Dynamic changes in the endophytic bacterial community during maturation of Amorphophallus muelleri seeds
The seed microbiota is considered to be the starting point of the accumulation of plant microbiota, which is conducive to the preservation and germination of seeds and the establishment and development of seedlings. Our understanding of the colonization and migration dynamics of microbial taxa durin...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.996854/full |
| _version_ | 1811204343852957696 |
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| author | Min Yang Min Yang Ying Qi Jiani Liu Zhixing Wu Penghua Gao Zebin Chen Feiyan Huang Lei Yu |
| author_facet | Min Yang Min Yang Ying Qi Jiani Liu Zhixing Wu Penghua Gao Zebin Chen Feiyan Huang Lei Yu |
| author_sort | Min Yang |
| collection | DOAJ |
| description | The seed microbiota is considered to be the starting point of the accumulation of plant microbiota, which is conducive to the preservation and germination of seeds and the establishment and development of seedlings. Our understanding of the colonization and migration dynamics of microbial taxa during seed development and maturation is still limited. This study used 16S rRNA high-throughput sequencing to investigate the dynamic changes in the composition and diversity of the endophytic bacterial community during maturation of Amorphophallus muelleri seeds. The results showed that as seeds matured (green to red), the Shannon index of their endophytic bacterial community first decreased and then increased, and the ACE and Chao1 indices of the endophytic bacterial community decreased gradually. The Shannon, ACE, and Chao1 indices of the endophytic bacterial community in the seed coat first decreased and then increased. Principal coordinate analysis of the bacterial communities revealed that the seed coat at different maturity stages showed significantly distinct bacterial communities and formed different clusters according to maturity stage. The bacterial communities of green and red seeds showed a clear separation, but they both overlapped with those of yellow seeds, indicating that some core taxa were present throughout seed maturation, but their relative abundance was dynamically changing. As the seeds grew more mature, the relative abundance of some bacterial communities with plant growth-promoting traits and others correlated with plant resistance (e.g., Burkholderia-Caballeronia-Paraburkholderia, Bacillus, Pseudomonas, Bradyrhizobium, Streptomyces) tended to increase and peaked in fully mature seeds and seed coats. The endophytic bacterial community of A. muelleri seeds seems to be driven by the seed maturation state, which can provide a theoretical basis for a comprehensive understanding of the assembly process of the microbial community during A. muelleri seed maturation. |
| first_indexed | 2024-04-12T03:11:33Z |
| format | Article |
| id | doaj.art-ea9706e404f54859ab647b72bd937b76 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T03:11:33Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ea9706e404f54859ab647b72bd937b762022-12-22T03:50:20ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.996854996854Dynamic changes in the endophytic bacterial community during maturation of Amorphophallus muelleri seedsMin Yang0Min Yang1Ying Qi2Jiani Liu3Zhixing Wu4Penghua Gao5Zebin Chen6Feiyan Huang7Lei Yu8Yunnan Urban Agricultural Engineering and Technological Research Center, College of Agronomy, Kunming University, Kunming, ChinaCollege of Plant Protection, Yunnan Agricultural University, Kunming, ChinaYunnan Urban Agricultural Engineering and Technological Research Center, College of Agronomy, Kunming University, Kunming, ChinaYunnan Urban Agricultural Engineering and Technological Research Center, College of Agronomy, Kunming University, Kunming, ChinaYunnan Urban Agricultural Engineering and Technological Research Center, College of Agronomy, Kunming University, Kunming, ChinaYunnan Urban Agricultural Engineering and Technological Research Center, College of Agronomy, Kunming University, Kunming, ChinaYunnan Urban Agricultural Engineering and Technological Research Center, College of Agronomy, Kunming University, Kunming, ChinaYunnan Urban Agricultural Engineering and Technological Research Center, College of Agronomy, Kunming University, Kunming, ChinaYunnan Urban Agricultural Engineering and Technological Research Center, College of Agronomy, Kunming University, Kunming, ChinaThe seed microbiota is considered to be the starting point of the accumulation of plant microbiota, which is conducive to the preservation and germination of seeds and the establishment and development of seedlings. Our understanding of the colonization and migration dynamics of microbial taxa during seed development and maturation is still limited. This study used 16S rRNA high-throughput sequencing to investigate the dynamic changes in the composition and diversity of the endophytic bacterial community during maturation of Amorphophallus muelleri seeds. The results showed that as seeds matured (green to red), the Shannon index of their endophytic bacterial community first decreased and then increased, and the ACE and Chao1 indices of the endophytic bacterial community decreased gradually. The Shannon, ACE, and Chao1 indices of the endophytic bacterial community in the seed coat first decreased and then increased. Principal coordinate analysis of the bacterial communities revealed that the seed coat at different maturity stages showed significantly distinct bacterial communities and formed different clusters according to maturity stage. The bacterial communities of green and red seeds showed a clear separation, but they both overlapped with those of yellow seeds, indicating that some core taxa were present throughout seed maturation, but their relative abundance was dynamically changing. As the seeds grew more mature, the relative abundance of some bacterial communities with plant growth-promoting traits and others correlated with plant resistance (e.g., Burkholderia-Caballeronia-Paraburkholderia, Bacillus, Pseudomonas, Bradyrhizobium, Streptomyces) tended to increase and peaked in fully mature seeds and seed coats. The endophytic bacterial community of A. muelleri seeds seems to be driven by the seed maturation state, which can provide a theoretical basis for a comprehensive understanding of the assembly process of the microbial community during A. muelleri seed maturation.https://www.frontiersin.org/articles/10.3389/fmicb.2022.996854/fullAmorphophallus muelleriseedsmaturationendophytic bacterial communitydynamic |
| spellingShingle | Min Yang Min Yang Ying Qi Jiani Liu Zhixing Wu Penghua Gao Zebin Chen Feiyan Huang Lei Yu Dynamic changes in the endophytic bacterial community during maturation of Amorphophallus muelleri seeds Frontiers in Microbiology Amorphophallus muelleri seeds maturation endophytic bacterial community dynamic |
| title | Dynamic changes in the endophytic bacterial community during maturation of Amorphophallus muelleri seeds |
| title_full | Dynamic changes in the endophytic bacterial community during maturation of Amorphophallus muelleri seeds |
| title_fullStr | Dynamic changes in the endophytic bacterial community during maturation of Amorphophallus muelleri seeds |
| title_full_unstemmed | Dynamic changes in the endophytic bacterial community during maturation of Amorphophallus muelleri seeds |
| title_short | Dynamic changes in the endophytic bacterial community during maturation of Amorphophallus muelleri seeds |
| title_sort | dynamic changes in the endophytic bacterial community during maturation of amorphophallus muelleri seeds |
| topic | Amorphophallus muelleri seeds maturation endophytic bacterial community dynamic |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.996854/full |
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