Effect of various decontamination procedures on disposable N95 mask integrity and SARS-CoV-2 infectivity
Abstract Introduction: The COVID-19 pandemic has created a high demand on personal protective equipment, including disposable N95 masks. Given the need for mask reuse, we tested the feasibility of vaporized hydrogen peroxide (VHP), ultraviolet light (UV), and ethanol decontamination strategies on...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Cambridge University Press
2021-01-01
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| Series: | Journal of Clinical and Translational Science |
| Subjects: | |
| Online Access: | https://www.cambridge.org/core/product/identifier/S205986612000494X/type/journal_article |
| _version_ | 1811156526012825600 |
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| author | Jeffrey S. Smith Haley Hanseler John Welle Rogan Rattray Mary Campbell Tacy Brotherton Tarsem Moudgil Thomas F. Pack Keith Wegmann Shawn Jensen Justin Jin Carlo B. Bifulco Scott A. Prahl Bernard A. Fox Nicholas L. Stucky |
| author_facet | Jeffrey S. Smith Haley Hanseler John Welle Rogan Rattray Mary Campbell Tacy Brotherton Tarsem Moudgil Thomas F. Pack Keith Wegmann Shawn Jensen Justin Jin Carlo B. Bifulco Scott A. Prahl Bernard A. Fox Nicholas L. Stucky |
| author_sort | Jeffrey S. Smith |
| collection | DOAJ |
| description |
Abstract
Introduction:
The COVID-19 pandemic has created a high demand on personal protective equipment, including disposable N95 masks. Given the need for mask reuse, we tested the feasibility of vaporized hydrogen peroxide (VHP), ultraviolet light (UV), and ethanol decontamination strategies on N95 mask integrity and the ability to remove the infectious potential of SARS-CoV-2.
Methods:
Disposable N95 masks, including medical grade (1860, 1870+) and industrial grade (8511) masks, were treated by VHP, UV, and ethanol decontamination. Mask degradation was tested using a quantitative respirator fit testing. Pooled clinical samples of SARS-CoV-2 were applied to mask samples, treated, and then either sent immediately for real-time reverse transcriptase–polymerase chain reaction (RT-PCR) or incubated with Vero E6 cells to assess for virucidal effect.
Results:
Both ethanol and UV decontamination showed functional degradation to different degrees while VHP treatment showed no significant change after two treatments. We also report a single SARS-CoV-2 virucidal experiment using Vero E6 cell infection in which only ethanol treatment eliminated detectable SARS-CoV-2 RNA.
Conclusions:
We hope our data will guide further research for evidenced-based decisions for disposable N95 mask reuse and help protect caregivers from SARS-CoV-2 and other pathogens.
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| first_indexed | 2024-04-10T04:53:10Z |
| format | Article |
| id | doaj.art-ea972a4eaf344f10a35f931c8abb5ab5 |
| institution | Directory Open Access Journal |
| issn | 2059-8661 |
| language | English |
| last_indexed | 2024-04-10T04:53:10Z |
| publishDate | 2021-01-01 |
| publisher | Cambridge University Press |
| record_format | Article |
| series | Journal of Clinical and Translational Science |
| spelling | doaj.art-ea972a4eaf344f10a35f931c8abb5ab52023-03-09T12:31:04ZengCambridge University PressJournal of Clinical and Translational Science2059-86612021-01-01510.1017/cts.2020.494Effect of various decontamination procedures on disposable N95 mask integrity and SARS-CoV-2 infectivityJeffrey S. Smith0Haley Hanseler1John Welle2Rogan Rattray3Mary Campbell4Tacy Brotherton5Tarsem Moudgil6Thomas F. Pack7https://orcid.org/0000-0002-1240-8324Keith Wegmann8Shawn Jensen9Justin Jin10Carlo B. Bifulco11Scott A. Prahl12Bernard A. Fox13Nicholas L. Stucky14https://orcid.org/0000-0002-6610-5353Providence Portland Medical Center, Department of Medicine, Portland, Oregon, USAOHSU Medical School, Portland, Oregon, USARegional Pathology, Providence St. Joseph Health, Portland, Oregon, USARegional Pathology, Providence St. Joseph Health, Portland, Oregon, USARegional Pathology, Providence St. Joseph Health, Portland, Oregon, USACancer Research Animal Division, Robert W. Franz Cancer Center, Earle A. Chiles Research Institute, Providence Cancer Institute, Portland, Oregon, USAMolecular & Tumor Immunology, Robert W. Franz Cancer Center, Earle