Characterization of Andean Blueberry in Bioactive Compounds, Evaluation of Biological Properties, and In Vitro Bioaccessibility
The aim of this study was to evaluate Andean blueberries (<i>Vaccinium floribundum</i> Kunth) from Ecuador as a potential functional ingredient for the food and pharmaceutical industries. The analysis of bioactive compounds by HPLC–DAD–MS<sup>n</sup> determined a high content...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-10-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/10/1483 |
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| author | Nieves Baenas Jenny Ruales Diego A. Moreno Daniel Alejandro Barrio Carla M. Stinco Gabriela Martínez-Cifuentes Antonio J. Meléndez-Martínez Almudena García-Ruiz |
| author_facet | Nieves Baenas Jenny Ruales Diego A. Moreno Daniel Alejandro Barrio Carla M. Stinco Gabriela Martínez-Cifuentes Antonio J. Meléndez-Martínez Almudena García-Ruiz |
| author_sort | Nieves Baenas |
| collection | DOAJ |
| description | The aim of this study was to evaluate Andean blueberries (<i>Vaccinium floribundum</i> Kunth) from Ecuador as a potential functional ingredient for the food and pharmaceutical industries. The analysis of bioactive compounds by HPLC–DAD–MS<sup>n</sup> determined a high content of (poly)phenols, mainly anthocyanins, and the presence of the carotenoid lutein. Regarding its biological properties, Andean blueberry did not show toxicity by the zebrafish embryogenesis test, showing also a lack of the antinutrients lectins. Moreover, the results of in vitro and in vivo antioxidant capacity evaluation suggested its possibility to be used as natural antioxidant. This fruit also exhibited antimicrobial activity toward <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> in low doses. Finally, in vitro gastrointestinal (GI) digestion showed a partial bioaccessibility of (poly) phenols (~50% at the final step), showing high antioxidant capacity in the different GI phases. These results revealed Andean blueberry as an interesting candidate for being used as a functional ingredient and the development of further in vivo and clinical assays. |
| first_indexed | 2024-03-10T15:32:59Z |
| format | Article |
| id | doaj.art-ea958323965b49e797543b2aa8b5ab64 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T15:32:59Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-ea958323965b49e797543b2aa8b5ab642023-11-20T17:28:24ZengMDPI AGFoods2304-81582020-10-01910148310.3390/foods9101483Characterization of Andean Blueberry in Bioactive Compounds, Evaluation of Biological Properties, and In Vitro BioaccessibilityNieves Baenas0Jenny Ruales1Diego A. Moreno2Daniel Alejandro Barrio3Carla M. Stinco4Gabriela Martínez-Cifuentes5Antonio J. Meléndez-Martínez6Almudena García-Ruiz7Department of Food Technology, Food Science and Nutrition, Faculty of Veterinary Sciences, Regional Campus of International Excellence “Campus Mare-Nostrum”, University of Murcia, Campus de Espinardo, 30100 Murcia, SpainDepartment of Food Science and Biotechnology, Escuela Politécnica National, Quito 17-01-2759, EcuadorPhytochemistry and Healthy Foods Lab., Department of Food Science and Technology, CEBAS-CSIC, Campus de Espinardo-Edificio 25, E-30100 Murcia, SpainUniversidad Nacional de Río Negro, CIT Río Negro, Don Bosco y Leloir s/n, 8500 Río Negro, 8500 Viedma, ArgentinaFood Colour & Quality Lab., Department of Nutrition & Food Science, Universidad de Sevilla, Facultad de Farmacia, 41012 Sevilla, SpainDepartment of Food Science and Biotechnology, Escuela Politécnica National, Quito 17-01-2759, EcuadorFood Colour & Quality Lab., Department of Nutrition & Food Science, Universidad de Sevilla, Facultad de Farmacia, 41012 Sevilla, SpainDepartment of Food Science and Biotechnology, Escuela Politécnica National, Quito 17-01-2759, EcuadorThe aim of this study was to evaluate Andean blueberries (<i>Vaccinium floribundum</i> Kunth) from Ecuador as a potential functional ingredient for the food and pharmaceutical industries. The analysis of bioactive compounds by HPLC–DAD–MS<sup>n</sup> determined a high content of (poly)phenols, mainly anthocyanins, and the presence of the carotenoid lutein. Regarding its biological properties, Andean blueberry did not show toxicity by the zebrafish embryogenesis test, showing also a lack of the antinutrients lectins. Moreover, the results of in vitro and in vivo antioxidant capacity evaluation suggested its possibility to be used as natural antioxidant. This fruit also exhibited antimicrobial activity toward <i>Staphylococcus aureus</i> and <i>Escherichia coli</i> in low doses. Finally, in vitro gastrointestinal (GI) digestion showed a partial bioaccessibility of (poly) phenols (~50% at the final step), showing high antioxidant capacity in the different GI phases. These results revealed Andean blueberry as an interesting candidate for being used as a functional ingredient and the development of further in vivo and clinical assays.https://www.mdpi.com/2304-8158/9/10/1483mortiño<i>Vaccinium floribundum</i>HPLC–MS/MSanthocyaninsantioxidantantimicrobial |
| spellingShingle | Nieves Baenas Jenny Ruales Diego A. Moreno Daniel Alejandro Barrio Carla M. Stinco Gabriela Martínez-Cifuentes Antonio J. Meléndez-Martínez Almudena García-Ruiz Characterization of Andean Blueberry in Bioactive Compounds, Evaluation of Biological Properties, and In Vitro Bioaccessibility Foods mortiño <i>Vaccinium floribundum</i> HPLC–MS/MS anthocyanins antioxidant antimicrobial |
| title | Characterization of Andean Blueberry in Bioactive Compounds, Evaluation of Biological Properties, and In Vitro Bioaccessibility |
| title_full | Characterization of Andean Blueberry in Bioactive Compounds, Evaluation of Biological Properties, and In Vitro Bioaccessibility |
| title_fullStr | Characterization of Andean Blueberry in Bioactive Compounds, Evaluation of Biological Properties, and In Vitro Bioaccessibility |
| title_full_unstemmed | Characterization of Andean Blueberry in Bioactive Compounds, Evaluation of Biological Properties, and In Vitro Bioaccessibility |
| title_short | Characterization of Andean Blueberry in Bioactive Compounds, Evaluation of Biological Properties, and In Vitro Bioaccessibility |
| title_sort | characterization of andean blueberry in bioactive compounds evaluation of biological properties and in vitro bioaccessibility |
| topic | mortiño <i>Vaccinium floribundum</i> HPLC–MS/MS anthocyanins antioxidant antimicrobial |
| url | https://www.mdpi.com/2304-8158/9/10/1483 |
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