNA-26aNiansheng Liu0Mingfu Fan1Department of Traumatology, North Medical District of Linyi People’s Hospital, Linyi, PR ChinaDepartment of Spinal Surgery, North Medical District of Linyi People’s Hospital, Linyi, PR ChinaSpinal cord injury (SCI) can lead to varying degrees of sensory and motor dysfunction. Salvianolic acid B (Sal-B) is the dominating bioactive constituent of Danshen, which has been reported to alleviate liver fibrosis and exert neuroprotective effects. But, the influence of Sal-B in SCI remains mysterious. The research planned to delve the protective function of Sal-B in hydrogen peroxide (H2O2)-caused PC-12 cell injury. H2O2-caused PC-12 cells injury model was built, CCK-8, Transwell and flow cytometry experiments were enforced to assess cell proliferation, migration and apoptosis. The microRNA (miR)-26a plasmid and the matching control were transfected into PC-12 cells, subsequently, the influence of miR-26a inhibition in H2O2-corrupted PC-12 cells was evaluated. The cell growth-correlated factors and PI3K/AKT and MEK/ERK pathways were assayed through western blot assay. Results corroborated that Sal-B eased H2O2-evoked injury in PC-12 cells. Ascended miR-26a was monitored in Sal-B and H2O2-exposed cells. MiR-26a inhibition annulled the protective action of Sal-B in H2O2-corrupted cells. The protective function of Sal-B was enabled through activating PI3K/AKT and MEK/ERK pathways. These findings delineated that Sal-B protected PC-12 cells against H2O2-caused injury through ascending miR-26a via initiating PI3K/AKT and MEK/ERK pathways.HighlightsH2O2 causes PC-12 cell injury;Sal-B eases H2O2-caused PC-12 cell injury;Sal-B protects PC-12 cells against H2O2-caused injury via elevating miR-26a;Sal-B activates AKT and MEK/ERK pathways via modulating miR-26a.https://www.tandfonline.com/doi/10.1080/21691401.2019.1673766PC-12 cellssalvianolic acid BH2O2microRNA-26aPI3K/AKTMEK/ERK |
| spellingShingle | Niansheng Liu Mingfu Fan Protective functions of salvianolic acid B in PC-12 cells against hydrogen peroxide-triggered damage by mediation of microRNA-26a Artificial Cells, Nanomedicine, and Biotechnology PC-12 cells salvianolic acid B H2O2 microRNA-26a PI3K/AKT MEK/ERK |
| title | Protective functions of salvianolic acid B in PC-12 cells against hydrogen peroxide-triggered damage by mediation of microRNA-26a |
| title_full | Protective functions of salvianolic acid B in PC-12 cells against hydrogen peroxide-triggered damage by mediation of microRNA-26a |
| title_fullStr | Protective functions of salvianolic acid B in PC-12 cells against hydrogen peroxide-triggered damage by mediation of microRNA-26a |
| title_full_unstemmed | Protective functions of salvianolic acid B in PC-12 cells against hydrogen peroxide-triggered damage by mediation of microRNA-26a |
| title_short | Protective functions of salvianolic acid B in PC-12 cells against hydrogen peroxide-triggered damage by mediation of microRNA-26a |
| title_sort | protective functions of salvianolic acid b in pc 12 cells against hydrogen peroxide triggered damage by mediation of microrna 26a |
| topic | PC-12 cells salvianolic acid B H2O2 microRNA-26a PI3K/AKT MEK/ERK |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1673766 |
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