A. Chiles Research Institute, Providence Cancer Institute, Portland, Oregon, USAAxovant Gene Therapies, Ltd, Durham, North Carolina, USAMolecular & Tumor Immunology, Robert W. Franz Cancer Center, Earle A. Chiles Research Institute, Providence Cancer Institute, Portland, Oregon, USAMolecular & Tumor Immunology, Robert W. Franz Cancer Center, Earle A. Chiles Research Institute, Providence Cancer Institute, Portland, Oregon, USAProvidence Portland Medical Center, Department of Medicine, Portland, Oregon, USARegional Pathology, Providence St. Joseph Health, Portland, Oregon, USA Translational Molecular Pathology and Molecular Genomics, Robert W. Franz Cancer Center, Earle A. Chiles Research Institute, Providence Cancer Institute, Portland, Oregon, USAElectrical Engineering and Renewable Energy, Oregon Institute of Technology, Wilsonville, Oregon, USAMolecular & Tumor Immunology, Robert W. Franz Cancer Center, Earle A. Chiles Research Institute, Providence Cancer Institute, Portland, Oregon, USA Department of Molecular Microbiology and Immunology, and Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon, USAProvidence Portland Medical Center, Department of Medicine, Portland, Oregon, USA Abstract Introduction: The COVID-19 pandemic has created a high demand on personal protective equipment, including disposable N95 masks. Given the need for mask reuse, we tested the feasibility of vaporized hydrogen peroxide (VHP), ultraviolet light (UV), and ethanol decontamination strategies on N95 mask integrity and the ability to remove the infectious potential of SARS-CoV-2. Methods: Disposable N95 masks, including medical grade (1860, 1870+) and industrial grade (8511) masks, were treated by VHP, UV, and ethanol decontamination. Mask degradation was tested using a quantitative respirator fit testing. Pooled clinical samples of SARS-CoV-2 were applied to mask samples, treated, and then either sent immediately for real-time reverse transcriptase–polymerase chain reaction (RT-PCR) or incubated with Vero E6 cells to assess for virucidal effect. Results: Both ethanol and UV decontamination showed functional degradation to different degrees while VHP treatment showed no significant change after two treatments. We also report a single SARS-CoV-2 virucidal experiment using Vero E6 cell infection in which only ethanol treatment eliminated detectable SARS-CoV-2 RNA. Conclusions: We hope our data will guide further research for evidenced-based decisions for disposable N95 mask reuse and help protect caregivers from SARS-CoV-2 and other pathogens. https://www.cambridge.org/core/product/identifier/S205986612000494X/type/journal_articleInfectious diseasesmask decontaminationSARS-CoV-2COVID-19vaporized hydrogen peroxideultraviolet germicidal irradiationN95 |
| spellingShingle | Jeffrey S. Smith Haley Hanseler John Welle Rogan Rattray Mary Campbell Tacy Brotherton Tarsem Moudgil Thomas F. Pack Keith Wegmann Shawn Jensen Justin Jin Carlo B. Bifulco Scott A. Prahl Bernard A. Fox Nicholas L. Stucky Effect of various decontamination procedures on disposable N95 mask integrity and SARS-CoV-2 infectivity Journal of Clinical and Translational Science Infectious diseases mask decontamination SARS-CoV-2 COVID-19 vaporized hydrogen peroxide ultraviolet germicidal irradiation N95 |
| title | Effect of various decontamination procedures on disposable N95 mask integrity and SARS-CoV-2 infectivity |
| title_full | Effect of various decontamination procedures on disposable N95 mask integrity and SARS-CoV-2 infectivity |
| title_fullStr | Effect of various decontamination procedures on disposable N95 mask integrity and SARS-CoV-2 infectivity |
| title_full_unstemmed | Effect of various decontamination procedures on disposable N95 mask integrity and SARS-CoV-2 infectivity |
| title_short | Effect of various decontamination procedures on disposable N95 mask integrity and SARS-CoV-2 infectivity |
| title_sort | effect of various decontamination procedures on disposable n95 mask integrity and sars cov 2 infectivity |
| topic | Infectious diseases mask decontamination SARS-CoV-2 COVID-19 vaporized hydrogen peroxide ultraviolet germicidal irradiation N95 |
| url | https://www.cambridge.org/core/product/identifier/S205986612000494X/type/journal_article |
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