Melatonin increases AKT and SOD gene and protein expressions in diabetic rats
Diabetes mellitus (DM) is a chronic metabolic disease marked by hyperglycemia due to insulin deficiency or insulin resistance leading to many chronic complications. It is thus important to manage diabetes effectively in order to prevent and or delay these complications. Melatonin is produced by the...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-04-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S240584402404670X |
| _version_ | 1827572225936457728 |
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| author | Mohamed Lotfy Aalaa Khattab Mohammed Shata Ahmad Alhasbani Abdallah Khalaf Saeed Alsaeedi Mahdi Thaker Hazza Said Harun Tumi Hassan Alzahmi Omar Alblooshi Mohamad Hamdan Amjad Hussein Biduth Kundu Ernest A. Adeghate |
| author_facet | Mohamed Lotfy Aalaa Khattab Mohammed Shata Ahmad Alhasbani Abdallah Khalaf Saeed Alsaeedi Mahdi Thaker Hazza Said Harun Tumi Hassan Alzahmi Omar Alblooshi Mohamad Hamdan Amjad Hussein Biduth Kundu Ernest A. Adeghate |
| author_sort | Mohamed Lotfy |
| collection | DOAJ |
| description | Diabetes mellitus (DM) is a chronic metabolic disease marked by hyperglycemia due to insulin deficiency or insulin resistance leading to many chronic complications. It is thus important to manage diabetes effectively in order to prevent and or delay these complications. Melatonin is produced by the pineal gland and regulates the wake-sleep circadian rhythm. Existing evidence suggests that melatonin may be effective in the management of DM. However, the evidence on the mechanism of the beneficial effect melatonin as a treatment for DM is limited. In this study, we investigated the effect of melatonin treatment on blood glucose, insulin (INS), AKT and superoxide dismutase (SOD) gene levels in diabetic rats. Non-diabetic and diabetic rats were treated orally for 4 weeks with either 25 mg or 50 mg/kg body weight of melatonin. At the end of the study, pancreatic and liver tissues morphology, glucose homeostasis, serum insulin and SOD levels, hepatic gene and protein expression of SOD as protecting antioxidant enzyme and AKT as central element involved in PI3K/AKT insulin signaling pathway were estimated. Melatonin treated diabetic rats showed reduced hyperglycemia, and increased serum insulin and SOD levels. In addition, melatonin induced an increased gene and protein expression of SOD and AKT. In conclusion, melatonin may play a role in treating diabetic rats via stimulation of insulin secretion, insulin signaling and reduction in oxidative stress. |
| first_indexed | 2024-04-24T16:26:20Z |
| format | Article |
| id | doaj.art-ea9caedaf41644c5b82fdd916ed0f9c6 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T16:26:20Z |
| publishDate | 2024-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-ea9caedaf41644c5b82fdd916ed0f9c62024-03-31T04:37:37ZengElsevierHeliyon2405-84402024-04-01107e28639Melatonin increases AKT and SOD gene and protein expressions in diabetic ratsMohamed Lotfy0Aalaa Khattab1Mohammed Shata2Ahmad Alhasbani3Abdallah Khalaf4Saeed Alsaeedi5Mahdi Thaker6Hazza Said7Harun Tumi8Hassan Alzahmi9Omar Alblooshi10Mohamad Hamdan11Amjad Hussein12Biduth Kundu13Ernest A. Adeghate14Biology Department, College of Science, United Arab Emirates University, Al Ain, United Arab Emirates; Corresponding author. Biology Department, College of Science, United Arab Emirates University, Al Ain, United Arab Emirates.Faculty of Dentistry, The British University in Egypt, El Sherouk City, Cairo, EgyptBiology Department, College of Science, United Arab Emirates University, Al Ain, United Arab EmiratesBiology Department, College of Science, United Arab Emirates University, Al Ain, United Arab EmiratesBiology Department, College of Science, United Arab Emirates University, Al Ain, United Arab EmiratesBiology Department, College of Science, United Arab Emirates University, Al Ain, United Arab EmiratesBiology Department, College of Science, United Arab Emirates University, Al Ain, United Arab EmiratesBiology Department, College of Science, United Arab Emirates University, Al Ain, United Arab EmiratesBiology Department, College of Science, United Arab Emirates University, Al Ain, United Arab EmiratesBiology Department, College of Science, United Arab Emirates University, Al Ain, United Arab EmiratesBiology Department, College of Science, United Arab Emirates University, Al Ain, United Arab EmiratesBiology Department, College of Science, United Arab Emirates University, Al Ain, United Arab EmiratesBiology Department, College of Science, United Arab Emirates University, Al Ain, United Arab EmiratesBiology Department, College of Science, United Arab Emirates University, Al Ain, United Arab EmiratesDepartment of Anatomy, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates; Zayed Centre for Health Sciences, College of Science, United Arab Emirates University, Al Ain, United Arab Emirates; Corresponding author. Department of Anatomy, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain, United Arab Emirates.Diabetes mellitus (DM) is a chronic metabolic disease marked by hyperglycemia due to insulin deficiency or insulin resistance leading to many chronic complications. It is thus important to manage diabetes effectively in order to prevent and or delay these complications. Melatonin is produced by the pineal gland and regulates the wake-sleep circadian rhythm. Existing evidence suggests that melatonin may be effective in the management of DM. However, the evidence on the mechanism of the beneficial effect melatonin as a treatment for DM is limited. In this study, we investigated the effect of melatonin treatment on blood glucose, insulin (INS), AKT and superoxide dismutase (SOD) gene levels in diabetic rats. Non-diabetic and diabetic rats were treated orally for 4 weeks with either 25 mg or 50 mg/kg body weight of melatonin. At the end of the study, pancreatic and liver tissues morphology, glucose homeostasis, serum insulin and SOD levels, hepatic gene and protein expression of SOD as protecting antioxidant enzyme and AKT as central element involved in PI3K/AKT insulin signaling pathway were estimated. Melatonin treated diabetic rats showed reduced hyperglycemia, and increased serum insulin and SOD levels. In addition, melatonin induced an increased gene and protein expression of SOD and AKT. In conclusion, melatonin may play a role in treating diabetic rats via stimulation of insulin secretion, insulin signaling and reduction in oxidative stress.http://www.sciencedirect.com/science/article/pii/S240584402404670XDiabetes mellitusMelatoninInsulin signalingSOD and PI3K/AKT |
| spellingShingle | Mohamed Lotfy Aalaa Khattab Mohammed Shata Ahmad Alhasbani Abdallah Khalaf Saeed Alsaeedi Mahdi Thaker Hazza Said Harun Tumi Hassan Alzahmi Omar Alblooshi Mohamad Hamdan Amjad Hussein Biduth Kundu Ernest A. Adeghate Melatonin increases AKT and SOD gene and protein expressions in diabetic rats Heliyon Diabetes mellitus Melatonin Insulin signaling SOD and PI3K/AKT |
| title | Melatonin increases AKT and SOD gene and protein expressions in diabetic rats |
| title_full | Melatonin increases AKT and SOD gene and protein expressions in diabetic rats |
| title_fullStr | Melatonin increases AKT and SOD gene and protein expressions in diabetic rats |
| title_full_unstemmed | Melatonin increases AKT and SOD gene and protein expressions in diabetic rats |
| title_short | Melatonin increases AKT and SOD gene and protein expressions in diabetic rats |
| title_sort | melatonin increases akt and sod gene and protein expressions in diabetic rats |
| topic | Diabetes mellitus Melatonin Insulin signaling SOD and PI3K/AKT |
| url | http://www.sciencedirect.com/science/article/pii/S240584402404670X |
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